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Radiofrequency treatment alters cancer cell phenotype.

Ware MJ, Tinger S, Colbert KL, Corr SJ, Rees P, Koshkina N, Curley S, Summers HD, Godin B - Sci Rep (2015)

Bottom Line: These characteristics are intrinsically different between malignant and non-malignant cells and change in response to therapy or in the progression of the disease.Our data show that cell topography, morphology, motility, adhesion and division change as a result of the treatment.Clear phenotypical differences were observed between cancerous and normal cells in both their untreated states and in their response to RF therapy.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Nanomedicine, Houston Methodist Research Institute, Houston, Texas, USA [2] Centre for Nanohealth, College of Engineering, Swansea University, Swansea, UK.

ABSTRACT
The importance of evaluating physical cues in cancer research is gradually being realized. Assessment of cancer cell physical appearance, or phenotype, may provide information on changes in cellular behavior, including migratory or communicative changes. These characteristics are intrinsically different between malignant and non-malignant cells and change in response to therapy or in the progression of the disease. Here, we report that pancreatic cancer cell phenotype was altered in response to a physical method for cancer therapy, a non-invasive radiofrequency (RF) treatment, which is currently being developed for human trials. We provide a battery of tests to explore these phenotype characteristics. Our data show that cell topography, morphology, motility, adhesion and division change as a result of the treatment. These may have consequences for tissue architecture, for diffusion of anti-cancer therapeutics and cancer cell susceptibility within the tumor. Clear phenotypical differences were observed between cancerous and normal cells in both their untreated states and in their response to RF therapy. We also report, for the first time, a transfer of microsized particles through tunneling nanotubes, which were produced by cancer cells in response to RF therapy. Additionally, we provide evidence that various sub-populations of cancer cells heterogeneously respond to RF treatment.

No MeSH data available.


Related in: MedlinePlus

Cell motility in response to RF treatment.a) Cell tracking via co-ordinates over 20 h before and after RF. b) 2-D motility measured for 20 h before RF treatment and between 4 and 24 h after RF treatment (% motility normalized to motility before RF). c) F-Actin expression before and after RF (n = 81 frames), d) Cell-substrate unattachment in response to RF treatment, the number of live and dead cells in un-adhered subpopulation after RF treatment and e) 3D migratory behavior of cancer cells before and after a single RF treatment (*p < 0.01).
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f3: Cell motility in response to RF treatment.a) Cell tracking via co-ordinates over 20 h before and after RF. b) 2-D motility measured for 20 h before RF treatment and between 4 and 24 h after RF treatment (% motility normalized to motility before RF). c) F-Actin expression before and after RF (n = 81 frames), d) Cell-substrate unattachment in response to RF treatment, the number of live and dead cells in un-adhered subpopulation after RF treatment and e) 3D migratory behavior of cancer cells before and after a single RF treatment (*p < 0.01).

Mentions: Cell motility is influential in various pathological processes, such as cancer metastasis, and has therefore generated recent interest1718. Cell motility is known to involve continuous mechanosensation19, that may be altered by the RF field. Cell motility before and after RF treatment in the sub-population of cells still adhered to the substrate was evaluated in the 2D environment. Motility was measured using ImageJ software by manually selecting the center of mass of a cell and following it through a 20 h time-period by recording its XY co-ordinates at each timepoint (Fig. 3a). These measures revealed that regulation of cell speed and maximum displacement over 20 hours were dependent on RF exposure. Between 4–24 h after RF treatment the PANC-1 and AsPc-1 cells, which remained adhered to the substrate, displayed approximately a 20% increase in maximal displacement, (Fig. 3b). Increases in motility may be due to stress; it is well known cancer cells are more sensitive to hyperthermia20 which has been attributed to their dielectric properties4. RF treatment caused the motility in HPDE cells to decrease in the 20 hours following RF treatment.


Radiofrequency treatment alters cancer cell phenotype.

Ware MJ, Tinger S, Colbert KL, Corr SJ, Rees P, Koshkina N, Curley S, Summers HD, Godin B - Sci Rep (2015)

Cell motility in response to RF treatment.a) Cell tracking via co-ordinates over 20 h before and after RF. b) 2-D motility measured for 20 h before RF treatment and between 4 and 24 h after RF treatment (% motility normalized to motility before RF). c) F-Actin expression before and after RF (n = 81 frames), d) Cell-substrate unattachment in response to RF treatment, the number of live and dead cells in un-adhered subpopulation after RF treatment and e) 3D migratory behavior of cancer cells before and after a single RF treatment (*p < 0.01).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499808&req=5

f3: Cell motility in response to RF treatment.a) Cell tracking via co-ordinates over 20 h before and after RF. b) 2-D motility measured for 20 h before RF treatment and between 4 and 24 h after RF treatment (% motility normalized to motility before RF). c) F-Actin expression before and after RF (n = 81 frames), d) Cell-substrate unattachment in response to RF treatment, the number of live and dead cells in un-adhered subpopulation after RF treatment and e) 3D migratory behavior of cancer cells before and after a single RF treatment (*p < 0.01).
Mentions: Cell motility is influential in various pathological processes, such as cancer metastasis, and has therefore generated recent interest1718. Cell motility is known to involve continuous mechanosensation19, that may be altered by the RF field. Cell motility before and after RF treatment in the sub-population of cells still adhered to the substrate was evaluated in the 2D environment. Motility was measured using ImageJ software by manually selecting the center of mass of a cell and following it through a 20 h time-period by recording its XY co-ordinates at each timepoint (Fig. 3a). These measures revealed that regulation of cell speed and maximum displacement over 20 hours were dependent on RF exposure. Between 4–24 h after RF treatment the PANC-1 and AsPc-1 cells, which remained adhered to the substrate, displayed approximately a 20% increase in maximal displacement, (Fig. 3b). Increases in motility may be due to stress; it is well known cancer cells are more sensitive to hyperthermia20 which has been attributed to their dielectric properties4. RF treatment caused the motility in HPDE cells to decrease in the 20 hours following RF treatment.

Bottom Line: These characteristics are intrinsically different between malignant and non-malignant cells and change in response to therapy or in the progression of the disease.Our data show that cell topography, morphology, motility, adhesion and division change as a result of the treatment.Clear phenotypical differences were observed between cancerous and normal cells in both their untreated states and in their response to RF therapy.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Nanomedicine, Houston Methodist Research Institute, Houston, Texas, USA [2] Centre for Nanohealth, College of Engineering, Swansea University, Swansea, UK.

ABSTRACT
The importance of evaluating physical cues in cancer research is gradually being realized. Assessment of cancer cell physical appearance, or phenotype, may provide information on changes in cellular behavior, including migratory or communicative changes. These characteristics are intrinsically different between malignant and non-malignant cells and change in response to therapy or in the progression of the disease. Here, we report that pancreatic cancer cell phenotype was altered in response to a physical method for cancer therapy, a non-invasive radiofrequency (RF) treatment, which is currently being developed for human trials. We provide a battery of tests to explore these phenotype characteristics. Our data show that cell topography, morphology, motility, adhesion and division change as a result of the treatment. These may have consequences for tissue architecture, for diffusion of anti-cancer therapeutics and cancer cell susceptibility within the tumor. Clear phenotypical differences were observed between cancerous and normal cells in both their untreated states and in their response to RF therapy. We also report, for the first time, a transfer of microsized particles through tunneling nanotubes, which were produced by cancer cells in response to RF therapy. Additionally, we provide evidence that various sub-populations of cancer cells heterogeneously respond to RF treatment.

No MeSH data available.


Related in: MedlinePlus