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Honey reduces blood alcohol concentration but not affects the level of serum MDA and GSH-Px activity in intoxicated male mice models.

Shi P, Chen B, Chen C, Xu J, Shen Z, Miao X, Yao H - BMC Complement Altern Med (2015)

Bottom Line: Furthermore, the treatment of 2.19 g/kg honey could decrease significantly the blood ethanol concentration in intoxicated mice.The anti-intoxication activity of honey could be due to the effect of the fructose contained in the honey.Honey indeed possesses anti-intoxication activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine Resource and Bee products, College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

ABSTRACT

Background: For a long time, honey was purportedly helpful to prevent drunkenness and relieve hangover symptoms. However, few of the assertions have experienced scientific assessment. The present study examined the effects of honey on intoxicated male mice.

Methods: Low or high doses of lychee flower honey (2.19 or 4.39 g/kg body weight, respectively) were single orally administrated 30 min before the ethanol intoxication of mice, followed by recording the locomotor activity by autonomic activity instrument and observing the climbing ability after alcohol. On the other hand, 2.19 g/kg honey was single orally administrated 5 min after the ethanol intoxication of mice, followed by determining the ethanol concentration in mice blood. In addition, subacute alcoholism mice models were developed and after the treatment of 2.19 g/kg honey s.i.d for successive three days, the level of serum malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) activity were detected in the models.

Results: Both of the two doses of honey increased the autonomic activity of alcoholized mice. Furthermore, the treatment of 2.19 g/kg honey could decrease significantly the blood ethanol concentration in intoxicated mice. The anti-intoxication activity of honey could be due to the effect of the fructose contained in the honey. Meanwhile, honey could not affect the serum MDA level and GSH-Px activity in alcoholism mice models.

Conclusion: Honey indeed possesses anti-intoxication activity.

No MeSH data available.


Related in: MedlinePlus

The HPLC chromatograms of (a) mixture standard solutions including 4 mg/ml fructose, 3 mg/ml glucose and 1 mg/ml sucrose, and (b) honey samples (9.4 mg/ml). Conditions: High-performance liquid chromatographic analysis was carried out using a Shimadzu 20A series HPLC system (Japan) equipped with two pumps with column oven and ultraviolet detector; a Welch ultimate XB-NH2 column (4.6 mm × 250 mm, 5 μm) was used, sample injection volume was 20 μl, the temperature of column oven was set at 30 °C, flow rate was 1 ml/min, a isocratic elution was performed with mobile phases consisted of water and acetonitrile (30 : 70) and the monitored wavelength was set at 190 nm
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Fig2: The HPLC chromatograms of (a) mixture standard solutions including 4 mg/ml fructose, 3 mg/ml glucose and 1 mg/ml sucrose, and (b) honey samples (9.4 mg/ml). Conditions: High-performance liquid chromatographic analysis was carried out using a Shimadzu 20A series HPLC system (Japan) equipped with two pumps with column oven and ultraviolet detector; a Welch ultimate XB-NH2 column (4.6 mm × 250 mm, 5 μm) was used, sample injection volume was 20 μl, the temperature of column oven was set at 30 °C, flow rate was 1 ml/min, a isocratic elution was performed with mobile phases consisted of water and acetonitrile (30 : 70) and the monitored wavelength was set at 190 nm

Mentions: The results from the pretreatment of lychee flower honey showed that there is apparent preventing drunkenness effect in mice model for low dose of honey (2.19 g/kg BW) within 60 min after administration of alcohol. The prevention was also observed in mice for high dose of honey (4.39 g/kg BW) at 20 min and 40 min after alcohol. The results hinted that honey could be indeed helpful to prevent drunkenness. Since the major components contained in the studied honey are fructose (38.06 %, g/g) and glucose (32.67 %, g/g), which had been determined by a HPLC method (Fig. 2), it is reasonable to presume that the fructose or/and glucose resulted in the preventing drunkenness effect in alcoholized mice. The preventing effect in mice could be due to that fructose and/or glucose inhibited the absorption of ethanol in mice gastrointestinal tract or enhanced the elimination of ethanol in intoxicated mice. This hypothesis could obtain positive support from the observations that adult female rats, fed chow diets supplemented with fructose or glucose in their drinking water for 10 days demonstrated significantly greater ethanol elimination rates (4.85 ± 0.28 and 4.92 ± 1.5 μM ethanol/min/g liver, respectively) than rats receiving water (3.65 ± 0.29) [14]. In fact, it was also observed that the treatment of honey in alcoholized male mice could significantly reduce the blood alcohol concentration at 30 min after administration of honey in the present study. However, in the early clinical study, fructose infusions resulted in a lowering of the alcohol levels in the blood of man by 43 % compared to the values obtained during saline solution infusions and glucose infusions were ineffective [15]. It demonstrated that glucose could have no significant effect on the rate of ethanol elimination in men. A literature also reported that treatment with glucose was ineffective in reducing blood alcohol level in male rats with acute ethanol intoxication [16]. These reported results suggest that the subjects’ sex or genus could exert indeterminacy influence on honey (being rich in carbohydrates fructose and glucose) enhancing alcohol elimination or reducing blood alcohol concentration.Figure 2


Honey reduces blood alcohol concentration but not affects the level of serum MDA and GSH-Px activity in intoxicated male mice models.

