Cytokinetic Failure-induced Tetraploidy Develops into Aneuploidy, Triggering Skin Aging in Phosphovimentin-deficient Mice.
Bottom Line: Early into wound healing, subcutaneous fibroblasts failed to undergo cytokinesis, resulting in binucleate tetraploidy.Thereafter, senescence-related markers were significantly elevated in VIM(SA/SA) mice.Because our tetraploidy-prone mouse model also exhibited subcutaneous fat loss at the age of 14 months, another premature aging phenotype, our data suggest that following cytokinetic failure, a subset of tetraploid cells enters a new cell cycle and develops into aneuploid cells in vivo, which promote premature aging.
Affiliation: From the Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya 464-8681.Show MeSH
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Mentions: Antibody signals in a digital image of Figs. 5C and 6B were calculated as described previously (39). All data were shown as mean ± S.E. All p values (*, p < 0.05; **, p < 0.01; ***, p < 0.001) were determined by two-tailed Student's t test (Graph Pad software), compared with wild-type (VIMWT/WT) mice.
Affiliation: From the Division of Biochemistry, Aichi Cancer Center Research Institute, Nagoya 464-8681.