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High ERα36 Expression Level and Membrane Location Predict Poor Prognosis in Renal Cell Carcinoma.

Wang Q, Zhang W, Yang J, Liu YL, Yan ZX, Guo ZJ, Li YJ, Bian XW - Medicine (Baltimore) (2015)

Bottom Line: The differential diagnosis value was also assessed for benign and malignant renal tumors, as well as its prognostic value.The results showed that membrane ERα36 expression was rarely detected in benign tumors but predominantly observed in malignant renal tumors.Kaplan-Meier analysis indicated that significant correlations of high ERα36 level and ERα36 membrane expression were correlated with both poor disease-free survival and overall survival.Both ERα36 high expression and membrane location indicate poor prognosis in renal cell carcinoma.

View Article: PubMed Central - PubMed

Affiliation: From the Institute of Pathology and Southwest Cancer Center (QW, JY, Z-XY, Z-JG, X-WB), Southwest Hospital, Third Military Medical University, Chongqing; Department of Pathology (QW, WZ); Department of Clinical Laboratory (Y-LL), The 401st People's Liberation Army Hospital; and Department of Pathology (Y-JL), Affiliated Hospital of Medical College, Qingdao University, Qingdao, China.

ABSTRACT
Estrogen receptor alpha 36 (ERα36), a truncated variant of ERα, is located in cytoplasm and membrane that is different from other nuclear receptors of ERα family. ERα36 is involved in progression and treatment resistance of a variety of carcinomas. However, the clinical and prognostic significance of ERα36 in renal tumors have not been fully elucidated.Here, renal tumor tissues from 125 patients were collected and immunohistochemical stained with ERα36 antibody. ERα36 expression level and location in these cases were analyzed for their correlations with clinical characteristics. The differential diagnosis value was also assessed for benign and malignant renal tumors, as well as its prognostic value.The results showed that membrane ERα36 expression was rarely detected in benign tumors but predominantly observed in malignant renal tumors. Kaplan-Meier analysis indicated that significant correlations of high ERα36 level and ERα36 membrane expression were correlated with both poor disease-free survival and overall survival. Univariate and multivariate analysis confirmed that both ERα36 high expression and membrane location can serve as unfavorable prognostic indicators for renal cell carcinoma.It is thus concluded that membrane ERα36 expression is valuable for differential diagnosis of malignant renal tumors from benign ones. Both ERα36 high expression and membrane location indicate poor prognosis in renal cell carcinoma.

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Related in: MedlinePlus

ERα36 expression in renal tumors (immunohistochemistry). (A, B) Low immunoreactivity was observed in the pericarcinous renal tissues: nephron (A) and renal tubules (B). (C, D) Most benign renal tumors showed dominant cytoplasm ERα36 expression (C). Only 1 case showed weak membrane location (D). (E, F) ERα36 positive staining was observed in the membrane (E) or cytoplasm (F) of renal cell carcinomas. Representative tumor cells positive for cytoplasm or membrane were shown with arrows (green arrows, cytoplasm; red arrows, membrane). Scale bar = 50 μm. ERα36 = estrogen receptor alpha 36.
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Figure 1: ERα36 expression in renal tumors (immunohistochemistry). (A, B) Low immunoreactivity was observed in the pericarcinous renal tissues: nephron (A) and renal tubules (B). (C, D) Most benign renal tumors showed dominant cytoplasm ERα36 expression (C). Only 1 case showed weak membrane location (D). (E, F) ERα36 positive staining was observed in the membrane (E) or cytoplasm (F) of renal cell carcinomas. Representative tumor cells positive for cytoplasm or membrane were shown with arrows (green arrows, cytoplasm; red arrows, membrane). Scale bar = 50 μm. ERα36 = estrogen receptor alpha 36.

Mentions: A total of 99 patients with renal cell carcinoma were analyzed for ERα36 expression, as well as another 26 cases of diagnosed benign renal tumor. Immunohistochemical staining showed that the pericarcinous renal tissues were observed with low ERα36 immunoreactivity. ERα36 expression was rarely observed in nephron (Figure 1A), but found in some renal tubules (Figure 1B). However, ERα36 expression was found in benign renal tumors (Figure 1C, D). High ERα36 expression was also observed in primary renal cell carcinoma, which was predominantly located in the cytoplasm and membrane of cancer cells (Figure 1E, F). In the cancer cell bulks, ERα36 expression was distributed primarily in a hierarchical pattern (Figure 1F).


