Limits...
Synaptic representation of locomotion in single cerebellar granule cells.

Powell K, Mathy A, Duguid I, Häusser M - Elife (2015)

Bottom Line: Here, we use in vivo patch-clamp recordings to show that locomotion can be directly read out from mossy fiber synaptic input and spike output in single granule cells.The increase in granule cell spiking during locomotion is enhanced by glutamate spillover currents recruited during movement.Thus, synaptic input delivers remarkably rich information to single neurons during locomotion.

View Article: PubMed Central - PubMed

Affiliation: Wolfson Institute for Biomedical Research and Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom.

ABSTRACT
The cerebellum plays a crucial role in the regulation of locomotion, but how movement is represented at the synaptic level is not known. Here, we use in vivo patch-clamp recordings to show that locomotion can be directly read out from mossy fiber synaptic input and spike output in single granule cells. The increase in granule cell spiking during locomotion is enhanced by glutamate spillover currents recruited during movement. Surprisingly, the entire step sequence can be predicted from input EPSCs and output spikes of a single granule cell, suggesting that a robust gait code is present already at the cerebellar input layer and transmitted via the granule cell pathway to downstream Purkinje cells. Thus, synaptic input delivers remarkably rich information to single neurons during locomotion.

No MeSH data available.


Related in: MedlinePlus

Relationship between burst depolarization and burst frequency. Each colour represents a different cell from which the burst was recorded.DOI:http://dx.doi.org/10.7554/eLife.07290.016
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4499793&req=5

fig7: Relationship between burst depolarization and burst frequency. Each colour represents a different cell from which the burst was recorded.DOI:http://dx.doi.org/10.7554/eLife.07290.016

Mentions: We apologize for this typo and have fixed the text and legend. The P-value is indeed across the bursts, not cells. We have also made the text more precise: out of the 7 cells, 4 had bursts, adding up to 37 bursts in total. The figure below relates the burst depolarization with the burst frequency—there is a good correlation (Author response image 3). There are too few bursts per cell to comment on whether this population trend is also visible at the single cell level.10.7554/eLife.07290.016Author response image 3.


Synaptic representation of locomotion in single cerebellar granule cells.

Powell K, Mathy A, Duguid I, Häusser M - Elife (2015)

Relationship between burst depolarization and burst frequency. Each colour represents a different cell from which the burst was recorded.DOI:http://dx.doi.org/10.7554/eLife.07290.016
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499793&req=5

fig7: Relationship between burst depolarization and burst frequency. Each colour represents a different cell from which the burst was recorded.DOI:http://dx.doi.org/10.7554/eLife.07290.016
Mentions: We apologize for this typo and have fixed the text and legend. The P-value is indeed across the bursts, not cells. We have also made the text more precise: out of the 7 cells, 4 had bursts, adding up to 37 bursts in total. The figure below relates the burst depolarization with the burst frequency—there is a good correlation (Author response image 3). There are too few bursts per cell to comment on whether this population trend is also visible at the single cell level.10.7554/eLife.07290.016Author response image 3.

Bottom Line: Here, we use in vivo patch-clamp recordings to show that locomotion can be directly read out from mossy fiber synaptic input and spike output in single granule cells.The increase in granule cell spiking during locomotion is enhanced by glutamate spillover currents recruited during movement.Thus, synaptic input delivers remarkably rich information to single neurons during locomotion.

View Article: PubMed Central - PubMed

Affiliation: Wolfson Institute for Biomedical Research and Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom.

ABSTRACT
The cerebellum plays a crucial role in the regulation of locomotion, but how movement is represented at the synaptic level is not known. Here, we use in vivo patch-clamp recordings to show that locomotion can be directly read out from mossy fiber synaptic input and spike output in single granule cells. The increase in granule cell spiking during locomotion is enhanced by glutamate spillover currents recruited during movement. Surprisingly, the entire step sequence can be predicted from input EPSCs and output spikes of a single granule cell, suggesting that a robust gait code is present already at the cerebellar input layer and transmitted via the granule cell pathway to downstream Purkinje cells. Thus, synaptic input delivers remarkably rich information to single neurons during locomotion.

No MeSH data available.


Related in: MedlinePlus