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Evaluation of a water extract of So-Cheong-Ryong-Tang for acute toxicity and genotoxicity using in vitro and in vivo tests.

Lee MY, Seo CS, Kim JY, Shin HK - BMC Complement Altern Med (2015)

Bottom Line: However, its genotoxicity has been rarely examined.In the bacterial reverse mutation assay, SCRT did not increase revertant colony numbers in Salmonella. typhimurium strains (TA98, TA100, TA1535, and TA1537) or an Escherichia coli strain (WP2 uvrA) regardless of metabolic activation or the duration of treatment.However, statistically significant differences in the incidence of chromosomal aberrations following SCRT>4000 μg/mL were observed in Chinese hamster lung cells exposed with or without an S9 enzyme and cofactor mixture.

View Article: PubMed Central - PubMed

Affiliation: Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine, 483 Expo-ro, Yusung-gu, Daejeon, 305-811, South Korea. cozy37@gmail.com.

ABSTRACT

Background: So-Cheong-Ryong-Tang, a traditional Korean medicine, has been used empirically for the treatment of asthma, allergic rhinitis, and colds for hundreds of years. However, its genotoxicity has been rarely examined.

Methods: We therefore investigated the genotoxicity of an aqueous extract of So-Cheong-Ryong-Tang (SCRT) in two in vitro and one in vivo assays: a bacterial reverse mutation assay (Ames test), a chromosomal aberration assay, and a micronucleus assay, respectively.

Results: In the bacterial reverse mutation assay, SCRT did not increase revertant colony numbers in Salmonella. typhimurium strains (TA98, TA100, TA1535, and TA1537) or an Escherichia coli strain (WP2 uvrA) regardless of metabolic activation or the duration of treatment. However, statistically significant differences in the incidence of chromosomal aberrations following SCRT>4000 μg/mL were observed in Chinese hamster lung cells exposed with or without an S9 enzyme and cofactor mixture.

Conclusions: These results suggest further genotoxic testing of SCRT, such as a comet assay, to ascertain its generally recognized safety.

No MeSH data available.


Related in: MedlinePlus

Effect of SCRT on bacterial reverse mutation assay (Ames test) (a) with (+S9 mix) and (b) without (−S9 mix) metabolic activation. BP: Benzo(a)pyrene, SA: Sodium azide, 2-AA: 2-Aminoanthracene, 2-NF: 2-Nitrofluorene, 4NQO: 4-Nitroquinoline N-oxide, 9-AA: 9-Aminoacridine, VC: vehicle control
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Fig4: Effect of SCRT on bacterial reverse mutation assay (Ames test) (a) with (+S9 mix) and (b) without (−S9 mix) metabolic activation. BP: Benzo(a)pyrene, SA: Sodium azide, 2-AA: 2-Aminoanthracene, 2-NF: 2-Nitrofluorene, 4NQO: 4-Nitroquinoline N-oxide, 9-AA: 9-Aminoacridine, VC: vehicle control

Mentions: The positive controls showed significantly increased numbers of revertant colonies, indicating that the assay was valid. No positive mutagenic response was observed in any of the S. typhimurium or E. coli strains tested compared with the concurrent vehicle control groups regardless of the presence (Fig. 4a) or absence (Fig. 4b) of the S9 mixture up to 5000 μg/plate.Fig. 4


Evaluation of a water extract of So-Cheong-Ryong-Tang for acute toxicity and genotoxicity using in vitro and in vivo tests.

Lee MY, Seo CS, Kim JY, Shin HK - BMC Complement Altern Med (2015)

Effect of SCRT on bacterial reverse mutation assay (Ames test) (a) with (+S9 mix) and (b) without (−S9 mix) metabolic activation. BP: Benzo(a)pyrene, SA: Sodium azide, 2-AA: 2-Aminoanthracene, 2-NF: 2-Nitrofluorene, 4NQO: 4-Nitroquinoline N-oxide, 9-AA: 9-Aminoacridine, VC: vehicle control
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4502466&req=5

Fig4: Effect of SCRT on bacterial reverse mutation assay (Ames test) (a) with (+S9 mix) and (b) without (−S9 mix) metabolic activation. BP: Benzo(a)pyrene, SA: Sodium azide, 2-AA: 2-Aminoanthracene, 2-NF: 2-Nitrofluorene, 4NQO: 4-Nitroquinoline N-oxide, 9-AA: 9-Aminoacridine, VC: vehicle control
Mentions: The positive controls showed significantly increased numbers of revertant colonies, indicating that the assay was valid. No positive mutagenic response was observed in any of the S. typhimurium or E. coli strains tested compared with the concurrent vehicle control groups regardless of the presence (Fig. 4a) or absence (Fig. 4b) of the S9 mixture up to 5000 μg/plate.Fig. 4

Bottom Line: However, its genotoxicity has been rarely examined.In the bacterial reverse mutation assay, SCRT did not increase revertant colony numbers in Salmonella. typhimurium strains (TA98, TA100, TA1535, and TA1537) or an Escherichia coli strain (WP2 uvrA) regardless of metabolic activation or the duration of treatment.However, statistically significant differences in the incidence of chromosomal aberrations following SCRT>4000 μg/mL were observed in Chinese hamster lung cells exposed with or without an S9 enzyme and cofactor mixture.

View Article: PubMed Central - PubMed

Affiliation: Herbal Medicine Formulation Research Group, Korea Institute of Oriental Medicine, 483 Expo-ro, Yusung-gu, Daejeon, 305-811, South Korea. cozy37@gmail.com.

ABSTRACT

Background: So-Cheong-Ryong-Tang, a traditional Korean medicine, has been used empirically for the treatment of asthma, allergic rhinitis, and colds for hundreds of years. However, its genotoxicity has been rarely examined.

Methods: We therefore investigated the genotoxicity of an aqueous extract of So-Cheong-Ryong-Tang (SCRT) in two in vitro and one in vivo assays: a bacterial reverse mutation assay (Ames test), a chromosomal aberration assay, and a micronucleus assay, respectively.

Results: In the bacterial reverse mutation assay, SCRT did not increase revertant colony numbers in Salmonella. typhimurium strains (TA98, TA100, TA1535, and TA1537) or an Escherichia coli strain (WP2 uvrA) regardless of metabolic activation or the duration of treatment. However, statistically significant differences in the incidence of chromosomal aberrations following SCRT>4000 μg/mL were observed in Chinese hamster lung cells exposed with or without an S9 enzyme and cofactor mixture.

Conclusions: These results suggest further genotoxic testing of SCRT, such as a comet assay, to ascertain its generally recognized safety.

No MeSH data available.


Related in: MedlinePlus