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Acquired transmissibility of sheep-passaged L-type bovine spongiform encephalopathy prion to wild-type mice.

Okada H, Masujin K, Miyazawa K, Yokoyama T - Vet. Res. (2015)

Bottom Line: Disease-associated prion protein (PrP(Sc)) was detected in the brain and/or lymphoid tissues during the lifespan of mice that were asymptomatic subclinical carriers, indicating that wild-type mice were susceptible to sheep-passaged L-BSE.The morphological characteristics of the PrP(Sc) of sheep-passaged L-BSE included florid plaques that were distributed mainly in the cerebral cortex and hippocampus of subsequent passaged mice.The PrP(Sc) glycoform profiles of wild-type mice infected with sheep-passaged L-BSE were similar to those of the original isolate.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan. okadahi@affrc.go.jp.

ABSTRACT
L-type bovine spongiform encephalopathy (L-BSE) is an atypical form of BSE that is transmissible to cattle and several lines of prion protein (PrP) transgenic mice, but not to wild-type mice. In this study, we examined the transmissibility of sheep-passaged L-BSE prions to wild-type mice. Disease-associated prion protein (PrP(Sc)) was detected in the brain and/or lymphoid tissues during the lifespan of mice that were asymptomatic subclinical carriers, indicating that wild-type mice were susceptible to sheep-passaged L-BSE. The morphological characteristics of the PrP(Sc) of sheep-passaged L-BSE included florid plaques that were distributed mainly in the cerebral cortex and hippocampus of subsequent passaged mice. The PrP(Sc) glycoform profiles of wild-type mice infected with sheep-passaged L-BSE were similar to those of the original isolate. The data indicate that sheep-passaged L-BSE has an altered host range and acquired transmissibility to wild-type mice.

No MeSH data available.


Related in: MedlinePlus

Immunolabeled PrPSc accumulation in the brain and lymph node of ICR mice infected with sheep-passaged L-BSE, at first passage. PrPSc accumulated in the brain of the medial preoptic nucleus (A), habenular nucleus (B), and midbrain tegmentum (C), and in the follicular dendritic cells within the secondary follicle of renal lymph node (D) of a mouse killed at 710 days post-inoculation. Immunohistochemical labeling with mAb SAF84 and hematoxylin counterstain. Scale bar = 25 μm.
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Fig1: Immunolabeled PrPSc accumulation in the brain and lymph node of ICR mice infected with sheep-passaged L-BSE, at first passage. PrPSc accumulated in the brain of the medial preoptic nucleus (A), habenular nucleus (B), and midbrain tegmentum (C), and in the follicular dendritic cells within the secondary follicle of renal lymph node (D) of a mouse killed at 710 days post-inoculation. Immunohistochemical labeling with mAb SAF84 and hematoxylin counterstain. Scale bar = 25 μm.

Mentions: The first-passaged mice with L-BSE/sheep showed no clinical signs of the disease and were sacrificed at the end of their lives, or at an earlier stage of deterioration in accordance with welfare concerns relating to animal experiments, during the period between 172 and 1012 days post-inoculation (dpi) (Table 1). A PrPSc signal was detected by WB, IHC, or both techniques in the brain from 1 case at 710 dpi, and in lymphoid tissues (including spleen, lymph nodes, tonsils, and Peyer’s patches) from 9 of 15 mice after 200 dpi after the first passage. Positive IHC results in a mouse were typically composed of sparse granular deposits in some areas of the brain such as the vestibular nucleus or dorsal motor nucleus of the vagal nerve, midbrain tegmentum, hypothalamus, medial preoptic nucleus, and habenular nucleus (Figure 1). In addition, intense PrPSc immunolabeling in the follicles of lymphoid tissues was confined to follicular dendritic cells (Figure 1D). The mAbs 2G11 and SAF84 produced positive results in the sections, but mAb 12F10 was negative. However, transmission of the original isolate of L-BSE/cattle into wild-type mice was inefficient, and resulted in no clinical signs of the disease at the end of the lifespan, and an absence of positive signals in the brains and lymphoid tissues by WB and IHC analyses (Table 1).Table 1


Acquired transmissibility of sheep-passaged L-type bovine spongiform encephalopathy prion to wild-type mice.

