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OsRDR6 plays role in host defense against double-stranded RNA virus, Rice Dwarf Phytoreovirus.

Hong W, Qian D, Sun R, Jiang L, Wang Y, Wei C, Zhang Z, Li Y - Sci Rep (2015)

Bottom Line: We found previously that rice RDR6 (OsRDR6) functioned in the defense against Rice stripe virus (RSV), and Rice Dwarf Phytoreovirus (RDV) infection resulted in down-regulation of expression of RDR6.Our result showed that down-regulation of OsRDR6 through the antisense (OsRDR6AS) strategy increased rice susceptibility to RDV infection while over-expression of OsRDR6 had no effect on RDV infection.The accumulation of RDV vsiRNAs was reduced in the OsRDR6AS plants.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.

ABSTRACT
RNAi is a major antiviral defense response in plant and animal model systems. RNA-dependent RNA polymerase 6 (RDR6) is an essential component of RNAi, which plays an important role in the resistance against viruses in the model plants. We found previously that rice RDR6 (OsRDR6) functioned in the defense against Rice stripe virus (RSV), and Rice Dwarf Phytoreovirus (RDV) infection resulted in down-regulation of expression of RDR6. Here we report our new findings on the function of OsRDR6 against RDV. Our result showed that down-regulation of OsRDR6 through the antisense (OsRDR6AS) strategy increased rice susceptibility to RDV infection while over-expression of OsRDR6 had no effect on RDV infection. The accumulation of RDV vsiRNAs was reduced in the OsRDR6AS plants. In the OsRDR6 over-expressed plants, the levels of OsRDR6 RNA transcript and protein were much higher than that in the control plants. Interestingly, the accumulation level of OsRDR6 protein became undetectable after RDV infection. This finding indicated that the translation and/or stability of OsRDR6 protein were negatively impacted upon RDV infection. This new finding provides a new light on the function of RDR6 in plant defense response and the cross-talking between factors encoded by host plant and double-stranded RNA viruses.

No MeSH data available.


Related in: MedlinePlus

Over-expression of OsRDR6 and AtRDR6 in rdr6 mutant A. thaliana restored the accumulation of TAS siRNAs in plants.Four TASs [TAS1 siRNA255, TAS2 siRNA1511, TAS3 5'D7 (+) and TAS3 5'D8 (+)] were analyzed using Northern blot assay. Accumulation of miR173, miR390 and miR391 in the OsRDR6/rdr6–11, AtRDR6/rdr6–11 or OsRDR2/rdr6–11 transgenic Arabidopsis plants were also analyzed in the assay. tRNA isolated from each sample was served as the loading control.
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f5: Over-expression of OsRDR6 and AtRDR6 in rdr6 mutant A. thaliana restored the accumulation of TAS siRNAs in plants.Four TASs [TAS1 siRNA255, TAS2 siRNA1511, TAS3 5'D7 (+) and TAS3 5'D8 (+)] were analyzed using Northern blot assay. Accumulation of miR173, miR390 and miR391 in the OsRDR6/rdr6–11, AtRDR6/rdr6–11 or OsRDR2/rdr6–11 transgenic Arabidopsis plants were also analyzed in the assay. tRNA isolated from each sample was served as the loading control.

Mentions: To verify the biological function of OsRDR6 protein in the OE lines, we conducted a complementation assay by over-expressing the full length coding sequence of OsRDR6 and AtRDR6 in the rdr6–11 mutant Arabidopsis2324 to produce OsRDR6/rdr6–11 and AtRDR6/rdr6–11 transgenic lines, respectively. OsRDR2/rdr6–11 line was produced to serve as a control. Analysis of these lines showed that OsRDR6 and AtRDR6 indeed restored the accumulation of TAS siRNAs52 in the over-expression Arabidopsis transgenic plants whereas the transgenic plants over-expression OsRDR2 did not (Fig. 5). Therefore the inability of OsRDR6 protein in the OE rice line to suppress RDV infection was not due to the authenticity of gene sequence transformed into the rice plants.


