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Anti-aging Effect and Gene Expression Profiling of Aged Rats Treated with G. bimaculatus Extract.

Ahn MY, Hwang JS, Yun EY, Kim MJ, Park KK - Toxicol Res (2015)

Bottom Line: Extract from Gryllus bimaculatus crickets inhibits oxidation at the DNA level, with reduced production of 8-hydroxy-2'-deoxyguanosine (8-OHdG).The extract produced a meaningful anti-edema effect, evident by the inhibition of creatinine phosphokinase activity.Data suggest that G. bimaculatus extract may have value in lessening the effects of aging, resulting in a differential gene expression pattern indicative of a marked stress response and lower expression of metabolic and biosynthetic genes.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju-Gun, Korea.

ABSTRACT
Extract from Gryllus bimaculatus crickets inhibits oxidation at the DNA level, with reduced production of 8-hydroxy-2'-deoxyguanosine (8-OHdG). Microarray analyses were performed with a rat 28K cDNA clone set array to identify the gene expression profiles of aged (10 months old) Wistar Kyoto rats treated for one month with 100 mg/kg G. bimaculatus ethanol extract to assess the effects. The extract produced a meaningful anti-edema effect, evident by the inhibition of creatinine phosphokinase activity. The weights of abdominal and ovarian adipose tissues were reduced and the proportion of unsaturated fatty acids in adipose tissues was increased in an extract dose-dependent manner. Compared with untreated control rats, rats treated with the extract displayed the upregulation of 1053 genes including Fas (tumor necrosis factor receptor superfamily, member 6), Amigo3 (adhesion molecule with an immunoglobulin-like domain), Reticulon 4, 3-hydroxy-3-methylglutaryl-coenzyme (Hmgcr; a reductase), related anti-fatigue (enzyme metabolism), and Rtn antioxidant, and the downregulation of 73 genes including Ugt2b (UDP glycosyltransferase 2 family), Early growth response 1, and Glycoprotein m6a. Data suggest that G. bimaculatus extract may have value in lessening the effects of aging, resulting in a differential gene expression pattern indicative of a marked stress response and lower expression of metabolic and biosynthetic genes.

No MeSH data available.


Related in: MedlinePlus

Effect of Gb extract on body weight and systolic blood pressure in 10-month old WKY rats treated orally with Gb ethanol extract for 1 month
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Figure 001: Effect of Gb extract on body weight and systolic blood pressure in 10-month old WKY rats treated orally with Gb ethanol extract for 1 month

Mentions: Body weight, blood pressure, and heart rate changes. There were no toxicologically significant differences in the mean body weight between the treatment groups (Fig. 1). During the 1-month administration period, the body weights of the male WKY rats in the treatment and control groups were comparable. The mean weekly body weight versus time data are presented in Fig. 1. No statistically significant differences in blood pressure (systolic blood pressure and heart rate) were observed between the 100 mg/kg G. bimaculatus extract-treated group and the control group.


Anti-aging Effect and Gene Expression Profiling of Aged Rats Treated with G. bimaculatus Extract.

Ahn MY, Hwang JS, Yun EY, Kim MJ, Park KK - Toxicol Res (2015)

Effect of Gb extract on body weight and systolic blood pressure in 10-month old WKY rats treated orally with Gb ethanol extract for 1 month
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4505348&req=5

Figure 001: Effect of Gb extract on body weight and systolic blood pressure in 10-month old WKY rats treated orally with Gb ethanol extract for 1 month
Mentions: Body weight, blood pressure, and heart rate changes. There were no toxicologically significant differences in the mean body weight between the treatment groups (Fig. 1). During the 1-month administration period, the body weights of the male WKY rats in the treatment and control groups were comparable. The mean weekly body weight versus time data are presented in Fig. 1. No statistically significant differences in blood pressure (systolic blood pressure and heart rate) were observed between the 100 mg/kg G. bimaculatus extract-treated group and the control group.

Bottom Line: Extract from Gryllus bimaculatus crickets inhibits oxidation at the DNA level, with reduced production of 8-hydroxy-2'-deoxyguanosine (8-OHdG).The extract produced a meaningful anti-edema effect, evident by the inhibition of creatinine phosphokinase activity.Data suggest that G. bimaculatus extract may have value in lessening the effects of aging, resulting in a differential gene expression pattern indicative of a marked stress response and lower expression of metabolic and biosynthetic genes.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural Biology, National Academy of Agricultural Science, RDA, Wanju-Gun, Korea.

ABSTRACT
Extract from Gryllus bimaculatus crickets inhibits oxidation at the DNA level, with reduced production of 8-hydroxy-2'-deoxyguanosine (8-OHdG). Microarray analyses were performed with a rat 28K cDNA clone set array to identify the gene expression profiles of aged (10 months old) Wistar Kyoto rats treated for one month with 100 mg/kg G. bimaculatus ethanol extract to assess the effects. The extract produced a meaningful anti-edema effect, evident by the inhibition of creatinine phosphokinase activity. The weights of abdominal and ovarian adipose tissues were reduced and the proportion of unsaturated fatty acids in adipose tissues was increased in an extract dose-dependent manner. Compared with untreated control rats, rats treated with the extract displayed the upregulation of 1053 genes including Fas (tumor necrosis factor receptor superfamily, member 6), Amigo3 (adhesion molecule with an immunoglobulin-like domain), Reticulon 4, 3-hydroxy-3-methylglutaryl-coenzyme (Hmgcr; a reductase), related anti-fatigue (enzyme metabolism), and Rtn antioxidant, and the downregulation of 73 genes including Ugt2b (UDP glycosyltransferase 2 family), Early growth response 1, and Glycoprotein m6a. Data suggest that G. bimaculatus extract may have value in lessening the effects of aging, resulting in a differential gene expression pattern indicative of a marked stress response and lower expression of metabolic and biosynthetic genes.

No MeSH data available.


Related in: MedlinePlus