Limits...
Effect of deworming on Th2 immune response during HIV-helminths co-infection.

Mulu A, Anagaw B, Gelaw A, Ota F, Kassu A, Yifru S - J Transl Med (2015)

Bottom Line: Helminths infections have been suggested to worsen the outcome of HIV infection by polarizing the immune response towards Th2.This difference was also markedly observed between symptomatic HIV infected patients after with and without helminths infection (1,690 [IQR 1,116-2,491] versus 1,252 [703-2,251] IU/ml; P = 0.047).Thus, deworming should be integrated with ART program in helminths endemic areas of tropical countries.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia. andargachewmulu@yahoo.com.

ABSTRACT

Background: Helminths infections have been suggested to worsen the outcome of HIV infection by polarizing the immune response towards Th2. The purpose of this study is to determine the activity of Th2 immune response by measuring total serum IgE level during symptomatic and asymptomatic HIV infection with and without helminths co-infection and to define the role of deworming and/or ART on kinetics of serum IgE.

Methods: This prospective comparative study was conducted among symptomatic HIV-1 infected adults, treatment naïve asymptomatic HIV positive individuals and HIV negative apparently healthy controls with and without helminths co-infection. Detection and quantification of helminths and determination of serum IgE level, CD4(+), and CD8(+) T cell count were done at baseline and 12 weeks after ART and/or deworming.

Results: HIV patients co-infected with helminths showed a high level of serum IgE compared to HIV patients without helminths co-infection (1,688 [IQR 721-2,473] versus 1,221 [IQR 618-2,289] IU/ml; P = 0.022). This difference was also markedly observed between symptomatic HIV infected patients after with and without helminths infection (1,690 [IQR 1,116-2,491] versus 1,252 [703-2,251] IU/ml; P = 0.047). A significant decline in serum IgE level was observed 12 weeks after deworming and ART of symptomatic HIV infected patients with (1,487 versus 992, P = 0.002) and without (1,233 versus 976 IU/ml, P = 0.093) helminths co-infection. However, there was no significant decrease in serum IgE level among asymptomatic HIV infected individuals (1,183 versus 1,097 IU/ml, P = 0.13) and apparently health controls (666 IU/ml versus 571, P = 0.09) without helminths co-infection 12 weeks after deworming.

Conclusions: The significant decline of serum IgE level 12 weeks after deworming of both symptomatic and asymptomatic patients indicate a tendency to down-regulate the Th2 immune response and is additional supportive evidence that deworming positively impacts HIV/AIDS diseases progression. Thus, deworming should be integrated with ART program in helminths endemic areas of tropical countries.

No MeSH data available.


Related in: MedlinePlus

Comparison of changes in median total serum IgE at baseline (before treatment) and at 12 weeks after successful deworming in symptomatic, asymptomatic and health controls. Horizontal lines are medians and interquartile ranges (IQRs) (25 and 75 centile) and the black dot represents outlier values. Keys: Similar to Figure 2
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4506626&req=5

Fig3: Comparison of changes in median total serum IgE at baseline (before treatment) and at 12 weeks after successful deworming in symptomatic, asymptomatic and health controls. Horizontal lines are medians and interquartile ranges (IQRs) (25 and 75 centile) and the black dot represents outlier values. Keys: Similar to Figure 2

Mentions: As indicated in Figure 3, a significant decline in serum IgE level was observed 12 weeks after deworming and ART of symptomatic HIV infected patients with (1,487 versus 992, P = 0.002) and without (1,233 versus 976 IU/ml, P = 0.093) helminths co-infection. Similarly, serum IgE level was significantly reduced among asymptomatic HIV infected individuals (1,261 versus 996 IU/ml, P = 0.0001) and apparently health controls (993 versus 646 IU/ml, P = 0.0002) with helminths coinfection 12 weeks after deworming. However, there was no significant decrease in serum IgE level among asymptomatic HIV infected individuals (1,183 versus 1,097 IU/ml, P = 0.13) and apparently health controls (666 IU/ml versus 571, P = 0.09) without helminths co-infection 12 weeks after deworming. After controlling ART status and helminths coinfection a significant reduction in serum IgE level was observed in all groups (data not shown).Figure 3


Effect of deworming on Th2 immune response during HIV-helminths co-infection.

