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Insecticidal Activity of Ethyl Acetate Extracts from Culture Filtrates of Mangrove Fungal Endophytes.

Abraham S, Basukriadi A, Pawiroharsono S, Sjamsuridzal W - Mycobiology (2015)

Bottom Line: The extracts used were crude extracts, so their potential as sources of AChE inhibition compounds makes them likely candidates as neurotoxins.The high-performance liquid chromatography profiles of the five extracts differed, indicating variations in their chemical constituents.This study highlights the potential of culture filtrate ethyl acetate extracts of mangrove fungal endophytes as a source of new potential bioactive compounds for bioinsecticide applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, Indonesia.

ABSTRACT
In the search for novel potent fungi-derived bioactive compounds for bioinsecticide applications, crude ethyl acetate culture filtrate extracts from 110 mangrove fungal endophytes were screened for their toxicity. Toxicity tests of all extracts against brine shrimp (Artemia salina) larvae were performed. The extracts with the highest toxicity were further examined for insecticidal activity against Spodoptera litura larvae and acetylcholinesterase (AChE) inhibition activity. The results showed that the extracts of five isolates exhibited the highest toxicity to brine shrimp at 50% lethal concentration (LC50) values of 7.45 to 10.24 ppm. These five fungal isolates that obtained from Rhizophora mucronata were identified based on sequence data analysis of the internal transcribed spacer region of rDNA as Aspergillus oryzae (strain BPPTCC 6036), Emericella nidulans (strains BPPTCC 6035 and BPPTCC 6038), A. tamarii (strain BPPTCC 6037), and A. versicolor (strain BPPTCC 6039). The mean percentage of S. litura larval mortality following topical application of the five extracts ranged from 16.7% to 43.3%. In the AChE inhibition assay, the inhibition rates of the five extracts ranged from 40.7% to 48.9%, while eserine (positive control) had an inhibition rate of 96.8%, at a concentration of 100 ppm. The extracts used were crude extracts, so their potential as sources of AChE inhibition compounds makes them likely candidates as neurotoxins. The high-performance liquid chromatography profiles of the five extracts differed, indicating variations in their chemical constituents. This study highlights the potential of culture filtrate ethyl acetate extracts of mangrove fungal endophytes as a source of new potential bioactive compounds for bioinsecticide applications.

No MeSH data available.


Related in: MedlinePlus

High-performance liquid chromatography profiles from ethyl acetate extracts of culture filtrates produced by Aspergillus oryzae BPPTCC 6036 (A), Emericella nidulans BPPTCC 6035 (B), Emericella nidulans BPPTCC 6038 (C), Aspergillus tamarii BPPTCC 6037 (D), and Aspergillus versicolor BPPTCC 6039 (E). BPPTCC, Badan Pengkajian dan Penerapan Teknologi (Agency for The Assessment and Applications of Technology) Culture Collection, Tangerang, Indonesia.
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Figure 3: High-performance liquid chromatography profiles from ethyl acetate extracts of culture filtrates produced by Aspergillus oryzae BPPTCC 6036 (A), Emericella nidulans BPPTCC 6035 (B), Emericella nidulans BPPTCC 6038 (C), Aspergillus tamarii BPPTCC 6037 (D), and Aspergillus versicolor BPPTCC 6039 (E). BPPTCC, Badan Pengkajian dan Penerapan Teknologi (Agency for The Assessment and Applications of Technology) Culture Collection, Tangerang, Indonesia.

Mentions: The HPLC profiles of the ethyl acetate extracts produced by five fungal strains (Fig. 3) show that each extract has a different peak characteristic profile. The results of the HPLC analysis of the extracts indicate that the five extracts are composed of different compounds. The profiles indicated differences between the chemical constituents and the secondary metabolites. Further studies, including fractionation, isolation, and characterization of each peak, are therefore required since each ethyl acetate extract peak might represent a single compound with a different toxicity. Additionally, assessing the toxicity degree of each fraction and elucidating the best toxic fraction may lead to the discovery of new active compounds. Through optimization of the fermentation and extraction process, the toxic fraction could be increased, consequently increasing the degree of toxicity, acute toxicity, and AChE inhibition activity of extracts.


