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Evaluation of Cellular Antioxidant and Antiproliferative Activities of Five Main Phyllanthus Emblica L. Cultivars in China.

Li Y, Sun HY, Yu XY, Liu D, Wan HX - Indian J Pharm Sci (2015 May-Jun)

Bottom Line: The results showed that Qingyougan, Binggan and Boligan (832±100, 774±52 and 704±28 μmol of quercetin equivalents/100 g) had higher cellular antioxidant activity than Tianyougan and Yougan (553±50 and 457±24 μmol of quercetin equivalents/100 g) in phosphate buffered saline wash protocol whereas, Boligan (3735±217 μmol of quercetin equivalents/100 g) had the highest cellular antioxidant activity and Tianyougan (2025±171 μmol of quercetin equivalents/100 g) had the lowest cellular antioxidant activity in no phosphate buffered saline wash protocol.The highest and lowest antiproliferative activities were observed in Binggan and Tianyougan (median effective dose: 6.95±0.11 and 14.03±0.10 mg/ml), respectively.The significant correlation was only observed between total flavonoids and cellular antioxidant activity from no phosphate buffered saline wash protocol (R(2) =0.908, P<0.05), and total flavonoids and antiproliferative activity (R(2) =0.887, P<0.05), suggesting the major contribution of flavonoids to the bioactivities of emblica.

View Article: PubMed Central - PubMed

Affiliation: Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen-518 055, China.

ABSTRACT
The cell-based antioxidant activity assay as more biological relevant assay was considered to be more accurate to predict antioxidant activity in vivo than chemical activity assays. In the present study, the five main Phyllanthus emblica L. cultivars in China were subjected for cellular antioxidant activity based on HepG2 cells as well as antiproliferative activity. Total phenolics, total flavonoids and oxygen radical absorbance capacity were also measured. The results showed that Qingyougan, Binggan and Boligan (832±100, 774±52 and 704±28 μmol of quercetin equivalents/100 g) had higher cellular antioxidant activity than Tianyougan and Yougan (553±50 and 457±24 μmol of quercetin equivalents/100 g) in phosphate buffered saline wash protocol whereas, Boligan (3735±217 μmol of quercetin equivalents/100 g) had the highest cellular antioxidant activity and Tianyougan (2025±171 μmol of quercetin equivalents/100 g) had the lowest cellular antioxidant activity in no phosphate buffered saline wash protocol. The highest and lowest antiproliferative activities were observed in Binggan and Tianyougan (median effective dose: 6.95±0.11 and 14.03±0.10 mg/ml), respectively. The significant correlation was only observed between total flavonoids and cellular antioxidant activity from no phosphate buffered saline wash protocol (R(2) =0.908, P<0.05), and total flavonoids and antiproliferative activity (R(2) =0.887, P<0.05), suggesting the major contribution of flavonoids to the bioactivities of emblica. Overall, the data obtained revealed that different Phyllanthus emblica L. cultivars had strong cellular antioxidant and antiproliferative activities, thus should be recommended to increase consumption for health.

No MeSH data available.


Total phenolic content of the five emblica cultivars.All data were shown as mean±SD from triplicate experiments. Bars with no letters in common are significantly different (P<0.05).
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Figure 1: Total phenolic content of the five emblica cultivars.All data were shown as mean±SD from triplicate experiments. Bars with no letters in common are significantly different (P<0.05).

Mentions: The total phenolic content of the five main Phyllanthus emblica L. cultivars in China was determined from their 70% acetone extracts by using the Folin-Ciocalteu method (fig. 1). Seventy percent acetone was used as extraction solvent in this study based on our preliminary experiments, which, showed that the emblica extract from 70% acetone had higher content of phenolics and higher level of antioxidant activity than the extract from other solvents. Singh et al.[31], also reported that the acetone extract exhibited better hydroxyl radical scavenging activity than methanol or chloroform extract for the emblica containing triphala. It could be found from fig. 1 that Yougan had the lower phenolic content (1.142±0.084 g of GAE/100 g) as being compared with other four emblica cultivars extracts (P<0.05), and there was no significant difference in the total phenolic contents between Qingyougan (1.518±0.037 g of GAE/100 g), Boligan (1.407±0.095 g of GAE/100 g), Tianyougan (1.382±0.013 g of GAE/100 g) and Binggan (1.362±0.037 g of GAE/100 g), averaging 1.417 g of GAE/100 g. There was a 75% difference in phenolic content between the highest and the lowest cultivars (P<0.05). It should be noted that the phenolic content determined in this study was lower than the previous reports[1832]. The main reason lied in that data expressed here were based on the 100 g fresh fruit rather than 100 g dried fruit.


