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Effects of extracts of Salvadora persica on proliferation and viability of human dental pulp stem cells.

Tabatabaei FS, Moezizadeh M, Javand F - J Conserv Dent (2015 Jul-Aug)

Bottom Line: Other concentrations had no significant effects on cells (P > 0.05).The highest concentrations of both water and ethanolic extracts of SP had cytotoxic effects on hDPSCs.Water extract of SP has favorable effects on cell proliferation at specific concentrations in a time-dependent manner.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Biomaterials, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Objectives: Efficacy of an ideal antimicrobial agent depends on its ability to eliminate microorganisms while causing minimal toxicity to host cells. The purpose of this study was to assess the effect of ethanolic and water extracts of Salvadora persica (SP) on proliferation and viability of human dental pulp stem cells (hDPSCs).

Materials and methods: In this in-vitro study, the effects of seven concentrations of ethanolic and water extracts of SP (ranging from 5.75 mg/ml to 0.08 mg/ml) on hDPSCs were evaluated using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay. The results were analyzed using one-way ANOVA and Tukey's post-hoc test. P < 0.05 was considered statistically significant.

Results: Water extract of SP only had cytotoxic effect at 5.75 mg/ml concentration; and caused significant cell proliferation at 1.43-0.08 mg/ml concentrations at 24 h (P < 0.05). At 48 h, only 0.17 and 0.08 mg/ml concentrations caused significant cell proliferation (P < 0.05). Ethanolic extract of SP at 5.75-1.43 mg/ml concentrations showed severe cytotoxic effects at 24 and 48 h. Other concentrations had no significant effects on cells (P > 0.05).

Conclusion: The highest concentrations of both water and ethanolic extracts of SP had cytotoxic effects on hDPSCs. Water extract of SP has favorable effects on cell proliferation at specific concentrations in a time-dependent manner.

No MeSH data available.


Related in: MedlinePlus

Percentage of viability of human dental pulp stem cells after exposure to various concentrations of ethanolic and water extracts of Salvadora persica at 48 h. *Indicates a significant difference (P < 0.05)
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Figure 2: Percentage of viability of human dental pulp stem cells after exposure to various concentrations of ethanolic and water extracts of Salvadora persica at 48 h. *Indicates a significant difference (P < 0.05)

Mentions: Figures 1 and 2 illustrate the influence of the different elutes of two extracts on the growth of the hDPSCs.


Effects of extracts of Salvadora persica on proliferation and viability of human dental pulp stem cells.

Tabatabaei FS, Moezizadeh M, Javand F - J Conserv Dent (2015 Jul-Aug)

Percentage of viability of human dental pulp stem cells after exposure to various concentrations of ethanolic and water extracts of Salvadora persica at 48 h. *Indicates a significant difference (P < 0.05)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4502129&req=5

Figure 2: Percentage of viability of human dental pulp stem cells after exposure to various concentrations of ethanolic and water extracts of Salvadora persica at 48 h. *Indicates a significant difference (P < 0.05)
Mentions: Figures 1 and 2 illustrate the influence of the different elutes of two extracts on the growth of the hDPSCs.

Bottom Line: Other concentrations had no significant effects on cells (P > 0.05).The highest concentrations of both water and ethanolic extracts of SP had cytotoxic effects on hDPSCs.Water extract of SP has favorable effects on cell proliferation at specific concentrations in a time-dependent manner.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Biomaterials, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Objectives: Efficacy of an ideal antimicrobial agent depends on its ability to eliminate microorganisms while causing minimal toxicity to host cells. The purpose of this study was to assess the effect of ethanolic and water extracts of Salvadora persica (SP) on proliferation and viability of human dental pulp stem cells (hDPSCs).

Materials and methods: In this in-vitro study, the effects of seven concentrations of ethanolic and water extracts of SP (ranging from 5.75 mg/ml to 0.08 mg/ml) on hDPSCs were evaluated using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay. The results were analyzed using one-way ANOVA and Tukey's post-hoc test. P < 0.05 was considered statistically significant.

Results: Water extract of SP only had cytotoxic effect at 5.75 mg/ml concentration; and caused significant cell proliferation at 1.43-0.08 mg/ml concentrations at 24 h (P < 0.05). At 48 h, only 0.17 and 0.08 mg/ml concentrations caused significant cell proliferation (P < 0.05). Ethanolic extract of SP at 5.75-1.43 mg/ml concentrations showed severe cytotoxic effects at 24 and 48 h. Other concentrations had no significant effects on cells (P > 0.05).

Conclusion: The highest concentrations of both water and ethanolic extracts of SP had cytotoxic effects on hDPSCs. Water extract of SP has favorable effects on cell proliferation at specific concentrations in a time-dependent manner.

No MeSH data available.


Related in: MedlinePlus