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Radiofrequency treatment alters cancer cell phenotype.

Ware MJ, Tinger S, Colbert KL, Corr SJ, Rees P, Koshkina N, Curley S, Summers HD, Godin B - Sci Rep (2015)

Bottom Line: These characteristics are intrinsically different between malignant and non-malignant cells and change in response to therapy or in the progression of the disease.Our data show that cell topography, morphology, motility, adhesion and division change as a result of the treatment.Clear phenotypical differences were observed between cancerous and normal cells in both their untreated states and in their response to RF therapy.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Nanomedicine, Houston Methodist Research Institute, Houston, Texas, USA [2] Centre for Nanohealth, College of Engineering, Swansea University, Swansea, UK.

ABSTRACT
The importance of evaluating physical cues in cancer research is gradually being realized. Assessment of cancer cell physical appearance, or phenotype, may provide information on changes in cellular behavior, including migratory or communicative changes. These characteristics are intrinsically different between malignant and non-malignant cells and change in response to therapy or in the progression of the disease. Here, we report that pancreatic cancer cell phenotype was altered in response to a physical method for cancer therapy, a non-invasive radiofrequency (RF) treatment, which is currently being developed for human trials. We provide a battery of tests to explore these phenotype characteristics. Our data show that cell topography, morphology, motility, adhesion and division change as a result of the treatment. These may have consequences for tissue architecture, for diffusion of anti-cancer therapeutics and cancer cell susceptibility within the tumor. Clear phenotypical differences were observed between cancerous and normal cells in both their untreated states and in their response to RF therapy. We also report, for the first time, a transfer of microsized particles through tunneling nanotubes, which were produced by cancer cells in response to RF therapy. Additionally, we provide evidence that various sub-populations of cancer cells heterogeneously respond to RF treatment.

No MeSH data available.


Related in: MedlinePlus

Cell Proliferation in adhered population.WST assay quantified metabolic activity/proliferation in adhered and non-adhered a) AsPc-1, b) PANC-1 and c) HPDE cells (% proliferation normalized to cell numbers before RF treatment). d) Trypan Blue assay quantified the total number of RF treated cells at 48 h. Normalized to the number of cells in untreated plates at the same time point (normalized to number of untreated cells).
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f6: Cell Proliferation in adhered population.WST assay quantified metabolic activity/proliferation in adhered and non-adhered a) AsPc-1, b) PANC-1 and c) HPDE cells (% proliferation normalized to cell numbers before RF treatment). d) Trypan Blue assay quantified the total number of RF treated cells at 48 h. Normalized to the number of cells in untreated plates at the same time point (normalized to number of untreated cells).

Mentions: Previous studies have shown that the use of noninvasive RF fields decreases viability in human and mammalian pancreatic cancer cells with limited effect on non-malignant cells3. The WST assay provides an indirect method for the quantification of proliferative activities within a cell population containing both adhered and non-adhered cells. It must be noted that cell number or metabolic activity cannot be extrapolated as a single measurement from the results of the WST assay. A decrease in absorbance indicates both a decrease in proliferation and/or a decrease in metabolism in PANC-1 and AsPc-1 cell lines (Fig. 6a). HPDE displayed a less drastic effect (Fig. 6a). To obtain a more direct measure of proliferation rate, a cell count at 48 h after RF treatment was carried out using the Trypan blue assay (Fig. 6b). These data suggests that RF disrupts the metabolic activity and proliferation in all cell lines, however HPDE showed a much less drastic response. Additionally, the non-adhered population was singled out for study and were recovered from the supernatant after a single RF treatment and reseeded in fresh media. The non-adhered cells were able to re-establish a cell population, however they also showed decreased proliferation rate between 0 and 1 week after reseeding when compared to untreated cells.


Radiofrequency treatment alters cancer cell phenotype.

Ware MJ, Tinger S, Colbert KL, Corr SJ, Rees P, Koshkina N, Curley S, Summers HD, Godin B - Sci Rep (2015)

Cell Proliferation in adhered population.WST assay quantified metabolic activity/proliferation in adhered and non-adhered a) AsPc-1, b) PANC-1 and c) HPDE cells (% proliferation normalized to cell numbers before RF treatment). d) Trypan Blue assay quantified the total number of RF treated cells at 48 h. Normalized to the number of cells in untreated plates at the same time point (normalized to number of untreated cells).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499808&req=5

f6: Cell Proliferation in adhered population.WST assay quantified metabolic activity/proliferation in adhered and non-adhered a) AsPc-1, b) PANC-1 and c) HPDE cells (% proliferation normalized to cell numbers before RF treatment). d) Trypan Blue assay quantified the total number of RF treated cells at 48 h. Normalized to the number of cells in untreated plates at the same time point (normalized to number of untreated cells).
Mentions: Previous studies have shown that the use of noninvasive RF fields decreases viability in human and mammalian pancreatic cancer cells with limited effect on non-malignant cells3. The WST assay provides an indirect method for the quantification of proliferative activities within a cell population containing both adhered and non-adhered cells. It must be noted that cell number or metabolic activity cannot be extrapolated as a single measurement from the results of the WST assay. A decrease in absorbance indicates both a decrease in proliferation and/or a decrease in metabolism in PANC-1 and AsPc-1 cell lines (Fig. 6a). HPDE displayed a less drastic effect (Fig. 6a). To obtain a more direct measure of proliferation rate, a cell count at 48 h after RF treatment was carried out using the Trypan blue assay (Fig. 6b). These data suggests that RF disrupts the metabolic activity and proliferation in all cell lines, however HPDE showed a much less drastic response. Additionally, the non-adhered population was singled out for study and were recovered from the supernatant after a single RF treatment and reseeded in fresh media. The non-adhered cells were able to re-establish a cell population, however they also showed decreased proliferation rate between 0 and 1 week after reseeding when compared to untreated cells.

Bottom Line: These characteristics are intrinsically different between malignant and non-malignant cells and change in response to therapy or in the progression of the disease.Our data show that cell topography, morphology, motility, adhesion and division change as a result of the treatment.Clear phenotypical differences were observed between cancerous and normal cells in both their untreated states and in their response to RF therapy.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Nanomedicine, Houston Methodist Research Institute, Houston, Texas, USA [2] Centre for Nanohealth, College of Engineering, Swansea University, Swansea, UK.

ABSTRACT
The importance of evaluating physical cues in cancer research is gradually being realized. Assessment of cancer cell physical appearance, or phenotype, may provide information on changes in cellular behavior, including migratory or communicative changes. These characteristics are intrinsically different between malignant and non-malignant cells and change in response to therapy or in the progression of the disease. Here, we report that pancreatic cancer cell phenotype was altered in response to a physical method for cancer therapy, a non-invasive radiofrequency (RF) treatment, which is currently being developed for human trials. We provide a battery of tests to explore these phenotype characteristics. Our data show that cell topography, morphology, motility, adhesion and division change as a result of the treatment. These may have consequences for tissue architecture, for diffusion of anti-cancer therapeutics and cancer cell susceptibility within the tumor. Clear phenotypical differences were observed between cancerous and normal cells in both their untreated states and in their response to RF therapy. We also report, for the first time, a transfer of microsized particles through tunneling nanotubes, which were produced by cancer cells in response to RF therapy. Additionally, we provide evidence that various sub-populations of cancer cells heterogeneously respond to RF treatment.

No MeSH data available.


Related in: MedlinePlus