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The impact of the absence of aliphatic glucosinolates on water transport under salt stress in Arabidopsis thaliana.

Martínez-Ballesta M, Moreno-Fernández DA, Castejón D, Ochando C, Morandini PA, Carvajal M - Front Plant Sci (2015)

Bottom Line: The double mutant myb28myb29, completely lacking aliphatic glucosinolates, was compared to wild type Col-0 (WT) and the single mutant myb28.A greater reduction in the hydraulic conductivity of myb28myb29 was observed under salt stress, when compared to the WT and myb28; this correlated with the abundance of both PIP1 and PIP2 aquaporin subfamilies.The results indicate that short-chain aliphatic glucosinolates may contribute to water saving under salt stress.

View Article: PubMed Central - PubMed

Affiliation: Plant Nutrition Department, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC) Murcia, Spain.

ABSTRACT
Members of the Brassicaceae are known for their contents of nutrients and health-promoting phytochemicals, including glucosinolates. Exposure to salinity increases the levels of several of these compounds, but their role in abiotic stress response is unclear. The effect of aliphatic glucosinolates on plant water balance and growth under salt stress, involving aquaporins, was investigated by means of Arabidopsis thaliana mutants impaired in aliphatic glucosinolate biosynthesis, which is controlled by two transcription factors: Myb28 and Myb29. The double mutant myb28myb29, completely lacking aliphatic glucosinolates, was compared to wild type Col-0 (WT) and the single mutant myb28. A greater reduction in the hydraulic conductivity of myb28myb29 was observed under salt stress, when compared to the WT and myb28; this correlated with the abundance of both PIP1 and PIP2 aquaporin subfamilies. Also, changes in root architecture in response to salinity were genotype dependent. Treatment with NaCl altered glucosinolates biosynthesis in a similar way in WT and the single mutant and differently in the double mutant. The results indicate that short-chain aliphatic glucosinolates may contribute to water saving under salt stress.

No MeSH data available.


Related in: MedlinePlus

Root hydraulic conductance, L0, (mg h−1 MPa−1 g−1 DW) (A), relative water content (RWC) (B) and whole plant transpiration (mg m2 s−1) (C) ofArabidopsis thalianaecotype Col-0 wild type (WT), single (myb28) and double (myb28myb29) knockout mutants under non-saline (0 mM NaCl) and saline (100 mM NaCl) conditions. Mean values ± standard errors are shown (n = 10). Mean values with different letters represent significant (P < 0.05) differences according to the Tukey test.
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Figure 1: Root hydraulic conductance, L0, (mg h−1 MPa−1 g−1 DW) (A), relative water content (RWC) (B) and whole plant transpiration (mg m2 s−1) (C) ofArabidopsis thalianaecotype Col-0 wild type (WT), single (myb28) and double (myb28myb29) knockout mutants under non-saline (0 mM NaCl) and saline (100 mM NaCl) conditions. Mean values ± standard errors are shown (n = 10). Mean values with different letters represent significant (P < 0.05) differences according to the Tukey test.

Mentions: The root hydraulic conductance (L0) was determined for all three genotypes by root pressurization (Figure 1A). In the absence of the salt treatment, the L0 of the myb28myb29 mutant was comparable to that of the WT and myb28 mutant. Salinity reduced L0 by 49.1 and 46.3% in the WT and single mutant, respectively, and by 63.0% in the double mutant.


The impact of the absence of aliphatic glucosinolates on water transport under salt stress in Arabidopsis thaliana.

Martínez-Ballesta M, Moreno-Fernández DA, Castejón D, Ochando C, Morandini PA, Carvajal M - Front Plant Sci (2015)

Root hydraulic conductance, L0, (mg h−1 MPa−1 g−1 DW) (A), relative water content (RWC) (B) and whole plant transpiration (mg m2 s−1) (C) ofArabidopsis thalianaecotype Col-0 wild type (WT), single (myb28) and double (myb28myb29) knockout mutants under non-saline (0 mM NaCl) and saline (100 mM NaCl) conditions. Mean values ± standard errors are shown (n = 10). Mean values with different letters represent significant (P < 0.05) differences according to the Tukey test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4502342&req=5

Figure 1: Root hydraulic conductance, L0, (mg h−1 MPa−1 g−1 DW) (A), relative water content (RWC) (B) and whole plant transpiration (mg m2 s−1) (C) ofArabidopsis thalianaecotype Col-0 wild type (WT), single (myb28) and double (myb28myb29) knockout mutants under non-saline (0 mM NaCl) and saline (100 mM NaCl) conditions. Mean values ± standard errors are shown (n = 10). Mean values with different letters represent significant (P < 0.05) differences according to the Tukey test.
Mentions: The root hydraulic conductance (L0) was determined for all three genotypes by root pressurization (Figure 1A). In the absence of the salt treatment, the L0 of the myb28myb29 mutant was comparable to that of the WT and myb28 mutant. Salinity reduced L0 by 49.1 and 46.3% in the WT and single mutant, respectively, and by 63.0% in the double mutant.

Bottom Line: The double mutant myb28myb29, completely lacking aliphatic glucosinolates, was compared to wild type Col-0 (WT) and the single mutant myb28.A greater reduction in the hydraulic conductivity of myb28myb29 was observed under salt stress, when compared to the WT and myb28; this correlated with the abundance of both PIP1 and PIP2 aquaporin subfamilies.The results indicate that short-chain aliphatic glucosinolates may contribute to water saving under salt stress.

View Article: PubMed Central - PubMed

Affiliation: Plant Nutrition Department, Centro de Edafología y Biología Aplicada del Segura (CEBAS-CSIC) Murcia, Spain.

ABSTRACT
Members of the Brassicaceae are known for their contents of nutrients and health-promoting phytochemicals, including glucosinolates. Exposure to salinity increases the levels of several of these compounds, but their role in abiotic stress response is unclear. The effect of aliphatic glucosinolates on plant water balance and growth under salt stress, involving aquaporins, was investigated by means of Arabidopsis thaliana mutants impaired in aliphatic glucosinolate biosynthesis, which is controlled by two transcription factors: Myb28 and Myb29. The double mutant myb28myb29, completely lacking aliphatic glucosinolates, was compared to wild type Col-0 (WT) and the single mutant myb28. A greater reduction in the hydraulic conductivity of myb28myb29 was observed under salt stress, when compared to the WT and myb28; this correlated with the abundance of both PIP1 and PIP2 aquaporin subfamilies. Also, changes in root architecture in response to salinity were genotype dependent. Treatment with NaCl altered glucosinolates biosynthesis in a similar way in WT and the single mutant and differently in the double mutant. The results indicate that short-chain aliphatic glucosinolates may contribute to water saving under salt stress.

No MeSH data available.


Related in: MedlinePlus