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Scytalidium parasiticum sp. nov., a New Species Parasitizing on Ganoderma boninense Isolated from Oil Palm in Peninsular Malaysia.

Goh YK, Goh TK, Marzuki NF, Tung HJ, Goh YK, Goh KJ - Mycobiology (2015)

Bottom Line: A mycoparasite, Scytalidium parasiticum sp. nov., isolated from the basidiomata of Ganoderma boninense causing basal stem rot of oil palm in Johor, Malaysia, is described and illustrated.It is distinct from other Scytalidium species in having smaller asci and ascospores (teleomorphic stage), longer arthroconidia (anamorphic stage), hyaline to yellowish chlamydospores, and producing a fluorescent pigment.A key to identify Scytalidium species with teleomorphic stage is provided.

View Article: PubMed Central - PubMed

Affiliation: Advanced Agriecological Research Sdn Bhd, 47810 Petaling Jaya, Selangor Darul Ehsan, Malaysia.

ABSTRACT
A mycoparasite, Scytalidium parasiticum sp. nov., isolated from the basidiomata of Ganoderma boninense causing basal stem rot of oil palm in Johor, Malaysia, is described and illustrated. It is distinct from other Scytalidium species in having smaller asci and ascospores (teleomorphic stage), longer arthroconidia (anamorphic stage), hyaline to yellowish chlamydospores, and producing a fluorescent pigment. The phylogenetic position of S. parasiticum was determined by sequence analyses of the internal transcribed spacers and the small-subunit ribosomal RNA gene regions. A key to identify Scytalidium species with teleomorphic stage is provided.

No MeSH data available.


Related in: MedlinePlus

Scytalidium parasiticum ex-type culture AAX0113 elucidating mature and immature ascomata (A); ornamentation on sexual fruiting body (B); ascospores from fruiting body (C); cluster of attached asci stained with lactophenol blue (D); asci stained with lactophenol blue (oil immersion objective) (E); and ascospores (F). G~H, Ascospores of S. parasiticum (arrows) (smaller in size) and S. ganodermophthorum UAMH10321 (asterisks); I, Ascospores of S. sphaerosporum UAMH10840 under oil immersion objective were included to illustrate the differences in sizes (scale bars: A = 30 µm, B~D = 10 µm, E, F = 3 µm, G~I = 5 µm).
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Figure 1: Scytalidium parasiticum ex-type culture AAX0113 elucidating mature and immature ascomata (A); ornamentation on sexual fruiting body (B); ascospores from fruiting body (C); cluster of attached asci stained with lactophenol blue (D); asci stained with lactophenol blue (oil immersion objective) (E); and ascospores (F). G~H, Ascospores of S. parasiticum (arrows) (smaller in size) and S. ganodermophthorum UAMH10321 (asterisks); I, Ascospores of S. sphaerosporum UAMH10840 under oil immersion objective were included to illustrate the differences in sizes (scale bars: A = 30 µm, B~D = 10 µm, E, F = 3 µm, G~I = 5 µm).

Mentions: Our species (isolate AAX0113) is typical of Scytalidium (Xylogone) (both the asexual and sexual stages), demonstrating the generic morphological characters such as having cleistothecial ascomata which are devoid of any appendages (Fig. 1A and 1B) and producing unicellular ascospores which are subglobose to globose, hyaline, and smooth-walled (Table 3, Fig. 1C and 1F). Ascomata of Scytalidium AAX0113 isolate (31~132 µm) were larger than those of S. sphearosporum (25~50 µm) but appeared to be comparable to those of S. ganodermophthorum (45~165 µm). Ascospores and asci (teleomorphic structures) in Scytalidium AAX0113, however, were found to be smaller when compared to those of S. ganodermophthorum and S. sphaerosporum (Table 3) (Fig. 1G~1I). When young cleistothecia were crushed, asci were seen in clusters. Each intact ascus was seen to contain 8 ascospores (Fig. 1D and 1E), but the ascal wall became evanescent at maturity. Both Scytalidium AAX0113 and S. ganodermophthorum had ascospores which were subglobose to globose, but the former were predominantly globose, with a length to width (L/l) ratio of 1.03 to 1.14. Scytalidium ganodermophthorum was having ascospores with L/l ratio of 1.08~1.10. In S. sphaerosporum however, ascospores were subglobose, with an L/l ratio of 1.60 to 1.22 (Table 3) [1521]. A conidial (asexual structures) state was also observed in Scytalidium AAX0113, morphology of which conformed to other reported species in the hyphomycete genus Scytalidium, producing tremendous amount of arthroconidia in culture. These arthroconidia appeared to be morphologically comparable to those found in Scytalidium ganodermophthorum, S. sphaerosporum, and S. cuboideum (Fig. 2A~2C) [15]. However, arthroconidia in Scytalidium AAX0113 isolate (L/l ratio of 1.0 to 3.5) were generally longer than those produced by S. ganodermophthorum (L/l ratio of 1.0 to 1.3) and S. sphaerosporum (L/1 ratio of 0.9 to 1.8) (Table 3).


