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Cytotoxicity of a novel nano-silver particle endodontic irrigant.

Chan EL, Zhang C, Cheung GS - Clin Cosmet Investig Dent (2015)

Bottom Line: Twelve culture wells (6-well plate) were divided into three groups (n=4).Toxicity of the test and control group on both mouse fibroblasts (P>0.05) and hPDLSCs (P=1.00) was not statistically different.Our results showed that the nano-silver irrigant was non-cytotoxic to both NIH 3T3 and hPDLSCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Health, Government of Hong Kong SAR, Hong Kong, Special Administrative Region, People's Republic of China.

ABSTRACT

Purpose: The aim of this study was to evaluate the cytotoxic effect of a novel nano-silver particle (25.2±6.5 nm) endodontic irrigant (0.2 mM) and compare it with 3% sodium hypochlorite.

Materials and methods: Two cell types, mouse fibroblast National Institutes of Health 3T3 (NIH 3T3) and primary human periodontal ligament stem cell (hPDLSCs) were used in a test for the effect of direct and indirect (by separating the agent and cell with a layer of agar) exposure to the two solutions. In the direct exposure experiment, ten groups of cell cultures were exposed to one dilution (3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6 or 1:7) of a nano-silver irrigant for 48 hours; the concentration-response function was estimated by determining the number of viable cells in each group by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The 50% lethal dose of the testing irrigant for NIH3T3 and hPDLSCs were estimated. In the second part of the experiment, a modified agar overlaying technique was applied. Twelve culture wells (6-well plate) were divided into three groups (n=4). The cell lysis zone (cytotoxic range) created by the stock nano-silver solution, 3% sodium hypochlorite, and an isotonic phosphate buffering saline (control) was measured by two double blinded observers (Kappa score =100%). The cytotoxic score of specific irrigant was derived by modified Sjögren's method.

Results: The 50% lethal doses of the testing nano silver irrigant for NIH 3T3 and hPDLSCs after 48 hours of direct exposure were 0.58 and 0.608 dilution of stock solution, respectively. The cytotoxic scores of nano-silver irrigant and control (phosphate buffered saline) on NIH 3T3 were 0.25 (95% confidence interval [CI] =0 to 1.04) and 0 (95% CI =0 to 0); and on hPDLSCs were 0.13 (95% CI =0 to 0.52) and 0.25 (95% CI =0 to 1.04), respectively. Toxicity of the test and control group on both mouse fibroblasts (P>0.05) and hPDLSCs (P=1.00) was not statistically different.

Conclusion: Our results showed that the nano-silver irrigant was non-cytotoxic to both NIH 3T3 and hPDLSCs.

No MeSH data available.


Related in: MedlinePlus

Survival curves (generated by the regression model) of hPDLSCs and Mouse fibroblast NIH3T3 (directly cultured for 48 hours with different dilutions of nano-silver).Abbreviations: hPDLSCs, human periodontal ligament stem cells; PDL, periodontal ligament.
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f8-ccide-7-065: Survival curves (generated by the regression model) of hPDLSCs and Mouse fibroblast NIH3T3 (directly cultured for 48 hours with different dilutions of nano-silver).Abbreviations: hPDLSCs, human periodontal ligament stem cells; PDL, periodontal ligament.

Mentions: Pearson correlation coefficient (r) of data set of both cell types was calculated as 0.884, P<0.001 (Figure 7), revealing a linear co-relation of dose-response relationship between them in direct contact mode. The dose-response relationship of both cell types appeared to be highly correlated (Figure 8).


Cytotoxicity of a novel nano-silver particle endodontic irrigant.

Chan EL, Zhang C, Cheung GS - Clin Cosmet Investig Dent (2015)

Survival curves (generated by the regression model) of hPDLSCs and Mouse fibroblast NIH3T3 (directly cultured for 48 hours with different dilutions of nano-silver).Abbreviations: hPDLSCs, human periodontal ligament stem cells; PDL, periodontal ligament.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507457&req=5

f8-ccide-7-065: Survival curves (generated by the regression model) of hPDLSCs and Mouse fibroblast NIH3T3 (directly cultured for 48 hours with different dilutions of nano-silver).Abbreviations: hPDLSCs, human periodontal ligament stem cells; PDL, periodontal ligament.
Mentions: Pearson correlation coefficient (r) of data set of both cell types was calculated as 0.884, P<0.001 (Figure 7), revealing a linear co-relation of dose-response relationship between them in direct contact mode. The dose-response relationship of both cell types appeared to be highly correlated (Figure 8).

Bottom Line: Twelve culture wells (6-well plate) were divided into three groups (n=4).Toxicity of the test and control group on both mouse fibroblasts (P>0.05) and hPDLSCs (P=1.00) was not statistically different.Our results showed that the nano-silver irrigant was non-cytotoxic to both NIH 3T3 and hPDLSCs.

View Article: PubMed Central - PubMed

Affiliation: Department of Health, Government of Hong Kong SAR, Hong Kong, Special Administrative Region, People's Republic of China.

ABSTRACT

Purpose: The aim of this study was to evaluate the cytotoxic effect of a novel nano-silver particle (25.2±6.5 nm) endodontic irrigant (0.2 mM) and compare it with 3% sodium hypochlorite.

Materials and methods: Two cell types, mouse fibroblast National Institutes of Health 3T3 (NIH 3T3) and primary human periodontal ligament stem cell (hPDLSCs) were used in a test for the effect of direct and indirect (by separating the agent and cell with a layer of agar) exposure to the two solutions. In the direct exposure experiment, ten groups of cell cultures were exposed to one dilution (3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6 or 1:7) of a nano-silver irrigant for 48 hours; the concentration-response function was estimated by determining the number of viable cells in each group by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The 50% lethal dose of the testing irrigant for NIH3T3 and hPDLSCs were estimated. In the second part of the experiment, a modified agar overlaying technique was applied. Twelve culture wells (6-well plate) were divided into three groups (n=4). The cell lysis zone (cytotoxic range) created by the stock nano-silver solution, 3% sodium hypochlorite, and an isotonic phosphate buffering saline (control) was measured by two double blinded observers (Kappa score =100%). The cytotoxic score of specific irrigant was derived by modified Sjögren's method.

Results: The 50% lethal doses of the testing nano silver irrigant for NIH 3T3 and hPDLSCs after 48 hours of direct exposure were 0.58 and 0.608 dilution of stock solution, respectively. The cytotoxic scores of nano-silver irrigant and control (phosphate buffered saline) on NIH 3T3 were 0.25 (95% confidence interval [CI] =0 to 1.04) and 0 (95% CI =0 to 0); and on hPDLSCs were 0.13 (95% CI =0 to 0.52) and 0.25 (95% CI =0 to 1.04), respectively. Toxicity of the test and control group on both mouse fibroblasts (P>0.05) and hPDLSCs (P=1.00) was not statistically different.

Conclusion: Our results showed that the nano-silver irrigant was non-cytotoxic to both NIH 3T3 and hPDLSCs.

No MeSH data available.


Related in: MedlinePlus