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Effects of Pueraria lobata Root Ethanol Extract on Adipogenesis and Lipogenesis During 3T3-L1 Differentiation into Adipocytes.

Lee CM, Yoon MS, Kim YC - Toxicol Res (2015)

Bottom Line: In contrast, the lipid content of PLREE-treated cells was significantly reduced by 7.8% (p < 0.05), 35.6% (p < 0.001), and 42.2% (p < 0.001) following treatment with 100, 250, and 500 μg/mL PLREE, respectively, as compared to differentiated control cells.PLREE upregulated adipose triglyceride lipase mRNA and protein expression, and hormone-sensitive lipase (HSL) protein expression, but did not affect HSL mRNA expression.In conclusion, we found that PLREE enhanced adipogenesis, but reduced lipogenesis, resulting in decreased lipid accumulation in 3T3-L1 cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Beauty Cooperation, Keimyung College University, Daegu, Korea.

ABSTRACT
We evaluated the inhibitory effect of Pueraria lobata root ethanol extract (PLREE) on lipid accumulation during 3T3-L1 differentiation to adipocytes by measuring the intracellular expression of adipogenic, lipogenic, and lipolytic markers and lipid accumulation. The total polyphenol and flavonoid content of PLREE were 47 and 29 mg/g, respectively. The electron donating capacity of PLREE at 1,000 μg/mL was 48.8%. Treatment of 3T3-L1 preadipocytes with 100, 250, or 500 μg/mL PLREE for 8 days dose-dependently promoted the differentiation of 3T3-L1 cells. In contrast, the lipid content of PLREE-treated cells was significantly reduced by 7.8% (p < 0.05), 35.6% (p < 0.001), and 42.2% (p < 0.001) following treatment with 100, 250, and 500 μg/mL PLREE, respectively, as compared to differentiated control cells. PLREE upregulated peroxisome proliferator-activated receptor γ mRNA and protein, and sterol regulator element-binding protein-1c mRNA levels, but did not affect CCAAT/enhancer binding-protein β and α mRNA levels. PLREE also downregulated acetyl-CoA carboxylase mRNA and protein, fatty acid synthase (FAS) protein, and leptin mRNA levels, but did not affect FAS mRNA expression. PLREE upregulated adipose triglyceride lipase mRNA and protein expression, and hormone-sensitive lipase (HSL) protein expression, but did not affect HSL mRNA expression. In conclusion, we found that PLREE enhanced adipogenesis, but reduced lipogenesis, resulting in decreased lipid accumulation in 3T3-L1 cells.

No MeSH data available.


Effect of PLREE on lipid accumulation during the differentiation of 3T3-L1 preadipocytes into adipocytes. 3T3-L1 preadipocytes were treated with PLREE at 100 μg/mL (C), 250 μg/mL (D), and 500 μg/mL (E) for 8 days, and microscopic images of Oil red O-stained 3T3-L1 cells (day 8) were compared to undifferentiated normal cells (A) and differentiated control cells (B). 200× magnification.
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Figure 006: Effect of PLREE on lipid accumulation during the differentiation of 3T3-L1 preadipocytes into adipocytes. 3T3-L1 preadipocytes were treated with PLREE at 100 μg/mL (C), 250 μg/mL (D), and 500 μg/mL (E) for 8 days, and microscopic images of Oil red O-stained 3T3-L1 cells (day 8) were compared to undifferentiated normal cells (A) and differentiated control cells (B). 200× magnification.

Mentions: On day 8, the degree of lipid accumulation was assessed by Oil red O staining and imaging (Fig. 6). Undifferentiated cells displayed little lipid accumulation, whereas differentiated control cells were observed with many lipid droplets. However, PLREE-treatment dose-dependently reduced lipid droplets, as compared to differentiated control cells. Quantitative analysis revealed that the lipid content of PLREE-treated cells was significantly and dosedependently reduced at 100, 250, and 500 μg/mL by 7.8% (p < 0.05), 35.6% (p < 0.001), and 42.2% (p < 0.001), respectively (Fig. 7).


