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Microarray Analysis Reveals Potential Biological Functions of Histone H2B Monoubiquitination.

Wu Y, Chen P, Jing Y, Wang C, Men YL, Zhan W, Wang Q, Gan Z, Huang J, Xie K, Mi J, Yu C, Yu X, Chen PC, Chang JF, Cai F, Chen S - PLoS ONE (2015)

Bottom Line: Histone H2B monoubiquitination is a key histone modification that has significant effects on chromatin higher-order structure and gene transcription.Bioinformatics analysis of our data demonstrated that while H2B monoubiquitination expectedly affected a number of previously reported biological pathways, we also uncovered the influence of this histone modification on many novel biological processes.Therefore, our work provided valuable information for understanding the role of H2B monoubiquitination and indicated potential directions for its further studies.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Department of Breast Surgery of Yangpu Hospital, Tongji University, Shanghai 200092, P. R. China.

ABSTRACT
Histone H2B monoubiquitination is a key histone modification that has significant effects on chromatin higher-order structure and gene transcription. Multiple biological processes have been suggested to be tightly related to the dynamics of H2B monoubiquitination. However, a comprehensive understanding of biological roles of H2B monoubiquitination is still poorly understood. In the present study, we developed an efficient tool to disrupt endogenous H2B monoubiquitination levels by using an H2BK120R mutant construct expressed in human cells. Genome-wide microarray analysis of these cells revealed a potential global view of biological functions of H2B monoubiquitination. Bioinformatics analysis of our data demonstrated that while H2B monoubiquitination expectedly affected a number of previously reported biological pathways, we also uncovered the influence of this histone modification on many novel biological processes. Therefore, our work provided valuable information for understanding the role of H2B monoubiquitination and indicated potential directions for its further studies.

No MeSH data available.


Correlation between genes differentially expressed following RNF20 knockdown and overexpression of H2BK120R.Gene correlation analysis was performed with the online MAS 3.0 software (a molecule annotation system, http://bioinfo.capitalbio.com/mas3/).
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pone.0133444.g003: Correlation between genes differentially expressed following RNF20 knockdown and overexpression of H2BK120R.Gene correlation analysis was performed with the online MAS 3.0 software (a molecule annotation system, http://bioinfo.capitalbio.com/mas3/).

Mentions: To understand global functional relationships between differentially expressed genes, gene correlation assay was performed using online MAS 3.0 software (Molecule Annotation System, http://bioinfo.capitalbio.com/mas3/). The results showed that many differentially expressed genes are strongly associated with the EGFR (epidermal growth factor receptor)-related signaling (Fig 3).


Microarray Analysis Reveals Potential Biological Functions of Histone H2B Monoubiquitination.

Wu Y, Chen P, Jing Y, Wang C, Men YL, Zhan W, Wang Q, Gan Z, Huang J, Xie K, Mi J, Yu C, Yu X, Chen PC, Chang JF, Cai F, Chen S - PLoS ONE (2015)

Correlation between genes differentially expressed following RNF20 knockdown and overexpression of H2BK120R.Gene correlation analysis was performed with the online MAS 3.0 software (a molecule annotation system, http://bioinfo.capitalbio.com/mas3/).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4503354&req=5

pone.0133444.g003: Correlation between genes differentially expressed following RNF20 knockdown and overexpression of H2BK120R.Gene correlation analysis was performed with the online MAS 3.0 software (a molecule annotation system, http://bioinfo.capitalbio.com/mas3/).
Mentions: To understand global functional relationships between differentially expressed genes, gene correlation assay was performed using online MAS 3.0 software (Molecule Annotation System, http://bioinfo.capitalbio.com/mas3/). The results showed that many differentially expressed genes are strongly associated with the EGFR (epidermal growth factor receptor)-related signaling (Fig 3).

Bottom Line: Histone H2B monoubiquitination is a key histone modification that has significant effects on chromatin higher-order structure and gene transcription.Bioinformatics analysis of our data demonstrated that while H2B monoubiquitination expectedly affected a number of previously reported biological pathways, we also uncovered the influence of this histone modification on many novel biological processes.Therefore, our work provided valuable information for understanding the role of H2B monoubiquitination and indicated potential directions for its further studies.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Department of Breast Surgery of Yangpu Hospital, Tongji University, Shanghai 200092, P. R. China.

ABSTRACT
Histone H2B monoubiquitination is a key histone modification that has significant effects on chromatin higher-order structure and gene transcription. Multiple biological processes have been suggested to be tightly related to the dynamics of H2B monoubiquitination. However, a comprehensive understanding of biological roles of H2B monoubiquitination is still poorly understood. In the present study, we developed an efficient tool to disrupt endogenous H2B monoubiquitination levels by using an H2BK120R mutant construct expressed in human cells. Genome-wide microarray analysis of these cells revealed a potential global view of biological functions of H2B monoubiquitination. Bioinformatics analysis of our data demonstrated that while H2B monoubiquitination expectedly affected a number of previously reported biological pathways, we also uncovered the influence of this histone modification on many novel biological processes. Therefore, our work provided valuable information for understanding the role of H2B monoubiquitination and indicated potential directions for its further studies.

No MeSH data available.