Shi P, Chen B, Chen C, Xu J, Shen Z, Miao X, Yao H - BMC Complement Altern Med (2015)

The HPLC chromatograms of (a) mixture standard solutions including 4 mg/ml fructose, 3 mg/ml glucose and 1 mg/ml sucrose, and (b) honey samples (9.4 mg/ml). Conditions: High-performance liquid chromatographic analysis was carried out using a Shimadzu 20A series HPLC system (Japan) equipped with two pumps with column oven and ultraviolet detector; a Welch ultimate XB-NH2 column (4.6 mm × 250 mm, 5 μm) was used, sample injection volume was 20 μl, the temperature of column oven was set at 30 °C, flow rate was 1 ml/min, a isocratic elution was performed with mobile phases consisted of water and acetonitrile (30 : 70) and the monitored wavelength was set at 190 nm
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4499888&req=5

Fig2: The HPLC chromatograms of (a) mixture standard solutions including 4 mg/ml fructose, 3 mg/ml glucose and 1 mg/ml sucrose, and (b) honey samples (9.4 mg/ml). Conditions: High-performance liquid chromatographic analysis was carried out using a Shimadzu 20A series HPLC system (Japan) equipped with two pumps with column oven and ultraviolet detector; a Welch ultimate XB-NH2 column (4.6 mm × 250 mm, 5 μm) was used, sample injection volume was 20 μl, the temperature of column oven was set at 30 °C, flow rate was 1 ml/min, a isocratic elution was performed with mobile phases consisted of water and acetonitrile (30 : 70) and the monitored wavelength was set at 190 nm
Mentions: The results from the pretreatment of lychee flower honey showed that there is apparent preventing drunkenness effect in mice model for low dose of honey (2.19 g/kg BW) within 60 min after administration of alcohol. The prevention was also observed in mice for high dose of honey (4.39 g/kg BW) at 20 min and 40 min after alcohol. The results hinted that honey could be indeed helpful to prevent drunkenness. Since the major components contained in the studied honey are fructose (38.06 %, g/g) and glucose (32.67 %, g/g), which had been determined by a HPLC method (Fig. 2), it is reasonable to presume that the fructose or/and glucose resulted in the preventing drunkenness effect in alcoholized mice. The preventing effect in mice could be due to that fructose and/or glucose inhibited the absorption of ethanol in mice gastrointestinal tract or enhanced the elimination of ethanol in intoxicated mice. This hypothesis could obtain positive support from the observations that adult female rats, fed chow diets supplemented with fructose or glucose in their drinking water for 10 days demonstrated significantly greater ethanol elimination rates (4.85 ± 0.28 and 4.92 ± 1.5 μM ethanol/min/g liver, respectively) than rats receiving water (3.65 ± 0.29) [14]. In fact, it was also observed that the treatment of honey in alcoholized male mice could significantly reduce the blood alcohol concentration at 30 min after administration of honey in the present study. However, in the early clinical study, fructose infusions resulted in a lowering of the alcohol levels in the blood of man by 43 % compared to the values obtained during saline solution infusions and glucose infusions were ineffective [15]. It demonstrated that glucose could have no significant effect on the rate of ethanol elimination in men. A literature also reported that treatment with glucose was ineffective in reducing blood alcohol level in male rats with acute ethanol intoxication [16]. These reported results suggest that the subjects’ sex or genus could exert indeterminacy influence on honey (being rich in carbohydrates fructose and glucose) enhancing alcohol elimination or reducing blood alcohol concentration.Figure 2

Bottom Line: Furthermore, the treatment of 2.19 g/kg honey could decrease significantly the blood ethanol concentration in intoxicated mice.The anti-intoxication activity of honey could be due to the effect of the fructose contained in the honey.Honey indeed possesses anti-intoxication activity.

View Article: PubMed Central - PubMed

Affiliation: Department of Traditional Chinese Medicine Resource and Bee products, College of Bee Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

ABSTRACT

Background: For a long time, honey was purportedly helpful to prevent drunkenness and relieve hangover symptoms. However, few of the assertions have experienced scientific assessment. The present study examined the effects of honey on intoxicated male mice.

Methods: Low or high doses of lychee flower honey (2.19 or 4.39 g/kg body weight, respectively) were single orally administrated 30 min before the ethanol intoxication of mice, followed by recording the locomotor activity by autonomic activity instrument and observing the climbing ability after alcohol. On the other hand, 2.19 g/kg honey was single orally administrated 5 min after the ethanol intoxication of mice, followed by determining the ethanol concentration in mice blood. In addition, subacute alcoholism mice models were developed and after the treatment of 2.19 g/kg honey s.i.d for successive three days, the level of serum malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) activity were detected in the models.

Results: Both of the two doses of honey increased the autonomic activity of alcoholized mice. Furthermore, the treatment of 2.19 g/kg honey could decrease significantly the blood ethanol concentration in intoxicated mice. The anti-intoxication activity of honey could be due to the effect of the fructose contained in the honey. Meanwhile, honey could not affect the serum MDA level and GSH-Px activity in alcoholism mice models.

Conclusion: Honey indeed possesses anti-intoxication activity.

No MeSH data available.


Related in: MedlinePlus