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The effect of mepivacaine on swine lingual, pulmonary and coronary arteries.

Satoh K, Chikuda M, Ohashi A, Kumagai M, Sato M, Joh S - BMC Anesthesiol (2015)

Bottom Line: The present study aims to investigate the direct effects of mepivacaine on swine lingual, pulmonary and coronary arterial endothelium-denuded rings.Mepivacaine at 0.4 to 40 μM did not significantly alter 40 mM KCl-induced contraction in the lingual, pulmonary and coronary arterial rings.In contrast, mepivacaine at 4 mM produced attenuated vasoconstriction in the lingual, pulmonary and coronary arterial compared with isotonic 40 mM KCl.

View Article: PubMed Central - PubMed

Affiliation: Division of Dental Anesthesiology, Department of Reconstructive Oral and Maxillofacial Surgery, School of Dentistry, Iwate Medical University, 1-3-27 Chuo-dori, Morioka, Iwate, 020-8505, Japan. satoken@iwate-med.ac.jp.

ABSTRACT

Background: Although mepivacaine has a known biphasic action on the aortic and coronary artery in several animal species, its effects on the lingual and pulmonary artery are not well understood and it is not yet known whether mepivacaine produces vasoconstriction in these vessels. The present study aims to investigate the direct effects of mepivacaine on swine lingual, pulmonary and coronary arterial endothelium-denuded rings.

Methods: Artery rings were perfused with isotonic 40 mM KCl until a stable constricted plateau was reached. The rings were then perfused with isotonic 40 mM KCl plus a particular concentration of mepivacaine (0.4 μM, 4.0 μM, 40 μM, 0.4 mM and 4.0 mM). The isometric tension strengths in each experiment were normalized to the strength of the isometric tension immediately before mepivacaine perfusion and expressed as a percentage.

Results: Mepivacaine at 0.4 to 40 μM did not significantly alter 40 mM KCl-induced contraction in the lingual, pulmonary and coronary arterial rings. In contrast, mepivacaine at 4 mM produced attenuated vasoconstriction in the lingual, pulmonary and coronary arterial compared with isotonic 40 mM KCl.

Conclusions: Mepivacaine produced vasoconstriction at lower concentrations, followed by attenuated vasoconstriction at higher concentrations on swine lingual, pulmonary and coronary arterial endothelium-denuded rings. Mepivacaine (4 μM) appeared to increase isotonic 40 mM KCl-induced contraction, followed by attenuated vasoconstriction at 4 mM. Dentists using 3 % mepivacaine should take into consideration that the risk of complications may be increased if more than six mepivacaine cartridges are used in dental treatment or minor surgery, or if over 15 ml of mepivacaine is administered to a patient with cardiovascular complications during general anesthesia for oral maxillofacial surgery.

No MeSH data available.


Related in: MedlinePlus

A typical trace showing the effect of mepivacaine on pulmonary artery ring contraction induced with 40 mM KCl. a Representative recording of the mepivacaine-induced contraction. b Enlarged view of a square portion of (a)
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Fig3: A typical trace showing the effect of mepivacaine on pulmonary artery ring contraction induced with 40 mM KCl. a Representative recording of the mepivacaine-induced contraction. b Enlarged view of a square portion of (a)

Mentions: There was no response induced by mepivacaine in lingual, pulmonary and coronary arterial rings under resting conditions in HBSS (Fig. 1). Mepivacaine resulted in dilation of the swine lingual, pulmonary and coronary arterial rings that were maintained in a stable, constricted state; typical traces of the changes in isometric tension in response to increasing mepivacaine concentrations (0.4 μM to 4 mM) are shown in Figs 2, 3 and 4. Dose response curves at these concentrations are shown in Fig. 5. Mepivacaine at 0.4 to 40 μM did not significantly alter 40 mM KCl-induced contraction in the lingual, pulmonary and coronary arterial rings (Table 1). In contrast, the highest concentration of mepivacaine (4 mM) produced attenuated vasoconstriction. There were significant differences between the effects of the control solution (40 mM KCl) and the effect of 4 mM mepivacaine on the lingual artery, 400 μM and 4 mM mepivacaine on the pulmonary artery, and 4 mM mepivacaine on the coronary artery.Fig. 1


The effect of mepivacaine on swine lingual, pulmonary and coronary arteries.

