Limits...
Wnt affects symmetry and morphogenesis during post-embryonic development in colonial chordates.

Di Maio A, Setar L, Tiozzo S, De Tomaso AW - Evodevo (2015)

Bottom Line: Modulation of the Wnt signaling in either process has shown to result in unusual body axis phenotypes.Chemical manipulation of the pathway resulted in atypical budding due to the duplication of the A/P axes, supernumerary budding, and loss of the overall cell apical-basal polarity.Our results suggest that Wnt signaling is used for equivalent developmental processes both during embryogenesis and asexual development in an adult organism, suggesting that patterning mechanisms driving morphogenesis are conserved, independent of embryonic, or regenerative development.

View Article: PubMed Central - PubMed

Affiliation: School of Bioscience, University of Birmingham, Edgbaston, Birmingham, B19 2TT UK ; Molecular Cellular and Developmental Biology, University of California Santa Barbara, Santa Barbara, CA 93106 USA.

ABSTRACT

Background: Wnt signaling is one of the earliest and most highly conserved regulatory pathways for the establishment of the body axes during regeneration and early development. In regeneration, body axes determination occurs independently of tissue rearrangement and early developmental cues. Modulation of the Wnt signaling in either process has shown to result in unusual body axis phenotypes. Botryllus schlosseri is a colonial ascidian that can regenerate its entire body through asexual budding. This processes leads to an adult body via a stereotypical developmental pathway (called blastogenesis), without proceeding through any embryonic developmental stages.

Results: In this study, we describe the role of the canonical Wnt pathway during the early stages of asexual development. We characterized expression of three Wnt ligands (Wnt2B, Wnt5A, and Wnt9A) by in situ hybridization and qRT-PCR. Chemical manipulation of the pathway resulted in atypical budding due to the duplication of the A/P axes, supernumerary budding, and loss of the overall cell apical-basal polarity.

Conclusions: Our results suggest that Wnt signaling is used for equivalent developmental processes both during embryogenesis and asexual development in an adult organism, suggesting that patterning mechanisms driving morphogenesis are conserved, independent of embryonic, or regenerative development.

No MeSH data available.


Related in: MedlinePlus

Modification of the inner epithelial cells upon activation of Wnt. At the ultrastructural level, alteration of the Wnt pathway induces polarity changes of cells composing the thickening of the atrial epithelium of the inner vesicle of the primary bud. The normal pattern of cells oriented toward the outer side of the budlet (A) is mostly lost in drug-treated colonies (C). Particularly, in drug incubated animals (D), epithelial cells lose their nuclei orientation compared to the control animals (B). The collagen content (asterisks) in treated samples is thinner compared to the non-treated tissues. Shallow intercellular junctions (arrows) are visible in both preparations showing no structural differences. BAR: 10 micron (A and C); 1 micron (B and D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4499891&req=5

Fig7: Modification of the inner epithelial cells upon activation of Wnt. At the ultrastructural level, alteration of the Wnt pathway induces polarity changes of cells composing the thickening of the atrial epithelium of the inner vesicle of the primary bud. The normal pattern of cells oriented toward the outer side of the budlet (A) is mostly lost in drug-treated colonies (C). Particularly, in drug incubated animals (D), epithelial cells lose their nuclei orientation compared to the control animals (B). The collagen content (asterisks) in treated samples is thinner compared to the non-treated tissues. Shallow intercellular junctions (arrows) are visible in both preparations showing no structural differences. BAR: 10 micron (A and C); 1 micron (B and D).

Mentions: The initial stage of budlet development is a thickening of the peribranchial mono-layered epithelium of the primary bud, which evaginates, pushing out the overlaying epidermal epithelium, then pinches off to form a double layer vesicle [27]. At this stage, the inner layer folds into a sealed vesicle composed of thick cylindrical epithelial cells organized in a palisade layer. Cells are polarized showing a large nucleus located at the apical side, with a large nucleolus and mitochondria scattered in the cytoplasm (FigureĀ 7A and B) whereas cells in the external epithelium do not show this morphology.Figure 7


Wnt affects symmetry and morphogenesis during post-embryonic development in colonial chordates.