OsRDR6 plays role in host defense against double-stranded RNA virus, Rice Dwarf Phytoreovirus.

Hong W, Qian D, Sun R, Jiang L, Wang Y, Wei C, Zhang Z, Li Y - Sci Rep (2015)

Over-expression of OsRDR6 and AtRDR6 in rdr6 mutant A. thaliana restored the accumulation of TAS siRNAs in plants.Four TASs [TAS1 siRNA255, TAS2 siRNA1511, TAS3 5'D7 (+) and TAS3 5'D8 (+)] were analyzed using Northern blot assay. Accumulation of miR173, miR390 and miR391 in the OsRDR6/rdr6–11, AtRDR6/rdr6–11 or OsRDR2/rdr6–11 transgenic Arabidopsis plants were also analyzed in the assay. tRNA isolated from each sample was served as the loading control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499934&req=5

f5: Over-expression of OsRDR6 and AtRDR6 in rdr6 mutant A. thaliana restored the accumulation of TAS siRNAs in plants.Four TASs [TAS1 siRNA255, TAS2 siRNA1511, TAS3 5'D7 (+) and TAS3 5'D8 (+)] were analyzed using Northern blot assay. Accumulation of miR173, miR390 and miR391 in the OsRDR6/rdr6–11, AtRDR6/rdr6–11 or OsRDR2/rdr6–11 transgenic Arabidopsis plants were also analyzed in the assay. tRNA isolated from each sample was served as the loading control.
Mentions: To verify the biological function of OsRDR6 protein in the OE lines, we conducted a complementation assay by over-expressing the full length coding sequence of OsRDR6 and AtRDR6 in the rdr6–11 mutant Arabidopsis2324 to produce OsRDR6/rdr6–11 and AtRDR6/rdr6–11 transgenic lines, respectively. OsRDR2/rdr6–11 line was produced to serve as a control. Analysis of these lines showed that OsRDR6 and AtRDR6 indeed restored the accumulation of TAS siRNAs52 in the over-expression Arabidopsis transgenic plants whereas the transgenic plants over-expression OsRDR2 did not (Fig. 5). Therefore the inability of OsRDR6 protein in the OE rice line to suppress RDV infection was not due to the authenticity of gene sequence transformed into the rice plants.

Bottom Line: We found previously that rice RDR6 (OsRDR6) functioned in the defense against Rice stripe virus (RSV), and Rice Dwarf Phytoreovirus (RDV) infection resulted in down-regulation of expression of RDR6.Our result showed that down-regulation of OsRDR6 through the antisense (OsRDR6AS) strategy increased rice susceptibility to RDV infection while over-expression of OsRDR6 had no effect on RDV infection.The accumulation of RDV vsiRNAs was reduced in the OsRDR6AS plants.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.

ABSTRACT
RNAi is a major antiviral defense response in plant and animal model systems. RNA-dependent RNA polymerase 6 (RDR6) is an essential component of RNAi, which plays an important role in the resistance against viruses in the model plants. We found previously that rice RDR6 (OsRDR6) functioned in the defense against Rice stripe virus (RSV), and Rice Dwarf Phytoreovirus (RDV) infection resulted in down-regulation of expression of RDR6. Here we report our new findings on the function of OsRDR6 against RDV. Our result showed that down-regulation of OsRDR6 through the antisense (OsRDR6AS) strategy increased rice susceptibility to RDV infection while over-expression of OsRDR6 had no effect on RDV infection. The accumulation of RDV vsiRNAs was reduced in the OsRDR6AS plants. In the OsRDR6 over-expressed plants, the levels of OsRDR6 RNA transcript and protein were much higher than that in the control plants. Interestingly, the accumulation level of OsRDR6 protein became undetectable after RDV infection. This finding indicated that the translation and/or stability of OsRDR6 protein were negatively impacted upon RDV infection. This new finding provides a new light on the function of RDR6 in plant defense response and the cross-talking between factors encoded by host plant and double-stranded RNA viruses.

No MeSH data available.


Related in: MedlinePlus