Limits...
Isolation and characterization of an osmotic stress and ABA induced histone deacetylase in Arachis hygogaea.

Su LC, Deng B, Liu S, Li LM, Hu B, Zhong YT, Li L - Front Plant Sci (2015)

Bottom Line: Using RNA-seq data for peanut, we found a RPD3/HDA1-like superfamily histone deacetylase (HDAC), termed AhHDA1, whose gene is up-regulated by PEG-induced water limitation and ABA signaling.To understand whether and how osmotic stress and ABA mediate the peanut stress response by epigenetics, the expression of AhHDA1 and stress-responsive genes following treatment with PEG, ABA, and the specific HDAC inhibitor trichostatin A (TSA) were analyzed.AhHDA1 transcript levels were enhanced by all three treatments, as was expression of peanut transcription factor genes, indicating that AhHDA1 might be involved in the epigenetic regulation of stress resistance genes that comprise the responses to osmotic stress and ABA.

View Article: PubMed Central - PubMed

Affiliation: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University Guangzhou, China.

ABSTRACT
Histone acetylation, which together with histone methylation regulates gene activity in response to stress, is an important epigenetic modification. There is an increasing research focus on histone acetylation in crops, but there is no information to date in peanut (Arachis hypogaea). We showed that osmotic stress and ABA affect the acetylation of histone H3 loci in peanut seedlings by immunoblotting experiments. Using RNA-seq data for peanut, we found a RPD3/HDA1-like superfamily histone deacetylase (HDAC), termed AhHDA1, whose gene is up-regulated by PEG-induced water limitation and ABA signaling. We isolated and characterized AhHDA1 from A. hypogaea, showing that AhHDA1 is very similar to an Arabidopsis HDAC (AtHDA6) and, in recombinant form, possesses HDAC activity. To understand whether and how osmotic stress and ABA mediate the peanut stress response by epigenetics, the expression of AhHDA1 and stress-responsive genes following treatment with PEG, ABA, and the specific HDAC inhibitor trichostatin A (TSA) were analyzed. AhHDA1 transcript levels were enhanced by all three treatments, as was expression of peanut transcription factor genes, indicating that AhHDA1 might be involved in the epigenetic regulation of stress resistance genes that comprise the responses to osmotic stress and ABA.

No MeSH data available.


Expression analyses ofAhHDA1and stress resistance genes following TSA treatment by qRT-PCR. Time points of 1, 2, 5, and 8 h were sampled to observe the changing trend. The untreated group was used as the control (no chemical treatment). Each graph shows the mean and SD of three independent experiments. */**, different from control as revealed by t-test, p < 0.0.5/p < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4499716&req=5

Figure 6: Expression analyses ofAhHDA1and stress resistance genes following TSA treatment by qRT-PCR. Time points of 1, 2, 5, and 8 h were sampled to observe the changing trend. The untreated group was used as the control (no chemical treatment). Each graph shows the mean and SD of three independent experiments. */**, different from control as revealed by t-test, p < 0.0.5/p < 0.01.

Mentions: TSA is an HDAC inhibitor that induces transient hyperacetylation of histones H2B, H4, and H3 (Waterborg, 2011). Drought-induced RAB18, RD29B, HSP70 and four late embryogenesis abundant protein genes (LEA) are up-regulated by TSA in imbibing A. thaliana seeds (Tai et al., 2005). In our studies, TSA promoted the expression of AhHDA1 (Figure 6) and induced acetylation of H3 (Figure S4). The expression patterns of three TF genes (AhAREB1, AhDREB2A-like, and AhWRKY33-like) and two functional genes (AhDHN2 and AhNCED1) were also determined to study how TSA acted on stress resistance genes. TF gene expression was found to increase at an early time point after TSA treatment, then decreased as time went on. Although the expression profile of the functional genes was similar, the transcript level of these genes remained high relative to the control groups.


Isolation and characterization of an osmotic stress and ABA induced histone deacetylase in Arachis hygogaea.

Su LC, Deng B, Liu S, Li LM, Hu B, Zhong YT, Li L - Front Plant Sci (2015)

Expression analyses ofAhHDA1and stress resistance genes following TSA treatment by qRT-PCR. Time points of 1, 2, 5, and 8 h were sampled to observe the changing trend. The untreated group was used as the control (no chemical treatment). Each graph shows the mean and SD of three independent experiments. */**, different from control as revealed by t-test, p < 0.0.5/p < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499716&req=5

Figure 6: Expression analyses ofAhHDA1and stress resistance genes following TSA treatment by qRT-PCR. Time points of 1, 2, 5, and 8 h were sampled to observe the changing trend. The untreated group was used as the control (no chemical treatment). Each graph shows the mean and SD of three independent experiments. */**, different from control as revealed by t-test, p < 0.0.5/p < 0.01.
Mentions: TSA is an HDAC inhibitor that induces transient hyperacetylation of histones H2B, H4, and H3 (Waterborg, 2011). Drought-induced RAB18, RD29B, HSP70 and four late embryogenesis abundant protein genes (LEA) are up-regulated by TSA in imbibing A. thaliana seeds (Tai et al., 2005). In our studies, TSA promoted the expression of AhHDA1 (Figure 6) and induced acetylation of H3 (Figure S4). The expression patterns of three TF genes (AhAREB1, AhDREB2A-like, and AhWRKY33-like) and two functional genes (AhDHN2 and AhNCED1) were also determined to study how TSA acted on stress resistance genes. TF gene expression was found to increase at an early time point after TSA treatment, then decreased as time went on. Although the expression profile of the functional genes was similar, the transcript level of these genes remained high relative to the control groups.

Bottom Line: Using RNA-seq data for peanut, we found a RPD3/HDA1-like superfamily histone deacetylase (HDAC), termed AhHDA1, whose gene is up-regulated by PEG-induced water limitation and ABA signaling.To understand whether and how osmotic stress and ABA mediate the peanut stress response by epigenetics, the expression of AhHDA1 and stress-responsive genes following treatment with PEG, ABA, and the specific HDAC inhibitor trichostatin A (TSA) were analyzed.AhHDA1 transcript levels were enhanced by all three treatments, as was expression of peanut transcription factor genes, indicating that AhHDA1 might be involved in the epigenetic regulation of stress resistance genes that comprise the responses to osmotic stress and ABA.

View Article: PubMed Central - PubMed

Affiliation: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University Guangzhou, China.

ABSTRACT
Histone acetylation, which together with histone methylation regulates gene activity in response to stress, is an important epigenetic modification. There is an increasing research focus on histone acetylation in crops, but there is no information to date in peanut (Arachis hypogaea). We showed that osmotic stress and ABA affect the acetylation of histone H3 loci in peanut seedlings by immunoblotting experiments. Using RNA-seq data for peanut, we found a RPD3/HDA1-like superfamily histone deacetylase (HDAC), termed AhHDA1, whose gene is up-regulated by PEG-induced water limitation and ABA signaling. We isolated and characterized AhHDA1 from A. hypogaea, showing that AhHDA1 is very similar to an Arabidopsis HDAC (AtHDA6) and, in recombinant form, possesses HDAC activity. To understand whether and how osmotic stress and ABA mediate the peanut stress response by epigenetics, the expression of AhHDA1 and stress-responsive genes following treatment with PEG, ABA, and the specific HDAC inhibitor trichostatin A (TSA) were analyzed. AhHDA1 transcript levels were enhanced by all three treatments, as was expression of peanut transcription factor genes, indicating that AhHDA1 might be involved in the epigenetic regulation of stress resistance genes that comprise the responses to osmotic stress and ABA.

No MeSH data available.