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Cornus officinalis Methanol Extract Upregulates Melanogenesis in Melan-a Cells.

An YA, Hwang JY, Lee JS, Kim YC - Toxicol Res (2015)

Bottom Line: Treatment with 12.5 μg/mL of COME significantly increased melanin content by 36.1% (p < 0.001) to a level even higher than that (31.6%) of 3-isobutyl-1-methyl-xanthine, a well-known pigmentation agent.In addition, COME upregulated the expression of tyrosinase-related proteins 1 and 2 and microphthalmia-associated transcription factor-M messenger RNA expression.These results imply that COME may be appropriate for development as a natural product to treat hair graying.

View Article: PubMed Central - PubMed

Affiliation: Major in Public Health, Faculty of Food & Health Sciences, Keimyung University, Daegu, Korea.

ABSTRACT
Cornus officinalis is widely distributed in Korea, and its fruit has been used to make as herbal drug for traditional medicine in Korea, Japan, and China because of its tonic, analgesic, and diuretic properties. However, the effects of C. officinalis methanol extract (COME) on melanogenesis remain poorly understood. We evaluated the melanogenic capability of COME in melan-a cells, which are immortalized mouse melanocytes. COME increased melanin synthesis in a dose-dependent manner. Treatment with 12.5 μg/mL of COME significantly increased melanin content by 36.1% (p < 0.001) to a level even higher than that (31.6%) of 3-isobutyl-1-methyl-xanthine, a well-known pigmentation agent. COME also upregulated tyrosinase activity and its messenger RNA and protein expression. In addition, COME upregulated the expression of tyrosinase-related proteins 1 and 2 and microphthalmia-associated transcription factor-M messenger RNA expression. These results imply that COME may be appropriate for development as a natural product to treat hair graying.

No MeSH data available.


Related in: MedlinePlus

Cell viability of melan-a cells treated with 3-isobutyl-1-methylxanthine (IBMX) and Cornus officinalis methanol extract (COME). Values are means ± SD of three measurements.
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Figure 003: Cell viability of melan-a cells treated with 3-isobutyl-1-methylxanthine (IBMX) and Cornus officinalis methanol extract (COME). Values are means ± SD of three measurements.

Mentions: Cytotoxicity of COME in melan-a cells. Greater than 80% cell viability was observed at COME concentrations between 3.125 and 12.5 μg/mL, but cell viability was reduced to 75.5% at 25 μg/mL (Fig. 3). Therefore, the maximum permissible level for COME application to melan-a cells was 12.5 μg/mL. The maximum permissible level for IBMX application was also 12.5 μg/mL.


Cornus officinalis Methanol Extract Upregulates Melanogenesis in Melan-a Cells.

An YA, Hwang JY, Lee JS, Kim YC - Toxicol Res (2015)

Cell viability of melan-a cells treated with 3-isobutyl-1-methylxanthine (IBMX) and Cornus officinalis methanol extract (COME). Values are means ± SD of three measurements.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4505347&req=5

Figure 003: Cell viability of melan-a cells treated with 3-isobutyl-1-methylxanthine (IBMX) and Cornus officinalis methanol extract (COME). Values are means ± SD of three measurements.
Mentions: Cytotoxicity of COME in melan-a cells. Greater than 80% cell viability was observed at COME concentrations between 3.125 and 12.5 μg/mL, but cell viability was reduced to 75.5% at 25 μg/mL (Fig. 3). Therefore, the maximum permissible level for COME application to melan-a cells was 12.5 μg/mL. The maximum permissible level for IBMX application was also 12.5 μg/mL.

Bottom Line: Treatment with 12.5 μg/mL of COME significantly increased melanin content by 36.1% (p < 0.001) to a level even higher than that (31.6%) of 3-isobutyl-1-methyl-xanthine, a well-known pigmentation agent.In addition, COME upregulated the expression of tyrosinase-related proteins 1 and 2 and microphthalmia-associated transcription factor-M messenger RNA expression.These results imply that COME may be appropriate for development as a natural product to treat hair graying.

View Article: PubMed Central - PubMed

Affiliation: Major in Public Health, Faculty of Food & Health Sciences, Keimyung University, Daegu, Korea.

ABSTRACT
Cornus officinalis is widely distributed in Korea, and its fruit has been used to make as herbal drug for traditional medicine in Korea, Japan, and China because of its tonic, analgesic, and diuretic properties. However, the effects of C. officinalis methanol extract (COME) on melanogenesis remain poorly understood. We evaluated the melanogenic capability of COME in melan-a cells, which are immortalized mouse melanocytes. COME increased melanin synthesis in a dose-dependent manner. Treatment with 12.5 μg/mL of COME significantly increased melanin content by 36.1% (p < 0.001) to a level even higher than that (31.6%) of 3-isobutyl-1-methyl-xanthine, a well-known pigmentation agent. COME also upregulated tyrosinase activity and its messenger RNA and protein expression. In addition, COME upregulated the expression of tyrosinase-related proteins 1 and 2 and microphthalmia-associated transcription factor-M messenger RNA expression. These results imply that COME may be appropriate for development as a natural product to treat hair graying.

No MeSH data available.


Related in: MedlinePlus