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Retinopathy Induced by Zinc Oxide Nanoparticles in Rats Assessed by Micro-computed Tomography and Histopathology.

Kim YH, Kwak KA, Kim TS, Seok JH, Roh HS, Lee JK, Jeong J, Meang EH, Hong JS, Lee YS, Kang JS - Toxicol Res (2015)

Bottom Line: Ocular samples were prepared from animals treated for 90 days (10 males and 10 females, respectively) and from recovery animals (5 males and 5 females, respectively) sacrificed at 14 days after final treatment and were compared to age-matched control animals.Neuronal nuclei expression was decreased in neurons of the ganglion cell layer of animals treated with ZnO NPs compared to the control group.Taken together, treatment with 20-nm, negatively-charged ZnO NPs increased retinopathy, associated with local distribution of them in ocular lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Laboratory Science, Namseoul University, Cheonan, Korea.

ABSTRACT
Nanotechnology has advanced at an extremely rapid pace over the past several years in numerous fields of research. However, the uptake of nanoparticles (NPs) into the body after administration through various routes may pose a risk to human health. In this study, we investigated the potential ocular toxicity of 20-nm, negatively- charged zinc oxide (ZnO) NPs in rats using micro-computed tomography (micro-CT) and histopathological assessment. Animals were divided into four groups as control group, ZnO NPs treatment group (500 mg/kg/day), control recovery group, and ZnO NPs treatment and recovery group. Ocular samples were prepared from animals treated for 90 days (10 males and 10 females, respectively) and from recovery animals (5 males and 5 females, respectively) sacrificed at 14 days after final treatment and were compared to age-matched control animals. Micro-CT analyses represented the deposition and distribution of foreign materials in the eyes of rats treated with ZnO NPs, whereas control animals showed no such findings. X-ray fluorescence spectrometry and energy dispersive spectrometry showed the intraocular foreign materials as zinc in treated rats, whereas control animals showed no zinc signal. Histopathological examination revealed the retinopathy in the eyes of rats treated with ZnO NPs. Neuronal nuclei expression was decreased in neurons of the ganglion cell layer of animals treated with ZnO NPs compared to the control group. Taken together, treatment with 20-nm, negatively-charged ZnO NPs increased retinopathy, associated with local distribution of them in ocular lesions.

No MeSH data available.


Related in: MedlinePlus

Analysis of eyeball on 3D image of micro-CT. (A) vehicle treated groups for 90 days; (B) ZnO NPs treated groups for 90 days; (C) vehicle treated groups for 90 days plus a 14-day recovery period; (D) ZnO NPs treated groups for 90 days plus a 14-day recovery period. Note observation of deposition and distribution of foreign materials (red arrow) in eye of rat treated with ZnO NPs.
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Figure 002: Analysis of eyeball on 3D image of micro-CT. (A) vehicle treated groups for 90 days; (B) ZnO NPs treated groups for 90 days; (C) vehicle treated groups for 90 days plus a 14-day recovery period; (D) ZnO NPs treated groups for 90 days plus a 14-day recovery period. Note observation of deposition and distribution of foreign materials (red arrow) in eye of rat treated with ZnO NPs.

Mentions: Using micro-CT 3D image analysis, the animals treated with vehicle (G1) for 90 days and 90 days plus 14-day recovery (G3) showed no foreign materials (Fig. 2A and 2C, respectively). Conversely, particles were deposited in the eye ball and muscle and surrounding tissues of animals treated with ZnO NPs (G2) for 90 days and 90 days plus 14-day recovery (G4) (Fig. 2B and 2D, respectively).


Retinopathy Induced by Zinc Oxide Nanoparticles in Rats Assessed by Micro-computed Tomography and Histopathology.

Kim YH, Kwak KA, Kim TS, Seok JH, Roh HS, Lee JK, Jeong J, Meang EH, Hong JS, Lee YS, Kang JS - Toxicol Res (2015)

Analysis of eyeball on 3D image of micro-CT. (A) vehicle treated groups for 90 days; (B) ZnO NPs treated groups for 90 days; (C) vehicle treated groups for 90 days plus a 14-day recovery period; (D) ZnO NPs treated groups for 90 days plus a 14-day recovery period. Note observation of deposition and distribution of foreign materials (red arrow) in eye of rat treated with ZnO NPs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4505346&req=5

Figure 002: Analysis of eyeball on 3D image of micro-CT. (A) vehicle treated groups for 90 days; (B) ZnO NPs treated groups for 90 days; (C) vehicle treated groups for 90 days plus a 14-day recovery period; (D) ZnO NPs treated groups for 90 days plus a 14-day recovery period. Note observation of deposition and distribution of foreign materials (red arrow) in eye of rat treated with ZnO NPs.
Mentions: Using micro-CT 3D image analysis, the animals treated with vehicle (G1) for 90 days and 90 days plus 14-day recovery (G3) showed no foreign materials (Fig. 2A and 2C, respectively). Conversely, particles were deposited in the eye ball and muscle and surrounding tissues of animals treated with ZnO NPs (G2) for 90 days and 90 days plus 14-day recovery (G4) (Fig. 2B and 2D, respectively).

Bottom Line: Ocular samples were prepared from animals treated for 90 days (10 males and 10 females, respectively) and from recovery animals (5 males and 5 females, respectively) sacrificed at 14 days after final treatment and were compared to age-matched control animals.Neuronal nuclei expression was decreased in neurons of the ganglion cell layer of animals treated with ZnO NPs compared to the control group.Taken together, treatment with 20-nm, negatively-charged ZnO NPs increased retinopathy, associated with local distribution of them in ocular lesions.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Laboratory Science, Namseoul University, Cheonan, Korea.

ABSTRACT
Nanotechnology has advanced at an extremely rapid pace over the past several years in numerous fields of research. However, the uptake of nanoparticles (NPs) into the body after administration through various routes may pose a risk to human health. In this study, we investigated the potential ocular toxicity of 20-nm, negatively- charged zinc oxide (ZnO) NPs in rats using micro-computed tomography (micro-CT) and histopathological assessment. Animals were divided into four groups as control group, ZnO NPs treatment group (500 mg/kg/day), control recovery group, and ZnO NPs treatment and recovery group. Ocular samples were prepared from animals treated for 90 days (10 males and 10 females, respectively) and from recovery animals (5 males and 5 females, respectively) sacrificed at 14 days after final treatment and were compared to age-matched control animals. Micro-CT analyses represented the deposition and distribution of foreign materials in the eyes of rats treated with ZnO NPs, whereas control animals showed no such findings. X-ray fluorescence spectrometry and energy dispersive spectrometry showed the intraocular foreign materials as zinc in treated rats, whereas control animals showed no zinc signal. Histopathological examination revealed the retinopathy in the eyes of rats treated with ZnO NPs. Neuronal nuclei expression was decreased in neurons of the ganglion cell layer of animals treated with ZnO NPs compared to the control group. Taken together, treatment with 20-nm, negatively-charged ZnO NPs increased retinopathy, associated with local distribution of them in ocular lesions.

No MeSH data available.


Related in: MedlinePlus