Satoh K, Chikuda M, Ohashi A, Kumagai M, Sato M, Joh S - BMC Anesthesiol (2015)

A typical trace showing the effect of mepivacaine on pulmonary artery ring contraction induced with 40 mM KCl. a Representative recording of the mepivacaine-induced contraction. b Enlarged view of a square portion of (a)
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4499916&req=5

Fig3: A typical trace showing the effect of mepivacaine on pulmonary artery ring contraction induced with 40 mM KCl. a Representative recording of the mepivacaine-induced contraction. b Enlarged view of a square portion of (a)
Mentions: There was no response induced by mepivacaine in lingual, pulmonary and coronary arterial rings under resting conditions in HBSS (Fig. 1). Mepivacaine resulted in dilation of the swine lingual, pulmonary and coronary arterial rings that were maintained in a stable, constricted state; typical traces of the changes in isometric tension in response to increasing mepivacaine concentrations (0.4 μM to 4 mM) are shown in Figs 2, 3 and 4. Dose response curves at these concentrations are shown in Fig. 5. Mepivacaine at 0.4 to 40 μM did not significantly alter 40 mM KCl-induced contraction in the lingual, pulmonary and coronary arterial rings (Table 1). In contrast, the highest concentration of mepivacaine (4 mM) produced attenuated vasoconstriction. There were significant differences between the effects of the control solution (40 mM KCl) and the effect of 4 mM mepivacaine on the lingual artery, 400 μM and 4 mM mepivacaine on the pulmonary artery, and 4 mM mepivacaine on the coronary artery.Fig. 1

Bottom Line: The present study aims to investigate the direct effects of mepivacaine on swine lingual, pulmonary and coronary arterial endothelium-denuded rings.Mepivacaine at 0.4 to 40 μM did not significantly alter 40 mM KCl-induced contraction in the lingual, pulmonary and coronary arterial rings.In contrast, mepivacaine at 4 mM produced attenuated vasoconstriction in the lingual, pulmonary and coronary arterial compared with isotonic 40 mM KCl.

View Article: PubMed Central - PubMed

Affiliation: Division of Dental Anesthesiology, Department of Reconstructive Oral and Maxillofacial Surgery, School of Dentistry, Iwate Medical University, 1-3-27 Chuo-dori, Morioka, Iwate, 020-8505, Japan. satoken@iwate-med.ac.jp.

ABSTRACT

Background: Although mepivacaine has a known biphasic action on the aortic and coronary artery in several animal species, its effects on the lingual and pulmonary artery are not well understood and it is not yet known whether mepivacaine produces vasoconstriction in these vessels. The present study aims to investigate the direct effects of mepivacaine on swine lingual, pulmonary and coronary arterial endothelium-denuded rings.

Methods: Artery rings were perfused with isotonic 40 mM KCl until a stable constricted plateau was reached. The rings were then perfused with isotonic 40 mM KCl plus a particular concentration of mepivacaine (0.4 μM, 4.0 μM, 40 μM, 0.4 mM and 4.0 mM). The isometric tension strengths in each experiment were normalized to the strength of the isometric tension immediately before mepivacaine perfusion and expressed as a percentage.

Results: Mepivacaine at 0.4 to 40 μM did not significantly alter 40 mM KCl-induced contraction in the lingual, pulmonary and coronary arterial rings. In contrast, mepivacaine at 4 mM produced attenuated vasoconstriction in the lingual, pulmonary and coronary arterial compared with isotonic 40 mM KCl.

Conclusions: Mepivacaine produced vasoconstriction at lower concentrations, followed by attenuated vasoconstriction at higher concentrations on swine lingual, pulmonary and coronary arterial endothelium-denuded rings. Mepivacaine (4 μM) appeared to increase isotonic 40 mM KCl-induced contraction, followed by attenuated vasoconstriction at 4 mM. Dentists using 3 % mepivacaine should take into consideration that the risk of complications may be increased if more than six mepivacaine cartridges are used in dental treatment or minor surgery, or if over 15 ml of mepivacaine is administered to a patient with cardiovascular complications during general anesthesia for oral maxillofacial surgery.

No MeSH data available.


Related in: MedlinePlus