Evaluation of Cellular Antioxidant and Antiproliferative Activities of Five Main Phyllanthus Emblica L. Cultivars in China.

Li Y, Sun HY, Yu XY, Liu D, Wan HX - Indian J Pharm Sci (2015 May-Jun)

Total phenolic content of the five emblica cultivars.All data were shown as mean±SD from triplicate experiments. Bars with no letters in common are significantly different (P<0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4502141&req=5

Figure 1: Total phenolic content of the five emblica cultivars.All data were shown as mean±SD from triplicate experiments. Bars with no letters in common are significantly different (P<0.05).
Mentions: The total phenolic content of the five main Phyllanthus emblica L. cultivars in China was determined from their 70% acetone extracts by using the Folin-Ciocalteu method (fig. 1). Seventy percent acetone was used as extraction solvent in this study based on our preliminary experiments, which, showed that the emblica extract from 70% acetone had higher content of phenolics and higher level of antioxidant activity than the extract from other solvents. Singh et al.[31], also reported that the acetone extract exhibited better hydroxyl radical scavenging activity than methanol or chloroform extract for the emblica containing triphala. It could be found from fig. 1 that Yougan had the lower phenolic content (1.142±0.084 g of GAE/100 g) as being compared with other four emblica cultivars extracts (P<0.05), and there was no significant difference in the total phenolic contents between Qingyougan (1.518±0.037 g of GAE/100 g), Boligan (1.407±0.095 g of GAE/100 g), Tianyougan (1.382±0.013 g of GAE/100 g) and Binggan (1.362±0.037 g of GAE/100 g), averaging 1.417 g of GAE/100 g. There was a 75% difference in phenolic content between the highest and the lowest cultivars (P<0.05). It should be noted that the phenolic content determined in this study was lower than the previous reports[1832]. The main reason lied in that data expressed here were based on the 100 g fresh fruit rather than 100 g dried fruit.

Bottom Line: The results showed that Qingyougan, Binggan and Boligan (832±100, 774±52 and 704±28 μmol of quercetin equivalents/100 g) had higher cellular antioxidant activity than Tianyougan and Yougan (553±50 and 457±24 μmol of quercetin equivalents/100 g) in phosphate buffered saline wash protocol whereas, Boligan (3735±217 μmol of quercetin equivalents/100 g) had the highest cellular antioxidant activity and Tianyougan (2025±171 μmol of quercetin equivalents/100 g) had the lowest cellular antioxidant activity in no phosphate buffered saline wash protocol.The highest and lowest antiproliferative activities were observed in Binggan and Tianyougan (median effective dose: 6.95±0.11 and 14.03±0.10 mg/ml), respectively.The significant correlation was only observed between total flavonoids and cellular antioxidant activity from no phosphate buffered saline wash protocol (R(2) =0.908, P<0.05), and total flavonoids and antiproliferative activity (R(2) =0.887, P<0.05), suggesting the major contribution of flavonoids to the bioactivities of emblica.

View Article: PubMed Central - PubMed

Affiliation: Shenzhen Key Laboratory of Fermentation, Purification and Analysis, Shenzhen Polytechnic, Shenzhen-518 055, China.

ABSTRACT
The cell-based antioxidant activity assay as more biological relevant assay was considered to be more accurate to predict antioxidant activity in vivo than chemical activity assays. In the present study, the five main Phyllanthus emblica L. cultivars in China were subjected for cellular antioxidant activity based on HepG2 cells as well as antiproliferative activity. Total phenolics, total flavonoids and oxygen radical absorbance capacity were also measured. The results showed that Qingyougan, Binggan and Boligan (832±100, 774±52 and 704±28 μmol of quercetin equivalents/100 g) had higher cellular antioxidant activity than Tianyougan and Yougan (553±50 and 457±24 μmol of quercetin equivalents/100 g) in phosphate buffered saline wash protocol whereas, Boligan (3735±217 μmol of quercetin equivalents/100 g) had the highest cellular antioxidant activity and Tianyougan (2025±171 μmol of quercetin equivalents/100 g) had the lowest cellular antioxidant activity in no phosphate buffered saline wash protocol. The highest and lowest antiproliferative activities were observed in Binggan and Tianyougan (median effective dose: 6.95±0.11 and 14.03±0.10 mg/ml), respectively. The significant correlation was only observed between total flavonoids and cellular antioxidant activity from no phosphate buffered saline wash protocol (R(2) =0.908, P<0.05), and total flavonoids and antiproliferative activity (R(2) =0.887, P<0.05), suggesting the major contribution of flavonoids to the bioactivities of emblica. Overall, the data obtained revealed that different Phyllanthus emblica L. cultivars had strong cellular antioxidant and antiproliferative activities, thus should be recommended to increase consumption for health.

No MeSH data available.