Mulu A, Anagaw B, Gelaw A, Ota F, Kassu A, Yifru S - J Transl Med (2015)

Comparison of changes in median total serum IgE at baseline (before treatment) and at 12 weeks after successful deworming in symptomatic, asymptomatic and health controls. Horizontal lines are medians and interquartile ranges (IQRs) (25 and 75 centile) and the black dot represents outlier values. Keys: Similar to Figure 2
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4506626&req=5

Fig3: Comparison of changes in median total serum IgE at baseline (before treatment) and at 12 weeks after successful deworming in symptomatic, asymptomatic and health controls. Horizontal lines are medians and interquartile ranges (IQRs) (25 and 75 centile) and the black dot represents outlier values. Keys: Similar to Figure 2
Mentions: As indicated in Figure 3, a significant decline in serum IgE level was observed 12 weeks after deworming and ART of symptomatic HIV infected patients with (1,487 versus 992, P = 0.002) and without (1,233 versus 976 IU/ml, P = 0.093) helminths co-infection. Similarly, serum IgE level was significantly reduced among asymptomatic HIV infected individuals (1,261 versus 996 IU/ml, P = 0.0001) and apparently health controls (993 versus 646 IU/ml, P = 0.0002) with helminths coinfection 12 weeks after deworming. However, there was no significant decrease in serum IgE level among asymptomatic HIV infected individuals (1,183 versus 1,097 IU/ml, P = 0.13) and apparently health controls (666 IU/ml versus 571, P = 0.09) without helminths co-infection 12 weeks after deworming. After controlling ART status and helminths coinfection a significant reduction in serum IgE level was observed in all groups (data not shown).Figure 3

Bottom Line: Helminths infections have been suggested to worsen the outcome of HIV infection by polarizing the immune response towards Th2.This difference was also markedly observed between symptomatic HIV infected patients after with and without helminths infection (1,690 [IQR 1,116-2,491] versus 1,252 [703-2,251] IU/ml; P = 0.047).Thus, deworming should be integrated with ART program in helminths endemic areas of tropical countries.

View Article: PubMed Central - PubMed

Affiliation: Department of Microbiology, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia. andargachewmulu@yahoo.com.

ABSTRACT

Background: Helminths infections have been suggested to worsen the outcome of HIV infection by polarizing the immune response towards Th2. The purpose of this study is to determine the activity of Th2 immune response by measuring total serum IgE level during symptomatic and asymptomatic HIV infection with and without helminths co-infection and to define the role of deworming and/or ART on kinetics of serum IgE.

Methods: This prospective comparative study was conducted among symptomatic HIV-1 infected adults, treatment naïve asymptomatic HIV positive individuals and HIV negative apparently healthy controls with and without helminths co-infection. Detection and quantification of helminths and determination of serum IgE level, CD4(+), and CD8(+) T cell count were done at baseline and 12 weeks after ART and/or deworming.

Results: HIV patients co-infected with helminths showed a high level of serum IgE compared to HIV patients without helminths co-infection (1,688 [IQR 721-2,473] versus 1,221 [IQR 618-2,289] IU/ml; P = 0.022). This difference was also markedly observed between symptomatic HIV infected patients after with and without helminths infection (1,690 [IQR 1,116-2,491] versus 1,252 [703-2,251] IU/ml; P = 0.047). A significant decline in serum IgE level was observed 12 weeks after deworming and ART of symptomatic HIV infected patients with (1,487 versus 992, P = 0.002) and without (1,233 versus 976 IU/ml, P = 0.093) helminths co-infection. However, there was no significant decrease in serum IgE level among asymptomatic HIV infected individuals (1,183 versus 1,097 IU/ml, P = 0.13) and apparently health controls (666 IU/ml versus 571, P = 0.09) without helminths co-infection 12 weeks after deworming.

Conclusions: The significant decline of serum IgE level 12 weeks after deworming of both symptomatic and asymptomatic patients indicate a tendency to down-regulate the Th2 immune response and is additional supportive evidence that deworming positively impacts HIV/AIDS diseases progression. Thus, deworming should be integrated with ART program in helminths endemic areas of tropical countries.

No MeSH data available.


Related in: MedlinePlus