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First Report on Fusarium Wilt of Zucchini Caused by Fusarium oxysporum, in Korea.

Choi IY, Kim JH, Lee WH, Park JH, Shin HD - Mycobiology (2015)

Bottom Line: Infected plants eventually died during growth.Pathogenicity of a representative isolate was demonstrated via artificial inoculation, and it satisfied Koch's postulates.To our knowledge, this is the first report of F. oxysporum causing wilt of zucchini in Korea.

View Article: PubMed Central - PubMed

Affiliation: Jeollabuk-do Agricultural Research and Extension Services, Iksan 570-704, Korea.

ABSTRACT
Fusarium wilt of zucchini in Jeonju, Korea, was first noticed in May 2013. Symptoms included wilting of the foliage, drying and withering of older leaves, and stunting of plants. Infected plants eventually died during growth. Based on morphological characteristics and phylogenetic analyses of the molecular markers (internal transcribed spacer rDNA and translation elongation factor 1α), the fungus was identified as Fusarium oxysporum. Pathogenicity of a representative isolate was demonstrated via artificial inoculation, and it satisfied Koch's postulates. To our knowledge, this is the first report of F. oxysporum causing wilt of zucchini in Korea.

No MeSH data available.


Phylogenetic analysis by the neighbor-joining method comparing the sequence of the internal transcribed spacer ribosomal DNA (rDNA) region from Fusarium oxysporum with that of other Fusarium spp. obtained from GenBank. The numbers above the branches represent the bootstrap value. The fungal strains identified in this study are shown in boldface.
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Figure 2: Phylogenetic analysis by the neighbor-joining method comparing the sequence of the internal transcribed spacer ribosomal DNA (rDNA) region from Fusarium oxysporum with that of other Fusarium spp. obtained from GenBank. The numbers above the branches represent the bootstrap value. The fungal strains identified in this study are shown in boldface.

Mentions: The ITS and TEF sequences from a representative isolate were deposited in GenBank with the accession numbers KF372590 (535 bp) and KF372592 (679 bp). A BLAST search of the ITS sequence in GenBank yielded 100% nucleotide identity with the F. oxysporum (KC577181) sequence; in FUSARIUM ID [6], the TEF sequence was also identical (FD-01376). The phylogenetic trees generated using the neighbor-joining method grouped the three zucchini isolates, including JABRES2 and JABRES3, in the same clade (Figs. 2 and 3).


First Report on Fusarium Wilt of Zucchini Caused by Fusarium oxysporum, in Korea.

Choi IY, Kim JH, Lee WH, Park JH, Shin HD - Mycobiology (2015)

Phylogenetic analysis by the neighbor-joining method comparing the sequence of the internal transcribed spacer ribosomal DNA (rDNA) region from Fusarium oxysporum with that of other Fusarium spp. obtained from GenBank. The numbers above the branches represent the bootstrap value. The fungal strains identified in this study are shown in boldface.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4505008&req=5

Figure 2: Phylogenetic analysis by the neighbor-joining method comparing the sequence of the internal transcribed spacer ribosomal DNA (rDNA) region from Fusarium oxysporum with that of other Fusarium spp. obtained from GenBank. The numbers above the branches represent the bootstrap value. The fungal strains identified in this study are shown in boldface.
Mentions: The ITS and TEF sequences from a representative isolate were deposited in GenBank with the accession numbers KF372590 (535 bp) and KF372592 (679 bp). A BLAST search of the ITS sequence in GenBank yielded 100% nucleotide identity with the F. oxysporum (KC577181) sequence; in FUSARIUM ID [6], the TEF sequence was also identical (FD-01376). The phylogenetic trees generated using the neighbor-joining method grouped the three zucchini isolates, including JABRES2 and JABRES3, in the same clade (Figs. 2 and 3).

Bottom Line: Infected plants eventually died during growth.Pathogenicity of a representative isolate was demonstrated via artificial inoculation, and it satisfied Koch's postulates.To our knowledge, this is the first report of F. oxysporum causing wilt of zucchini in Korea.

View Article: PubMed Central - PubMed

Affiliation: Jeollabuk-do Agricultural Research and Extension Services, Iksan 570-704, Korea.

ABSTRACT
Fusarium wilt of zucchini in Jeonju, Korea, was first noticed in May 2013. Symptoms included wilting of the foliage, drying and withering of older leaves, and stunting of plants. Infected plants eventually died during growth. Based on morphological characteristics and phylogenetic analyses of the molecular markers (internal transcribed spacer rDNA and translation elongation factor 1α), the fungus was identified as Fusarium oxysporum. Pathogenicity of a representative isolate was demonstrated via artificial inoculation, and it satisfied Koch's postulates. To our knowledge, this is the first report of F. oxysporum causing wilt of zucchini in Korea.

No MeSH data available.