Effects of Pueraria lobata Root Ethanol Extract on Adipogenesis and Lipogenesis During 3T3-L1 Differentiation into Adipocytes.

Lee CM, Yoon MS, Kim YC - Toxicol Res (2015)

Effect of PLREE on lipid accumulation during the differentiation of 3T3-L1 preadipocytes into adipocytes. 3T3-L1 preadipocytes were treated with PLREE at 100 μg/mL (C), 250 μg/mL (D), and 500 μg/mL (E) for 8 days, and microscopic images of Oil red O-stained 3T3-L1 cells (day 8) were compared to undifferentiated normal cells (A) and differentiated control cells (B). 200× magnification.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4505350&req=5

Figure 006: Effect of PLREE on lipid accumulation during the differentiation of 3T3-L1 preadipocytes into adipocytes. 3T3-L1 preadipocytes were treated with PLREE at 100 μg/mL (C), 250 μg/mL (D), and 500 μg/mL (E) for 8 days, and microscopic images of Oil red O-stained 3T3-L1 cells (day 8) were compared to undifferentiated normal cells (A) and differentiated control cells (B). 200× magnification.
Mentions: On day 8, the degree of lipid accumulation was assessed by Oil red O staining and imaging (Fig. 6). Undifferentiated cells displayed little lipid accumulation, whereas differentiated control cells were observed with many lipid droplets. However, PLREE-treatment dose-dependently reduced lipid droplets, as compared to differentiated control cells. Quantitative analysis revealed that the lipid content of PLREE-treated cells was significantly and dosedependently reduced at 100, 250, and 500 μg/mL by 7.8% (p < 0.05), 35.6% (p < 0.001), and 42.2% (p < 0.001), respectively (Fig. 7).

Bottom Line: In contrast, the lipid content of PLREE-treated cells was significantly reduced by 7.8% (p < 0.05), 35.6% (p < 0.001), and 42.2% (p < 0.001) following treatment with 100, 250, and 500 μg/mL PLREE, respectively, as compared to differentiated control cells.PLREE upregulated adipose triglyceride lipase mRNA and protein expression, and hormone-sensitive lipase (HSL) protein expression, but did not affect HSL mRNA expression.In conclusion, we found that PLREE enhanced adipogenesis, but reduced lipogenesis, resulting in decreased lipid accumulation in 3T3-L1 cells.

View Article: PubMed Central - PubMed

Affiliation: Division of Beauty Cooperation, Keimyung College University, Daegu, Korea.

ABSTRACT
We evaluated the inhibitory effect of Pueraria lobata root ethanol extract (PLREE) on lipid accumulation during 3T3-L1 differentiation to adipocytes by measuring the intracellular expression of adipogenic, lipogenic, and lipolytic markers and lipid accumulation. The total polyphenol and flavonoid content of PLREE were 47 and 29 mg/g, respectively. The electron donating capacity of PLREE at 1,000 μg/mL was 48.8%. Treatment of 3T3-L1 preadipocytes with 100, 250, or 500 μg/mL PLREE for 8 days dose-dependently promoted the differentiation of 3T3-L1 cells. In contrast, the lipid content of PLREE-treated cells was significantly reduced by 7.8% (p < 0.05), 35.6% (p < 0.001), and 42.2% (p < 0.001) following treatment with 100, 250, and 500 μg/mL PLREE, respectively, as compared to differentiated control cells. PLREE upregulated peroxisome proliferator-activated receptor γ mRNA and protein, and sterol regulator element-binding protein-1c mRNA levels, but did not affect CCAAT/enhancer binding-protein β and α mRNA levels. PLREE also downregulated acetyl-CoA carboxylase mRNA and protein, fatty acid synthase (FAS) protein, and leptin mRNA levels, but did not affect FAS mRNA expression. PLREE upregulated adipose triglyceride lipase mRNA and protein expression, and hormone-sensitive lipase (HSL) protein expression, but did not affect HSL mRNA expression. In conclusion, we found that PLREE enhanced adipogenesis, but reduced lipogenesis, resulting in decreased lipid accumulation in 3T3-L1 cells.

No MeSH data available.