High ERα36 Expression Level and Membrane Location Predict Poor Prognosis in Renal Cell Carcinoma.

Wang Q, Zhang W, Yang J, Liu YL, Yan ZX, Guo ZJ, Li YJ, Bian XW - Medicine (Baltimore) (2015)

ERα36 expression in renal tumors (immunohistochemistry). (A, B) Low immunoreactivity was observed in the pericarcinous renal tissues: nephron (A) and renal tubules (B). (C, D) Most benign renal tumors showed dominant cytoplasm ERα36 expression (C). Only 1 case showed weak membrane location (D). (E, F) ERα36 positive staining was observed in the membrane (E) or cytoplasm (F) of renal cell carcinomas. Representative tumor cells positive for cytoplasm or membrane were shown with arrows (green arrows, cytoplasm; red arrows, membrane). Scale bar = 50 μm. ERα36 = estrogen receptor alpha 36.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4504609&req=5

Figure 1: ERα36 expression in renal tumors (immunohistochemistry). (A, B) Low immunoreactivity was observed in the pericarcinous renal tissues: nephron (A) and renal tubules (B). (C, D) Most benign renal tumors showed dominant cytoplasm ERα36 expression (C). Only 1 case showed weak membrane location (D). (E, F) ERα36 positive staining was observed in the membrane (E) or cytoplasm (F) of renal cell carcinomas. Representative tumor cells positive for cytoplasm or membrane were shown with arrows (green arrows, cytoplasm; red arrows, membrane). Scale bar = 50 μm. ERα36 = estrogen receptor alpha 36.
Mentions: A total of 99 patients with renal cell carcinoma were analyzed for ERα36 expression, as well as another 26 cases of diagnosed benign renal tumor. Immunohistochemical staining showed that the pericarcinous renal tissues were observed with low ERα36 immunoreactivity. ERα36 expression was rarely observed in nephron (Figure 1A), but found in some renal tubules (Figure 1B). However, ERα36 expression was found in benign renal tumors (Figure 1C, D). High ERα36 expression was also observed in primary renal cell carcinoma, which was predominantly located in the cytoplasm and membrane of cancer cells (Figure 1E, F). In the cancer cell bulks, ERα36 expression was distributed primarily in a hierarchical pattern (Figure 1F).

Bottom Line: The differential diagnosis value was also assessed for benign and malignant renal tumors, as well as its prognostic value.The results showed that membrane ERα36 expression was rarely detected in benign tumors but predominantly observed in malignant renal tumors.Kaplan-Meier analysis indicated that significant correlations of high ERα36 level and ERα36 membrane expression were correlated with both poor disease-free survival and overall survival.Both ERα36 high expression and membrane location indicate poor prognosis in renal cell carcinoma.

View Article: PubMed Central - PubMed

Affiliation: From the Institute of Pathology and Southwest Cancer Center (QW, JY, Z-XY, Z-JG, X-WB), Southwest Hospital, Third Military Medical University, Chongqing; Department of Pathology (QW, WZ); Department of Clinical Laboratory (Y-LL), The 401st People's Liberation Army Hospital; and Department of Pathology (Y-JL), Affiliated Hospital of Medical College, Qingdao University, Qingdao, China.

ABSTRACT
Estrogen receptor alpha 36 (ERα36), a truncated variant of ERα, is located in cytoplasm and membrane that is different from other nuclear receptors of ERα family. ERα36 is involved in progression and treatment resistance of a variety of carcinomas. However, the clinical and prognostic significance of ERα36 in renal tumors have not been fully elucidated.Here, renal tumor tissues from 125 patients were collected and immunohistochemical stained with ERα36 antibody. ERα36 expression level and location in these cases were analyzed for their correlations with clinical characteristics. The differential diagnosis value was also assessed for benign and malignant renal tumors, as well as its prognostic value.The results showed that membrane ERα36 expression was rarely detected in benign tumors but predominantly observed in malignant renal tumors. Kaplan-Meier analysis indicated that significant correlations of high ERα36 level and ERα36 membrane expression were correlated with both poor disease-free survival and overall survival. Univariate and multivariate analysis confirmed that both ERα36 high expression and membrane location can serve as unfavorable prognostic indicators for renal cell carcinoma.It is thus concluded that membrane ERα36 expression is valuable for differential diagnosis of malignant renal tumors from benign ones. Both ERα36 high expression and membrane location indicate poor prognosis in renal cell carcinoma.

Show MeSH
Related in: MedlinePlus