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Isolation and characterization of an osmotic stress and ABA induced histone deacetylase in Arachis hygogaea.

Su LC, Deng B, Liu S, Li LM, Hu B, Zhong YT, Li L - Front Plant Sci (2015)

Bottom Line: Using RNA-seq data for peanut, we found a RPD3/HDA1-like superfamily histone deacetylase (HDAC), termed AhHDA1, whose gene is up-regulated by PEG-induced water limitation and ABA signaling.To understand whether and how osmotic stress and ABA mediate the peanut stress response by epigenetics, the expression of AhHDA1 and stress-responsive genes following treatment with PEG, ABA, and the specific HDAC inhibitor trichostatin A (TSA) were analyzed.AhHDA1 transcript levels were enhanced by all three treatments, as was expression of peanut transcription factor genes, indicating that AhHDA1 might be involved in the epigenetic regulation of stress resistance genes that comprise the responses to osmotic stress and ABA.

View Article: PubMed Central - PubMed

Affiliation: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University Guangzhou, China.

ABSTRACT
Histone acetylation, which together with histone methylation regulates gene activity in response to stress, is an important epigenetic modification. There is an increasing research focus on histone acetylation in crops, but there is no information to date in peanut (Arachis hypogaea). We showed that osmotic stress and ABA affect the acetylation of histone H3 loci in peanut seedlings by immunoblotting experiments. Using RNA-seq data for peanut, we found a RPD3/HDA1-like superfamily histone deacetylase (HDAC), termed AhHDA1, whose gene is up-regulated by PEG-induced water limitation and ABA signaling. We isolated and characterized AhHDA1 from A. hypogaea, showing that AhHDA1 is very similar to an Arabidopsis HDAC (AtHDA6) and, in recombinant form, possesses HDAC activity. To understand whether and how osmotic stress and ABA mediate the peanut stress response by epigenetics, the expression of AhHDA1 and stress-responsive genes following treatment with PEG, ABA, and the specific HDAC inhibitor trichostatin A (TSA) were analyzed. AhHDA1 transcript levels were enhanced by all three treatments, as was expression of peanut transcription factor genes, indicating that AhHDA1 might be involved in the epigenetic regulation of stress resistance genes that comprise the responses to osmotic stress and ABA.

No MeSH data available.


Expression analyses ofAhHDA1and stress resistance genes following PEG treatment by qRT-PCR. Time points of 1, 2, 5, and 8 h were sampled to observe the changing trend. The untreated group was used as the control (no chemical treatment). Each graph shows the mean and SD of three independent experiments. */**, different from control as revealed by t-test, p < 0.0.5/p < 0.01.
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Figure 5: Expression analyses ofAhHDA1and stress resistance genes following PEG treatment by qRT-PCR. Time points of 1, 2, 5, and 8 h were sampled to observe the changing trend. The untreated group was used as the control (no chemical treatment). Each graph shows the mean and SD of three independent experiments. */**, different from control as revealed by t-test, p < 0.0.5/p < 0.01.

Mentions: The effect of osmotic stress and exogenous ABA function on AhHDA1 was examined and it was found to be up-regulated by both PEG and ABA early in the response to both treatments (Figures 4, 5). However, the mechanisms underlying these responses are not known, and therefore it is not clear whether ABA and osmotic stress act on AhHDA1 via a common pathway or via independent pathways. Given that AhHDA1 transcription begins to increase significantly after 1 h of ABA treatment, but not until 2 h after PEG treatment, and that H3K14ac increases from 1 h of ABA treatment, while H3K9ac increases from 2 h of PEG treatment, it is possible that ABA-dependent stress-responsive genes are activated through modification of the H3K14 locus, and ABA-independent stress-responsive genes are activated at the H3K9 locus. The increased AhHDA1 expression induced by PEG or ABA might result from rapid changes in the HDAC and HAT “switches,” which re-balance histone and deacetylation.