Okada H, Masujin K, Miyazawa K, Yokoyama T - Vet. Res. (2015)

Immunolabeled PrPSc accumulation in the brain and lymph node of ICR mice infected with sheep-passaged L-BSE, at first passage. PrPSc accumulated in the brain of the medial preoptic nucleus (A), habenular nucleus (B), and midbrain tegmentum (C), and in the follicular dendritic cells within the secondary follicle of renal lymph node (D) of a mouse killed at 710 days post-inoculation. Immunohistochemical labeling with mAb SAF84 and hematoxylin counterstain. Scale bar = 25 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4499898&req=5

Fig1: Immunolabeled PrPSc accumulation in the brain and lymph node of ICR mice infected with sheep-passaged L-BSE, at first passage. PrPSc accumulated in the brain of the medial preoptic nucleus (A), habenular nucleus (B), and midbrain tegmentum (C), and in the follicular dendritic cells within the secondary follicle of renal lymph node (D) of a mouse killed at 710 days post-inoculation. Immunohistochemical labeling with mAb SAF84 and hematoxylin counterstain. Scale bar = 25 μm.
Mentions: The first-passaged mice with L-BSE/sheep showed no clinical signs of the disease and were sacrificed at the end of their lives, or at an earlier stage of deterioration in accordance with welfare concerns relating to animal experiments, during the period between 172 and 1012 days post-inoculation (dpi) (Table 1). A PrPSc signal was detected by WB, IHC, or both techniques in the brain from 1 case at 710 dpi, and in lymphoid tissues (including spleen, lymph nodes, tonsils, and Peyer’s patches) from 9 of 15 mice after 200 dpi after the first passage. Positive IHC results in a mouse were typically composed of sparse granular deposits in some areas of the brain such as the vestibular nucleus or dorsal motor nucleus of the vagal nerve, midbrain tegmentum, hypothalamus, medial preoptic nucleus, and habenular nucleus (Figure 1). In addition, intense PrPSc immunolabeling in the follicles of lymphoid tissues was confined to follicular dendritic cells (Figure 1D). The mAbs 2G11 and SAF84 produced positive results in the sections, but mAb 12F10 was negative. However, transmission of the original isolate of L-BSE/cattle into wild-type mice was inefficient, and resulted in no clinical signs of the disease at the end of the lifespan, and an absence of positive signals in the brains and lymphoid tissues by WB and IHC analyses (Table 1).Table 1

Bottom Line: Disease-associated prion protein (PrP(Sc)) was detected in the brain and/or lymphoid tissues during the lifespan of mice that were asymptomatic subclinical carriers, indicating that wild-type mice were susceptible to sheep-passaged L-BSE.The morphological characteristics of the PrP(Sc) of sheep-passaged L-BSE included florid plaques that were distributed mainly in the cerebral cortex and hippocampus of subsequent passaged mice.The PrP(Sc) glycoform profiles of wild-type mice infected with sheep-passaged L-BSE were similar to those of the original isolate.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan. okadahi@affrc.go.jp.

ABSTRACT
L-type bovine spongiform encephalopathy (L-BSE) is an atypical form of BSE that is transmissible to cattle and several lines of prion protein (PrP) transgenic mice, but not to wild-type mice. In this study, we examined the transmissibility of sheep-passaged L-BSE prions to wild-type mice. Disease-associated prion protein (PrP(Sc)) was detected in the brain and/or lymphoid tissues during the lifespan of mice that were asymptomatic subclinical carriers, indicating that wild-type mice were susceptible to sheep-passaged L-BSE. The morphological characteristics of the PrP(Sc) of sheep-passaged L-BSE included florid plaques that were distributed mainly in the cerebral cortex and hippocampus of subsequent passaged mice. The PrP(Sc) glycoform profiles of wild-type mice infected with sheep-passaged L-BSE were similar to those of the original isolate. The data indicate that sheep-passaged L-BSE has an altered host range and acquired transmissibility to wild-type mice.

No MeSH data available.


Related in: MedlinePlus