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Effect of microfracture and autologous-conditioned plasma application in the focal full-thickness chondral defect of the knee: an experimental study on rabbits.

Karakaplan M, Elmalı N, Mirel E, Şahin N, Ergen E, Elmalı C - J Orthop Surg Res (2015)

Bottom Line: Findings showed that in both mfx/ACP-treated groups, the defects were filled regularly and smoothly, the defects had a greater fill and good integration into the surrounding host tissue, and the repair matrix had more hyaline-like character.On the other hand, defects were filled with an irregular, fibrous cartilage in the mfx-treated group.Histological scores in Group 2 and Group 3 were better compared to Group 1.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedics and Traumatology Department, Turgut Ozal Medical Center, Inonu University Medical School, Malatya, 44100, Turkey.

ABSTRACT

Purpose: The aim of the present study was to evaluate the effect of microfracture and intraarticular autologous conditioned plasma (ACP) injection on cartilage regeneration in a focal full-thickness chondral defect model created in the knee joint.

Methods: Full-thickness chondral defects of 3 × 6 mm(2) were surgically created in right medial femoral condyles (MFC) of New Zealand rabbits, and the rabbits were then divided into three groups according to treatment: Group 1 received only microfracture (mfx), Group 2 received mfx plus intraarticular ACP, and Group 3 received mfx; the defect was covered by the periosteum, and then, ACP was applied subperiosteally and intraarticularly. Twelve weeks after injection, the animals were sacrificed and the femoral condyles were evaluated macroscopically and histologically by hematoxylin-eosin staining. Then, histological sections were scored using the International Cartilage Repair Society (ICRS) visual histological scale.

Results: Findings showed that in both mfx/ACP-treated groups, the defects were filled regularly and smoothly, the defects had a greater fill and good integration into the surrounding host tissue, and the repair matrix had more hyaline-like character. On the other hand, defects were filled with an irregular, fibrous cartilage in the mfx-treated group. Histological scores in Group 2 and Group 3 were better compared to Group 1.

Conclusion: In the present study, we were able to demonstrate a beneficial effect of intraarticular administration of ACP as a coadjuvant of microfractures in order to regenerate hyaline-like cartilage in full-thickness chondral lesions in a rabbit model.

No MeSH data available.


Related in: MedlinePlus

In the mfx plus intraarticular ACP treated group (Group 2), there are more regular surface (arrows), hyaline matrix mixed with fibrocartilage, in cell distribution criteria, disorganized cell distribution (asterisks) (H&E)
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Fig5: In the mfx plus intraarticular ACP treated group (Group 2), there are more regular surface (arrows), hyaline matrix mixed with fibrocartilage, in cell distribution criteria, disorganized cell distribution (asterisks) (H&E)

Mentions: In the mfx-treated group (group 1), all of the samples presented an irregular cartilage surface, a surface with a fibrocartilage matrix, and predominantly disorganized cell distribution (Fig. 4). The mfx/ACP-treated group (Group 2) showed mainly a more regular surface, hyaline matrix mixed with fibrocartilage, and differences in cell distribution criteria, ranging from samples with a disorganized cell distribution to others with mixed columnar to cluster distribution (Fig. 5). In Group 3, the tissue surface was regular, with a hyaline matrix mixed with fibrocartilage in some areas but with chondrocytes more organized in the tissue compared to the mfx/ACP-treated group (Fig. 6). Cartilage mineralization was unaltered in all groups. When compared with both mfx/ACP-treated groups, the mfx group showed a significant reduction in scores in all categories (Table 3, P < 0.05). No difference was found in the interobserver histological grading.Fig. 4


Effect of microfracture and autologous-conditioned plasma application in the focal full-thickness chondral defect of the knee: an experimental study on rabbits.

Karakaplan M, Elmalı N, Mirel E, Şahin N, Ergen E, Elmalı C - J Orthop Surg Res (2015)

In the mfx plus intraarticular ACP treated group (Group 2), there are more regular surface (arrows), hyaline matrix mixed with fibrocartilage, in cell distribution criteria, disorganized cell distribution (asterisks) (H&E)
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4502561&req=5

Fig5: In the mfx plus intraarticular ACP treated group (Group 2), there are more regular surface (arrows), hyaline matrix mixed with fibrocartilage, in cell distribution criteria, disorganized cell distribution (asterisks) (H&E)
Mentions: In the mfx-treated group (group 1), all of the samples presented an irregular cartilage surface, a surface with a fibrocartilage matrix, and predominantly disorganized cell distribution (Fig. 4). The mfx/ACP-treated group (Group 2) showed mainly a more regular surface, hyaline matrix mixed with fibrocartilage, and differences in cell distribution criteria, ranging from samples with a disorganized cell distribution to others with mixed columnar to cluster distribution (Fig. 5). In Group 3, the tissue surface was regular, with a hyaline matrix mixed with fibrocartilage in some areas but with chondrocytes more organized in the tissue compared to the mfx/ACP-treated group (Fig. 6). Cartilage mineralization was unaltered in all groups. When compared with both mfx/ACP-treated groups, the mfx group showed a significant reduction in scores in all categories (Table 3, P < 0.05). No difference was found in the interobserver histological grading.Fig. 4

Bottom Line: Findings showed that in both mfx/ACP-treated groups, the defects were filled regularly and smoothly, the defects had a greater fill and good integration into the surrounding host tissue, and the repair matrix had more hyaline-like character.On the other hand, defects were filled with an irregular, fibrous cartilage in the mfx-treated group.Histological scores in Group 2 and Group 3 were better compared to Group 1.

View Article: PubMed Central - PubMed

Affiliation: Orthopaedics and Traumatology Department, Turgut Ozal Medical Center, Inonu University Medical School, Malatya, 44100, Turkey.

ABSTRACT

Purpose: The aim of the present study was to evaluate the effect of microfracture and intraarticular autologous conditioned plasma (ACP) injection on cartilage regeneration in a focal full-thickness chondral defect model created in the knee joint.

Methods: Full-thickness chondral defects of 3 × 6 mm(2) were surgically created in right medial femoral condyles (MFC) of New Zealand rabbits, and the rabbits were then divided into three groups according to treatment: Group 1 received only microfracture (mfx), Group 2 received mfx plus intraarticular ACP, and Group 3 received mfx; the defect was covered by the periosteum, and then, ACP was applied subperiosteally and intraarticularly. Twelve weeks after injection, the animals were sacrificed and the femoral condyles were evaluated macroscopically and histologically by hematoxylin-eosin staining. Then, histological sections were scored using the International Cartilage Repair Society (ICRS) visual histological scale.

Results: Findings showed that in both mfx/ACP-treated groups, the defects were filled regularly and smoothly, the defects had a greater fill and good integration into the surrounding host tissue, and the repair matrix had more hyaline-like character. On the other hand, defects were filled with an irregular, fibrous cartilage in the mfx-treated group. Histological scores in Group 2 and Group 3 were better compared to Group 1.

Conclusion: In the present study, we were able to demonstrate a beneficial effect of intraarticular administration of ACP as a coadjuvant of microfractures in order to regenerate hyaline-like cartilage in full-thickness chondral lesions in a rabbit model.

No MeSH data available.


Related in: MedlinePlus