Isolation and characterization of an osmotic stress and ABA induced histone deacetylase in Arachis hygogaea.

Su LC, Deng B, Liu S, Li LM, Hu B, Zhong YT, Li L - Front Plant Sci (2015)

Expression analyses ofAhHDA1and stress resistance genes following PEG treatment by qRT-PCR. Time points of 1, 2, 5, and 8 h were sampled to observe the changing trend. The untreated group was used as the control (no chemical treatment). Each graph shows the mean and SD of three independent experiments. */**, different from control as revealed by t-test, p < 0.0.5/p < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499716&req=5

Figure 5: Expression analyses ofAhHDA1and stress resistance genes following PEG treatment by qRT-PCR. Time points of 1, 2, 5, and 8 h were sampled to observe the changing trend. The untreated group was used as the control (no chemical treatment). Each graph shows the mean and SD of three independent experiments. */**, different from control as revealed by t-test, p < 0.0.5/p < 0.01.
Mentions: The effect of osmotic stress and exogenous ABA function on AhHDA1 was examined and it was found to be up-regulated by both PEG and ABA early in the response to both treatments (Figures 4, 5). However, the mechanisms underlying these responses are not known, and therefore it is not clear whether ABA and osmotic stress act on AhHDA1 via a common pathway or via independent pathways. Given that AhHDA1 transcription begins to increase significantly after 1 h of ABA treatment, but not until 2 h after PEG treatment, and that H3K14ac increases from 1 h of ABA treatment, while H3K9ac increases from 2 h of PEG treatment, it is possible that ABA-dependent stress-responsive genes are activated through modification of the H3K14 locus, and ABA-independent stress-responsive genes are activated at the H3K9 locus. The increased AhHDA1 expression induced by PEG or ABA might result from rapid changes in the HDAC and HAT “switches,” which re-balance histone and deacetylation.

Bottom Line: Using RNA-seq data for peanut, we found a RPD3/HDA1-like superfamily histone deacetylase (HDAC), termed AhHDA1, whose gene is up-regulated by PEG-induced water limitation and ABA signaling.To understand whether and how osmotic stress and ABA mediate the peanut stress response by epigenetics, the expression of AhHDA1 and stress-responsive genes following treatment with PEG, ABA, and the specific HDAC inhibitor trichostatin A (TSA) were analyzed.AhHDA1 transcript levels were enhanced by all three treatments, as was expression of peanut transcription factor genes, indicating that AhHDA1 might be involved in the epigenetic regulation of stress resistance genes that comprise the responses to osmotic stress and ABA.

View Article: PubMed Central - PubMed

Affiliation: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University Guangzhou, China.

ABSTRACT
Histone acetylation, which together with histone methylation regulates gene activity in response to stress, is an important epigenetic modification. There is an increasing research focus on histone acetylation in crops, but there is no information to date in peanut (Arachis hypogaea). We showed that osmotic stress and ABA affect the acetylation of histone H3 loci in peanut seedlings by immunoblotting experiments. Using RNA-seq data for peanut, we found a RPD3/HDA1-like superfamily histone deacetylase (HDAC), termed AhHDA1, whose gene is up-regulated by PEG-induced water limitation and ABA signaling. We isolated and characterized AhHDA1 from A. hypogaea, showing that AhHDA1 is very similar to an Arabidopsis HDAC (AtHDA6) and, in recombinant form, possesses HDAC activity. To understand whether and how osmotic stress and ABA mediate the peanut stress response by epigenetics, the expression of AhHDA1 and stress-responsive genes following treatment with PEG, ABA, and the specific HDAC inhibitor trichostatin A (TSA) were analyzed. AhHDA1 transcript levels were enhanced by all three treatments, as was expression of peanut transcription factor genes, indicating that AhHDA1 might be involved in the epigenetic regulation of stress resistance genes that comprise the responses to osmotic stress and ABA.

No MeSH data available.