Di Maio A, Setar L, Tiozzo S, De Tomaso AW - Evodevo (2015)

Modification of the inner epithelial cells upon activation of Wnt. At the ultrastructural level, alteration of the Wnt pathway induces polarity changes of cells composing the thickening of the atrial epithelium of the inner vesicle of the primary bud. The normal pattern of cells oriented toward the outer side of the budlet (A) is mostly lost in drug-treated colonies (C). Particularly, in drug incubated animals (D), epithelial cells lose their nuclei orientation compared to the control animals (B). The collagen content (asterisks) in treated samples is thinner compared to the non-treated tissues. Shallow intercellular junctions (arrows) are visible in both preparations showing no structural differences. BAR: 10 micron (A and C); 1 micron (B and D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4499891&req=5

Fig7: Modification of the inner epithelial cells upon activation of Wnt. At the ultrastructural level, alteration of the Wnt pathway induces polarity changes of cells composing the thickening of the atrial epithelium of the inner vesicle of the primary bud. The normal pattern of cells oriented toward the outer side of the budlet (A) is mostly lost in drug-treated colonies (C). Particularly, in drug incubated animals (D), epithelial cells lose their nuclei orientation compared to the control animals (B). The collagen content (asterisks) in treated samples is thinner compared to the non-treated tissues. Shallow intercellular junctions (arrows) are visible in both preparations showing no structural differences. BAR: 10 micron (A and C); 1 micron (B and D).
Mentions: The initial stage of budlet development is a thickening of the peribranchial mono-layered epithelium of the primary bud, which evaginates, pushing out the overlaying epidermal epithelium, then pinches off to form a double layer vesicle [27]. At this stage, the inner layer folds into a sealed vesicle composed of thick cylindrical epithelial cells organized in a palisade layer. Cells are polarized showing a large nucleus located at the apical side, with a large nucleolus and mitochondria scattered in the cytoplasm (FigureĀ 7A and B) whereas cells in the external epithelium do not show this morphology.Figure 7

Bottom Line: Modulation of the Wnt signaling in either process has shown to result in unusual body axis phenotypes.Chemical manipulation of the pathway resulted in atypical budding due to the duplication of the A/P axes, supernumerary budding, and loss of the overall cell apical-basal polarity.Our results suggest that Wnt signaling is used for equivalent developmental processes both during embryogenesis and asexual development in an adult organism, suggesting that patterning mechanisms driving morphogenesis are conserved, independent of embryonic, or regenerative development.

View Article: PubMed Central - PubMed

Affiliation: School of Bioscience, University of Birmingham, Edgbaston, Birmingham, B19 2TT UK ; Molecular Cellular and Developmental Biology, University of California Santa Barbara, Santa Barbara, CA 93106 USA.

ABSTRACT

Background: Wnt signaling is one of the earliest and most highly conserved regulatory pathways for the establishment of the body axes during regeneration and early development. In regeneration, body axes determination occurs independently of tissue rearrangement and early developmental cues. Modulation of the Wnt signaling in either process has shown to result in unusual body axis phenotypes. Botryllus schlosseri is a colonial ascidian that can regenerate its entire body through asexual budding. This processes leads to an adult body via a stereotypical developmental pathway (called blastogenesis), without proceeding through any embryonic developmental stages.

Results: In this study, we describe the role of the canonical Wnt pathway during the early stages of asexual development. We characterized expression of three Wnt ligands (Wnt2B, Wnt5A, and Wnt9A) by in situ hybridization and qRT-PCR. Chemical manipulation of the pathway resulted in atypical budding due to the duplication of the A/P axes, supernumerary budding, and loss of the overall cell apical-basal polarity.

Conclusions: Our results suggest that Wnt signaling is used for equivalent developmental processes both during embryogenesis and asexual development in an adult organism, suggesting that patterning mechanisms driving morphogenesis are conserved, independent of embryonic, or regenerative development.

No MeSH data available.


Related in: MedlinePlus