Insecticidal Activity of Ethyl Acetate Extracts from Culture Filtrates of Mangrove Fungal Endophytes.

Abraham S, Basukriadi A, Pawiroharsono S, Sjamsuridzal W - Mycobiology (2015)

High-performance liquid chromatography profiles from ethyl acetate extracts of culture filtrates produced by Aspergillus oryzae BPPTCC 6036 (A), Emericella nidulans BPPTCC 6035 (B), Emericella nidulans BPPTCC 6038 (C), Aspergillus tamarii BPPTCC 6037 (D), and Aspergillus versicolor BPPTCC 6039 (E). BPPTCC, Badan Pengkajian dan Penerapan Teknologi (Agency for The Assessment and Applications of Technology) Culture Collection, Tangerang, Indonesia.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
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Figure 3: High-performance liquid chromatography profiles from ethyl acetate extracts of culture filtrates produced by Aspergillus oryzae BPPTCC 6036 (A), Emericella nidulans BPPTCC 6035 (B), Emericella nidulans BPPTCC 6038 (C), Aspergillus tamarii BPPTCC 6037 (D), and Aspergillus versicolor BPPTCC 6039 (E). BPPTCC, Badan Pengkajian dan Penerapan Teknologi (Agency for The Assessment and Applications of Technology) Culture Collection, Tangerang, Indonesia.
Mentions: The HPLC profiles of the ethyl acetate extracts produced by five fungal strains (Fig. 3) show that each extract has a different peak characteristic profile. The results of the HPLC analysis of the extracts indicate that the five extracts are composed of different compounds. The profiles indicated differences between the chemical constituents and the secondary metabolites. Further studies, including fractionation, isolation, and characterization of each peak, are therefore required since each ethyl acetate extract peak might represent a single compound with a different toxicity. Additionally, assessing the toxicity degree of each fraction and elucidating the best toxic fraction may lead to the discovery of new active compounds. Through optimization of the fermentation and extraction process, the toxic fraction could be increased, consequently increasing the degree of toxicity, acute toxicity, and AChE inhibition activity of extracts.

Bottom Line: The extracts used were crude extracts, so their potential as sources of AChE inhibition compounds makes them likely candidates as neurotoxins.The high-performance liquid chromatography profiles of the five extracts differed, indicating variations in their chemical constituents.This study highlights the potential of culture filtrate ethyl acetate extracts of mangrove fungal endophytes as a source of new potential bioactive compounds for bioinsecticide applications.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, Indonesia.

ABSTRACT
In the search for novel potent fungi-derived bioactive compounds for bioinsecticide applications, crude ethyl acetate culture filtrate extracts from 110 mangrove fungal endophytes were screened for their toxicity. Toxicity tests of all extracts against brine shrimp (Artemia salina) larvae were performed. The extracts with the highest toxicity were further examined for insecticidal activity against Spodoptera litura larvae and acetylcholinesterase (AChE) inhibition activity. The results showed that the extracts of five isolates exhibited the highest toxicity to brine shrimp at 50% lethal concentration (LC50) values of 7.45 to 10.24 ppm. These five fungal isolates that obtained from Rhizophora mucronata were identified based on sequence data analysis of the internal transcribed spacer region of rDNA as Aspergillus oryzae (strain BPPTCC 6036), Emericella nidulans (strains BPPTCC 6035 and BPPTCC 6038), A. tamarii (strain BPPTCC 6037), and A. versicolor (strain BPPTCC 6039). The mean percentage of S. litura larval mortality following topical application of the five extracts ranged from 16.7% to 43.3%. In the AChE inhibition assay, the inhibition rates of the five extracts ranged from 40.7% to 48.9%, while eserine (positive control) had an inhibition rate of 96.8%, at a concentration of 100 ppm. The extracts used were crude extracts, so their potential as sources of AChE inhibition compounds makes them likely candidates as neurotoxins. The high-performance liquid chromatography profiles of the five extracts differed, indicating variations in their chemical constituents. This study highlights the potential of culture filtrate ethyl acetate extracts of mangrove fungal endophytes as a source of new potential bioactive compounds for bioinsecticide applications.

No MeSH data available.


Related in: MedlinePlus