Scytalidium parasiticum sp. nov., a New Species Parasitizing on Ganoderma boninense Isolated from Oil Palm in Peninsular Malaysia.

Goh YK, Goh TK, Marzuki NF, Tung HJ, Goh YK, Goh KJ - Mycobiology (2015)

Scytalidium parasiticum ex-type culture AAX0113 elucidating mature and immature ascomata (A); ornamentation on sexual fruiting body (B); ascospores from fruiting body (C); cluster of attached asci stained with lactophenol blue (D); asci stained with lactophenol blue (oil immersion objective) (E); and ascospores (F). G~H, Ascospores of S. parasiticum (arrows) (smaller in size) and S. ganodermophthorum UAMH10321 (asterisks); I, Ascospores of S. sphaerosporum UAMH10840 under oil immersion objective were included to illustrate the differences in sizes (scale bars: A = 30 µm, B~D = 10 µm, E, F = 3 µm, G~I = 5 µm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4504998&req=5

Figure 1: Scytalidium parasiticum ex-type culture AAX0113 elucidating mature and immature ascomata (A); ornamentation on sexual fruiting body (B); ascospores from fruiting body (C); cluster of attached asci stained with lactophenol blue (D); asci stained with lactophenol blue (oil immersion objective) (E); and ascospores (F). G~H, Ascospores of S. parasiticum (arrows) (smaller in size) and S. ganodermophthorum UAMH10321 (asterisks); I, Ascospores of S. sphaerosporum UAMH10840 under oil immersion objective were included to illustrate the differences in sizes (scale bars: A = 30 µm, B~D = 10 µm, E, F = 3 µm, G~I = 5 µm).
Mentions: Our species (isolate AAX0113) is typical of Scytalidium (Xylogone) (both the asexual and sexual stages), demonstrating the generic morphological characters such as having cleistothecial ascomata which are devoid of any appendages (Fig. 1A and 1B) and producing unicellular ascospores which are subglobose to globose, hyaline, and smooth-walled (Table 3, Fig. 1C and 1F). Ascomata of Scytalidium AAX0113 isolate (31~132 µm) were larger than those of S. sphearosporum (25~50 µm) but appeared to be comparable to those of S. ganodermophthorum (45~165 µm). Ascospores and asci (teleomorphic structures) in Scytalidium AAX0113, however, were found to be smaller when compared to those of S. ganodermophthorum and S. sphaerosporum (Table 3) (Fig. 1G~1I). When young cleistothecia were crushed, asci were seen in clusters. Each intact ascus was seen to contain 8 ascospores (Fig. 1D and 1E), but the ascal wall became evanescent at maturity. Both Scytalidium AAX0113 and S. ganodermophthorum had ascospores which were subglobose to globose, but the former were predominantly globose, with a length to width (L/l) ratio of 1.03 to 1.14. Scytalidium ganodermophthorum was having ascospores with L/l ratio of 1.08~1.10. In S. sphaerosporum however, ascospores were subglobose, with an L/l ratio of 1.60 to 1.22 (Table 3) [1521]. A conidial (asexual structures) state was also observed in Scytalidium AAX0113, morphology of which conformed to other reported species in the hyphomycete genus Scytalidium, producing tremendous amount of arthroconidia in culture. These arthroconidia appeared to be morphologically comparable to those found in Scytalidium ganodermophthorum, S. sphaerosporum, and S. cuboideum (Fig. 2A~2C) [15]. However, arthroconidia in Scytalidium AAX0113 isolate (L/l ratio of 1.0 to 3.5) were generally longer than those produced by S. ganodermophthorum (L/l ratio of 1.0 to 1.3) and S. sphaerosporum (L/1 ratio of 0.9 to 1.8) (Table 3).

Bottom Line: A mycoparasite, Scytalidium parasiticum sp. nov., isolated from the basidiomata of Ganoderma boninense causing basal stem rot of oil palm in Johor, Malaysia, is described and illustrated.It is distinct from other Scytalidium species in having smaller asci and ascospores (teleomorphic stage), longer arthroconidia (anamorphic stage), hyaline to yellowish chlamydospores, and producing a fluorescent pigment.A key to identify Scytalidium species with teleomorphic stage is provided.

View Article: PubMed Central - PubMed

Affiliation: Advanced Agriecological Research Sdn Bhd, 47810 Petaling Jaya, Selangor Darul Ehsan, Malaysia.

ABSTRACT
A mycoparasite, Scytalidium parasiticum sp. nov., isolated from the basidiomata of Ganoderma boninense causing basal stem rot of oil palm in Johor, Malaysia, is described and illustrated. It is distinct from other Scytalidium species in having smaller asci and ascospores (teleomorphic stage), longer arthroconidia (anamorphic stage), hyaline to yellowish chlamydospores, and producing a fluorescent pigment. The phylogenetic position of S. parasiticum was determined by sequence analyses of the internal transcribed spacers and the small-subunit ribosomal RNA gene regions. A key to identify Scytalidium species with teleomorphic stage is provided.

No MeSH data available.


Related in: MedlinePlus