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Acquired transmissibility of sheep-passaged L-type bovine spongiform encephalopathy prion to wild-type mice.

Okada H, Masujin K, Miyazawa K, Yokoyama T - Vet. Res. (2015)

Bottom Line: Disease-associated prion protein (PrP(Sc)) was detected in the brain and/or lymphoid tissues during the lifespan of mice that were asymptomatic subclinical carriers, indicating that wild-type mice were susceptible to sheep-passaged L-BSE.The morphological characteristics of the PrP(Sc) of sheep-passaged L-BSE included florid plaques that were distributed mainly in the cerebral cortex and hippocampus of subsequent passaged mice.The PrP(Sc) glycoform profiles of wild-type mice infected with sheep-passaged L-BSE were similar to those of the original isolate.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan. okadahi@affrc.go.jp.

ABSTRACT
L-type bovine spongiform encephalopathy (L-BSE) is an atypical form of BSE that is transmissible to cattle and several lines of prion protein (PrP) transgenic mice, but not to wild-type mice. In this study, we examined the transmissibility of sheep-passaged L-BSE prions to wild-type mice. Disease-associated prion protein (PrP(Sc)) was detected in the brain and/or lymphoid tissues during the lifespan of mice that were asymptomatic subclinical carriers, indicating that wild-type mice were susceptible to sheep-passaged L-BSE. The morphological characteristics of the PrP(Sc) of sheep-passaged L-BSE included florid plaques that were distributed mainly in the cerebral cortex and hippocampus of subsequent passaged mice. The PrP(Sc) glycoform profiles of wild-type mice infected with sheep-passaged L-BSE were similar to those of the original isolate. The data indicate that sheep-passaged L-BSE has an altered host range and acquired transmissibility to wild-type mice.

No MeSH data available.


Related in: MedlinePlus

Western blot analysis of proteinase-K resistant L-BSE PrPSc in TgBoPrP mice before and after passage in sheep. The brain samples of TgBoPrP mice inoculated intracerebrally with either L-BSE/cattle or L-BSE/sheep were analyzed. PrPSc in the twice-passaged mice was detected with mAb T2. The numbers indicate three different mice.
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Fig4: Western blot analysis of proteinase-K resistant L-BSE PrPSc in TgBoPrP mice before and after passage in sheep. The brain samples of TgBoPrP mice inoculated intracerebrally with either L-BSE/cattle or L-BSE/sheep were analyzed. PrPSc in the twice-passaged mice was detected with mAb T2. The numbers indicate three different mice.

Mentions: Transmission of L-BSE/cattle to TgBoPrP mice resulted in a mean incubation period of 195 ± 6 (n = 18) and 152 ± 2 days (n = 24) at primary and secondary passage, respectively. In contrast, the mean incubation period of L-BSE/sheep to TgBoPrP mice was 249 ± 28 (n = 5) and 269 ± 17 days (n = 13) at primary and secondary passage, respectively, which indicated a significantly longer incubation period compared to L-BSE/cattle, the original isolate (Table 1). However, pathological changes including lesion profiles and distribution patterns or types of PrPSc, and molecular features of PK-resistant PrPSc in the brains of TgBoPrP mice inoculated with L-BSE/sheep were identical to those in mice inoculated with L-BSE/cattle (Figure 4). In addition, PK-resistant PrPSc in TgBoPrP mice inoculated with either L-BSE/sheep or L-BSE/cattle showed similar biochemical profiles to L-BSE/cattle, the original isolate.Figure 4


Acquired transmissibility of sheep-passaged L-type bovine spongiform encephalopathy prion to wild-type mice.

Okada H, Masujin K, Miyazawa K, Yokoyama T - Vet. Res. (2015)

Western blot analysis of proteinase-K resistant L-BSE PrPSc in TgBoPrP mice before and after passage in sheep. The brain samples of TgBoPrP mice inoculated intracerebrally with either L-BSE/cattle or L-BSE/sheep were analyzed. PrPSc in the twice-passaged mice was detected with mAb T2. The numbers indicate three different mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4499898&req=5

Fig4: Western blot analysis of proteinase-K resistant L-BSE PrPSc in TgBoPrP mice before and after passage in sheep. The brain samples of TgBoPrP mice inoculated intracerebrally with either L-BSE/cattle or L-BSE/sheep were analyzed. PrPSc in the twice-passaged mice was detected with mAb T2. The numbers indicate three different mice.
Mentions: Transmission of L-BSE/cattle to TgBoPrP mice resulted in a mean incubation period of 195 ± 6 (n = 18) and 152 ± 2 days (n = 24) at primary and secondary passage, respectively. In contrast, the mean incubation period of L-BSE/sheep to TgBoPrP mice was 249 ± 28 (n = 5) and 269 ± 17 days (n = 13) at primary and secondary passage, respectively, which indicated a significantly longer incubation period compared to L-BSE/cattle, the original isolate (Table 1). However, pathological changes including lesion profiles and distribution patterns or types of PrPSc, and molecular features of PK-resistant PrPSc in the brains of TgBoPrP mice inoculated with L-BSE/sheep were identical to those in mice inoculated with L-BSE/cattle (Figure 4). In addition, PK-resistant PrPSc in TgBoPrP mice inoculated with either L-BSE/sheep or L-BSE/cattle showed similar biochemical profiles to L-BSE/cattle, the original isolate.Figure 4

Bottom Line: Disease-associated prion protein (PrP(Sc)) was detected in the brain and/or lymphoid tissues during the lifespan of mice that were asymptomatic subclinical carriers, indicating that wild-type mice were susceptible to sheep-passaged L-BSE.The morphological characteristics of the PrP(Sc) of sheep-passaged L-BSE included florid plaques that were distributed mainly in the cerebral cortex and hippocampus of subsequent passaged mice.The PrP(Sc) glycoform profiles of wild-type mice infected with sheep-passaged L-BSE were similar to those of the original isolate.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan. okadahi@affrc.go.jp.

ABSTRACT
L-type bovine spongiform encephalopathy (L-BSE) is an atypical form of BSE that is transmissible to cattle and several lines of prion protein (PrP) transgenic mice, but not to wild-type mice. In this study, we examined the transmissibility of sheep-passaged L-BSE prions to wild-type mice. Disease-associated prion protein (PrP(Sc)) was detected in the brain and/or lymphoid tissues during the lifespan of mice that were asymptomatic subclinical carriers, indicating that wild-type mice were susceptible to sheep-passaged L-BSE. The morphological characteristics of the PrP(Sc) of sheep-passaged L-BSE included florid plaques that were distributed mainly in the cerebral cortex and hippocampus of subsequent passaged mice. The PrP(Sc) glycoform profiles of wild-type mice infected with sheep-passaged L-BSE were similar to those of the original isolate. The data indicate that sheep-passaged L-BSE has an altered host range and acquired transmissibility to wild-type mice.

No MeSH data available.


Related in: MedlinePlus