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The Drosophila Prosecretory Transcription Factor dimmed Is Dynamically Regulated in Adult Enteroendocrine Cells and Protects Against Gram-Negative Infection.

Beebe K, Park D, Taghert PH, Micchelli CA - G3 (Bethesda) (2015)

Bottom Line: However, it remains unclear how this sensory epithelium responds to specific pathogenic challenges in a dynamic and regulated manner.We demonstrate that the enteroendocrine cells of the adult Drosophila melanogaster midgut display a transient, sensitive, and systemic induction of the prosecretory factor dimmed (dimm) in response to the Gram-negative pathogen Pseudomonas entomophila (Pe).We propose that dimm provides "gain" in enteroendocrine output during the adaptive response to episodic pathogen exposure.

View Article: PubMed Central - PubMed

Affiliation: Department of Developmental Biology, Washington University School of Medicine, St. Louis, Missouri 63110.

No MeSH data available.


Related in: MedlinePlus

dimm is a host factor that protects against Pseudomonas entomophila (Pe) infection. Survival and immune response of dimm mutants after Gram-negative Pe infection. (A) Survival of wild-type and dimm mutants after exposure to mock or Pe treatment. Flies were exposed to Pe OD 10 for 24 hr (n = 4 trials, 80 females). (B) Quantitative polymerase chain reaction analysis of mRNA in whole-body tissue of a subset of antimicrobial peptides in wild-type and dimm mutant genotypes, Pe OD 5, collected 24 hr after Pe (wild-type, black bars; dimm mutant, gray bars). Fold change represents Pe compared with mock using the 2−ΔΔCT method (n = 3 trials, 30 flies). Bars indicate mean values ± SEM.
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fig5: dimm is a host factor that protects against Pseudomonas entomophila (Pe) infection. Survival and immune response of dimm mutants after Gram-negative Pe infection. (A) Survival of wild-type and dimm mutants after exposure to mock or Pe treatment. Flies were exposed to Pe OD 10 for 24 hr (n = 4 trials, 80 females). (B) Quantitative polymerase chain reaction analysis of mRNA in whole-body tissue of a subset of antimicrobial peptides in wild-type and dimm mutant genotypes, Pe OD 5, collected 24 hr after Pe (wild-type, black bars; dimm mutant, gray bars). Fold change represents Pe compared with mock using the 2−ΔΔCT method (n = 3 trials, 30 flies). Bars indicate mean values ± SEM.

Mentions: Log-rank (Mantel-Cox) test was performed. Figure 1G and Table S2: n = 4 trials, 80 females. Figure 5A and Table S3: n = 4 trials, 80 females.


The Drosophila Prosecretory Transcription Factor dimmed Is Dynamically Regulated in Adult Enteroendocrine Cells and Protects Against Gram-Negative Infection.

Beebe K, Park D, Taghert PH, Micchelli CA - G3 (Bethesda) (2015)

dimm is a host factor that protects against Pseudomonas entomophila (Pe) infection. Survival and immune response of dimm mutants after Gram-negative Pe infection. (A) Survival of wild-type and dimm mutants after exposure to mock or Pe treatment. Flies were exposed to Pe OD 10 for 24 hr (n = 4 trials, 80 females). (B) Quantitative polymerase chain reaction analysis of mRNA in whole-body tissue of a subset of antimicrobial peptides in wild-type and dimm mutant genotypes, Pe OD 5, collected 24 hr after Pe (wild-type, black bars; dimm mutant, gray bars). Fold change represents Pe compared with mock using the 2−ΔΔCT method (n = 3 trials, 30 flies). Bars indicate mean values ± SEM.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4502385&req=5

fig5: dimm is a host factor that protects against Pseudomonas entomophila (Pe) infection. Survival and immune response of dimm mutants after Gram-negative Pe infection. (A) Survival of wild-type and dimm mutants after exposure to mock or Pe treatment. Flies were exposed to Pe OD 10 for 24 hr (n = 4 trials, 80 females). (B) Quantitative polymerase chain reaction analysis of mRNA in whole-body tissue of a subset of antimicrobial peptides in wild-type and dimm mutant genotypes, Pe OD 5, collected 24 hr after Pe (wild-type, black bars; dimm mutant, gray bars). Fold change represents Pe compared with mock using the 2−ΔΔCT method (n = 3 trials, 30 flies). Bars indicate mean values ± SEM.
Mentions: Log-rank (Mantel-Cox) test was performed. Figure 1G and Table S2: n = 4 trials, 80 females. Figure 5A and Table S3: n = 4 trials, 80 females.

Bottom Line: However, it remains unclear how this sensory epithelium responds to specific pathogenic challenges in a dynamic and regulated manner.We demonstrate that the enteroendocrine cells of the adult Drosophila melanogaster midgut display a transient, sensitive, and systemic induction of the prosecretory factor dimmed (dimm) in response to the Gram-negative pathogen Pseudomonas entomophila (Pe).We propose that dimm provides "gain" in enteroendocrine output during the adaptive response to episodic pathogen exposure.

View Article: PubMed Central - PubMed

Affiliation: Department of Developmental Biology, Washington University School of Medicine, St. Louis, Missouri 63110.

No MeSH data available.


Related in: MedlinePlus