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RAD-QTL Mapping Reveals Both Genome-Level Parallelism and Different Genetic Architecture Underlying the Evolution of Body Shape in Lake Whitefish (Coregonus clupeaformis) Species Pairs.

Laporte M, Rogers SM, Dion-Côté AM, Normandeau E, Gagnaire PA, Dalziel AC, Chebib J, Bernatchez L - G3 (Bethesda) (2015)

Bottom Line: Using a genetic map comprising 3438 restriction site-associated DNA sequencing single-nucleotide polymorphisms, we then identified quantitative trait loci underlying body shape traits in a backcross family reared in the laboratory.The single-locus approach provided limited evidence for genetic parallelism.These results provide evidence for both genetic parallelism and multiple genetic routes underlying parallel phenotypic evolution in fish shape among populations occupying similar ecological niches.

View Article: PubMed Central - PubMed

Affiliation: Institut de Biologie Intégrative et des Systèmes (IBIS), Département de Biologie, Université Laval, Pavillon Charles-Eugène-Marchand, Québec, G1V 0A6, Canada uni.mlaporte@gmail.com.

No MeSH data available.


Related in: MedlinePlus

Mean shape comparisons between normal (white dot; dashed line) and dwarf Whitefish (black dot; full line) for Cliff, East, Indian, Témiscouata, and Webster lakes. Red arrows show how shape changes from normal to dwarf Whitefish.
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fig2: Mean shape comparisons between normal (white dot; dashed line) and dwarf Whitefish (black dot; full line) for Cliff, East, Indian, Témiscouata, and Webster lakes. Red arrows show how shape changes from normal to dwarf Whitefish.

Mentions: Broken-stick distribution indicated that only the two first PCs were informative. The first axis represents 50.6% of the variation and show variation among lakes (Figure 2). With the exception of Témiscouata Lake, dwarf Whitefish species also showed lower mean score on this axis than normal Whitefish (Figure 2). The second axis represents 35.7% of the variation and showed a clear distinction between all dwarf and normal Whitefish (Figure 2). Euclidian distance showed an increasing gradient in the following order: Webster (0.020; P-value = 0.032), Témiscouata (0.021; P-value < 0.001), Cliff (0.028; P-value < 0.001), East (0.032; P-value < 0.001), and Indian (0.038; P-value < 0.001). This gradient roughly correspond to the one observed with the juxtaposition of mean shape (Figure 3). In addition, all between-group PCA angles point in a similar direction (9−55°). Interestingly, the lower angle observed (Cliff: 9°) is linked to a ventral thinning in dwarf in comparison to normal Whitefish (Figure 3), the greater angle (Témiscouata: 55°) is linked to a dorsal thinning in dwarf (Figure 3) and the intermediary angles (Indian: 18°, Webster: 22°, and East: 25°) are linked to both ventral and dorsal thinning (Figure 3). Dwarf Whitefish also have bigger eyes and longer tails than the normal fish in all of the five lakes (Figure 3).


RAD-QTL Mapping Reveals Both Genome-Level Parallelism and Different Genetic Architecture Underlying the Evolution of Body Shape in Lake Whitefish (Coregonus clupeaformis) Species Pairs.

Laporte M, Rogers SM, Dion-Côté AM, Normandeau E, Gagnaire PA, Dalziel AC, Chebib J, Bernatchez L - G3 (Bethesda) (2015)

Mean shape comparisons between normal (white dot; dashed line) and dwarf Whitefish (black dot; full line) for Cliff, East, Indian, Témiscouata, and Webster lakes. Red arrows show how shape changes from normal to dwarf Whitefish.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4502382&req=5

fig2: Mean shape comparisons between normal (white dot; dashed line) and dwarf Whitefish (black dot; full line) for Cliff, East, Indian, Témiscouata, and Webster lakes. Red arrows show how shape changes from normal to dwarf Whitefish.
Mentions: Broken-stick distribution indicated that only the two first PCs were informative. The first axis represents 50.6% of the variation and show variation among lakes (Figure 2). With the exception of Témiscouata Lake, dwarf Whitefish species also showed lower mean score on this axis than normal Whitefish (Figure 2). The second axis represents 35.7% of the variation and showed a clear distinction between all dwarf and normal Whitefish (Figure 2). Euclidian distance showed an increasing gradient in the following order: Webster (0.020; P-value = 0.032), Témiscouata (0.021; P-value < 0.001), Cliff (0.028; P-value < 0.001), East (0.032; P-value < 0.001), and Indian (0.038; P-value < 0.001). This gradient roughly correspond to the one observed with the juxtaposition of mean shape (Figure 3). In addition, all between-group PCA angles point in a similar direction (9−55°). Interestingly, the lower angle observed (Cliff: 9°) is linked to a ventral thinning in dwarf in comparison to normal Whitefish (Figure 3), the greater angle (Témiscouata: 55°) is linked to a dorsal thinning in dwarf (Figure 3) and the intermediary angles (Indian: 18°, Webster: 22°, and East: 25°) are linked to both ventral and dorsal thinning (Figure 3). Dwarf Whitefish also have bigger eyes and longer tails than the normal fish in all of the five lakes (Figure 3).

Bottom Line: Using a genetic map comprising 3438 restriction site-associated DNA sequencing single-nucleotide polymorphisms, we then identified quantitative trait loci underlying body shape traits in a backcross family reared in the laboratory.The single-locus approach provided limited evidence for genetic parallelism.These results provide evidence for both genetic parallelism and multiple genetic routes underlying parallel phenotypic evolution in fish shape among populations occupying similar ecological niches.

View Article: PubMed Central - PubMed

Affiliation: Institut de Biologie Intégrative et des Systèmes (IBIS), Département de Biologie, Université Laval, Pavillon Charles-Eugène-Marchand, Québec, G1V 0A6, Canada uni.mlaporte@gmail.com.

No MeSH data available.


Related in: MedlinePlus