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Identification of common horsetail (Equisetum arvense L.; Equisetaceae) using Thin Layer Chromatography versus DNA barcoding.

Saslis-Lagoudakis CH, Bruun-Lund S, Iwanycki NE, Seberg O, Petersen G, Jäger AK, Rønsted N - Sci Rep (2015)

Bottom Line: We find that both methods can discriminate between the two species and positively identify E. arvense.The TLC-test is more cost- and time-efficient, but DNA barcoding is more powerful in determining the identity of adulterant species.Our study shows that, although DNA barcoding presents certain advantages, other established laboratory methods can perform as well or even better in confirming species' identity in herbal products.

View Article: PubMed Central - PubMed

Affiliation: Evolutionary Genomics Section, Natural History Museum of Denmark, Sølvgade 83S, Copenhagen, DK-1307, Denmark.

ABSTRACT
The global herbal products market has grown in recent years, making regulation of these products paramount for public healthcare. For instance, the common horsetail (Equisetum arvense L.) is used in numerous herbal products, but it can be adulterated with closely related species, especially E. palustre L. that can produce toxic alkaloids. As morphology-based identification is often difficult or impossible, the identification of processed material can be aided by molecular techniques. In this study, we explore two molecular identification techniques as methods of testing the purity of these products: a Thin Layer Chromatography approach (TLC-test) included in the European Pharmacopoeia and a DNA barcoding approach, used in recent years to identify material in herbal products. We test the potential of these methods for distinguishing and identifying these species using material from herbarium collections and commercial herbal products. We find that both methods can discriminate between the two species and positively identify E. arvense. The TLC-test is more cost- and time-efficient, but DNA barcoding is more powerful in determining the identity of adulterant species. Our study shows that, although DNA barcoding presents certain advantages, other established laboratory methods can perform as well or even better in confirming species' identity in herbal products.

No MeSH data available.


Phylogeny of Equisetum reconstructed with a Maximum Likelihood analysis based on five DNA markers (ITS2, matK, rbcL, rps4, trnH-psbA).Bootstrap support values are given above respective branches.
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f1: Phylogeny of Equisetum reconstructed with a Maximum Likelihood analysis based on five DNA markers (ITS2, matK, rbcL, rps4, trnH-psbA).Bootstrap support values are given above respective branches.

Mentions: We reconstructed a phylogenetic tree of Equisetum (Fig. 1) in order to test the monophyly of the species. Previous studies have provided phylogenetic hypotheses for the genus using plastid DNA markers353637, but these studies only included one specimen per species. The topology obtained here from nuclear and plastid markers, and including several accessions per species, largely corresponds to the topology found previously353637. Equisetum bogotense Kunth is recovered as sister to the rest of the genus and not as a member of subg. Hippochaete (Milde) Baker. The remainder of the genus is resolved into two major clades, each comprising seven species and corresponding to the two subgenera Equisetum and Hippochaete (Fig. 1). With the exception of E. diffusum D. Don and E. sylvaticum, all species were recovered as monophyletic, including E. arvense and E. palustre. These two species are resolved in the same clade (subg. Equisetum), but not as sister species (Fig. 1).


Identification of common horsetail (Equisetum arvense L.; Equisetaceae) using Thin Layer Chromatography versus DNA barcoding.

Saslis-Lagoudakis CH, Bruun-Lund S, Iwanycki NE, Seberg O, Petersen G, Jäger AK, Rønsted N - Sci Rep (2015)

Phylogeny of Equisetum reconstructed with a Maximum Likelihood analysis based on five DNA markers (ITS2, matK, rbcL, rps4, trnH-psbA).Bootstrap support values are given above respective branches.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499799&req=5

f1: Phylogeny of Equisetum reconstructed with a Maximum Likelihood analysis based on five DNA markers (ITS2, matK, rbcL, rps4, trnH-psbA).Bootstrap support values are given above respective branches.
Mentions: We reconstructed a phylogenetic tree of Equisetum (Fig. 1) in order to test the monophyly of the species. Previous studies have provided phylogenetic hypotheses for the genus using plastid DNA markers353637, but these studies only included one specimen per species. The topology obtained here from nuclear and plastid markers, and including several accessions per species, largely corresponds to the topology found previously353637. Equisetum bogotense Kunth is recovered as sister to the rest of the genus and not as a member of subg. Hippochaete (Milde) Baker. The remainder of the genus is resolved into two major clades, each comprising seven species and corresponding to the two subgenera Equisetum and Hippochaete (Fig. 1). With the exception of E. diffusum D. Don and E. sylvaticum, all species were recovered as monophyletic, including E. arvense and E. palustre. These two species are resolved in the same clade (subg. Equisetum), but not as sister species (Fig. 1).

Bottom Line: We find that both methods can discriminate between the two species and positively identify E. arvense.The TLC-test is more cost- and time-efficient, but DNA barcoding is more powerful in determining the identity of adulterant species.Our study shows that, although DNA barcoding presents certain advantages, other established laboratory methods can perform as well or even better in confirming species' identity in herbal products.

View Article: PubMed Central - PubMed

Affiliation: Evolutionary Genomics Section, Natural History Museum of Denmark, Sølvgade 83S, Copenhagen, DK-1307, Denmark.

ABSTRACT
The global herbal products market has grown in recent years, making regulation of these products paramount for public healthcare. For instance, the common horsetail (Equisetum arvense L.) is used in numerous herbal products, but it can be adulterated with closely related species, especially E. palustre L. that can produce toxic alkaloids. As morphology-based identification is often difficult or impossible, the identification of processed material can be aided by molecular techniques. In this study, we explore two molecular identification techniques as methods of testing the purity of these products: a Thin Layer Chromatography approach (TLC-test) included in the European Pharmacopoeia and a DNA barcoding approach, used in recent years to identify material in herbal products. We test the potential of these methods for distinguishing and identifying these species using material from herbarium collections and commercial herbal products. We find that both methods can discriminate between the two species and positively identify E. arvense. The TLC-test is more cost- and time-efficient, but DNA barcoding is more powerful in determining the identity of adulterant species. Our study shows that, although DNA barcoding presents certain advantages, other established laboratory methods can perform as well or even better in confirming species' identity in herbal products.

No MeSH data available.