Limits...
Isolation and characterization of an osmotic stress and ABA induced histone deacetylase in Arachis hygogaea.

Su LC, Deng B, Liu S, Li LM, Hu B, Zhong YT, Li L - Front Plant Sci (2015)

Bottom Line: Using RNA-seq data for peanut, we found a RPD3/HDA1-like superfamily histone deacetylase (HDAC), termed AhHDA1, whose gene is up-regulated by PEG-induced water limitation and ABA signaling.To understand whether and how osmotic stress and ABA mediate the peanut stress response by epigenetics, the expression of AhHDA1 and stress-responsive genes following treatment with PEG, ABA, and the specific HDAC inhibitor trichostatin A (TSA) were analyzed.AhHDA1 transcript levels were enhanced by all three treatments, as was expression of peanut transcription factor genes, indicating that AhHDA1 might be involved in the epigenetic regulation of stress resistance genes that comprise the responses to osmotic stress and ABA.

View Article: PubMed Central - PubMed

Affiliation: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University Guangzhou, China.

ABSTRACT
Histone acetylation, which together with histone methylation regulates gene activity in response to stress, is an important epigenetic modification. There is an increasing research focus on histone acetylation in crops, but there is no information to date in peanut (Arachis hypogaea). We showed that osmotic stress and ABA affect the acetylation of histone H3 loci in peanut seedlings by immunoblotting experiments. Using RNA-seq data for peanut, we found a RPD3/HDA1-like superfamily histone deacetylase (HDAC), termed AhHDA1, whose gene is up-regulated by PEG-induced water limitation and ABA signaling. We isolated and characterized AhHDA1 from A. hypogaea, showing that AhHDA1 is very similar to an Arabidopsis HDAC (AtHDA6) and, in recombinant form, possesses HDAC activity. To understand whether and how osmotic stress and ABA mediate the peanut stress response by epigenetics, the expression of AhHDA1 and stress-responsive genes following treatment with PEG, ABA, and the specific HDAC inhibitor trichostatin A (TSA) were analyzed. AhHDA1 transcript levels were enhanced by all three treatments, as was expression of peanut transcription factor genes, indicating that AhHDA1 might be involved in the epigenetic regulation of stress resistance genes that comprise the responses to osmotic stress and ABA.

No MeSH data available.


Quantitative RT-PCR validations ofAhHDA1expression in different peanut tissues. Column chart showing expression of AhHDA1 in PL, plumule; RA, radicle; ME, mesocotyl; L, leaf; S, stem; R, root; and F, flower, respectively. Plumules, radicles, and mesocotyls were taken from peanut embroys which had been cultivated for 7 days germination. Leaves stems and roots were taken from four-leaf stage peanut seedlings. Flowers were taken from peanuts during budding. All plants were grown as started in Materials and Methods. All values ± Standard Error (SE) for n = 3 biological replicates. Each graph displays the mean and SD of three independent experiments. */**, different from control as revealed by t-test, p < 0.05/p < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4499716&req=5

Figure 3: Quantitative RT-PCR validations ofAhHDA1expression in different peanut tissues. Column chart showing expression of AhHDA1 in PL, plumule; RA, radicle; ME, mesocotyl; L, leaf; S, stem; R, root; and F, flower, respectively. Plumules, radicles, and mesocotyls were taken from peanut embroys which had been cultivated for 7 days germination. Leaves stems and roots were taken from four-leaf stage peanut seedlings. Flowers were taken from peanuts during budding. All plants were grown as started in Materials and Methods. All values ± Standard Error (SE) for n = 3 biological replicates. Each graph displays the mean and SD of three independent experiments. */**, different from control as revealed by t-test, p < 0.05/p < 0.01.

Mentions: Quantitative RT-PCR analysis was performed to examine the expression of AhHDA1 in untreated embryos (plumule, radicle, and mesocotyl) and four-leaf seedlings (leaf, stem, root, and flower) (Figure 3). AhHDA1 mRNA predominantly accumulates in radicle and mesocotyl of the embryo; similarly, in seedlings, AhHDA1 mRNA predominantly accumulates in stem and root.


Isolation and characterization of an osmotic stress and ABA induced histone deacetylase in Arachis hygogaea.

Su LC, Deng B, Liu S, Li LM, Hu B, Zhong YT, Li L - Front Plant Sci (2015)

Quantitative RT-PCR validations ofAhHDA1expression in different peanut tissues. Column chart showing expression of AhHDA1 in PL, plumule; RA, radicle; ME, mesocotyl; L, leaf; S, stem; R, root; and F, flower, respectively. Plumules, radicles, and mesocotyls were taken from peanut embroys which had been cultivated for 7 days germination. Leaves stems and roots were taken from four-leaf stage peanut seedlings. Flowers were taken from peanuts during budding. All plants were grown as started in Materials and Methods. All values ± Standard Error (SE) for n = 3 biological replicates. Each graph displays the mean and SD of three independent experiments. */**, different from control as revealed by t-test, p < 0.05/p < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499716&req=5

Figure 3: Quantitative RT-PCR validations ofAhHDA1expression in different peanut tissues. Column chart showing expression of AhHDA1 in PL, plumule; RA, radicle; ME, mesocotyl; L, leaf; S, stem; R, root; and F, flower, respectively. Plumules, radicles, and mesocotyls were taken from peanut embroys which had been cultivated for 7 days germination. Leaves stems and roots were taken from four-leaf stage peanut seedlings. Flowers were taken from peanuts during budding. All plants were grown as started in Materials and Methods. All values ± Standard Error (SE) for n = 3 biological replicates. Each graph displays the mean and SD of three independent experiments. */**, different from control as revealed by t-test, p < 0.05/p < 0.01.
Mentions: Quantitative RT-PCR analysis was performed to examine the expression of AhHDA1 in untreated embryos (plumule, radicle, and mesocotyl) and four-leaf seedlings (leaf, stem, root, and flower) (Figure 3). AhHDA1 mRNA predominantly accumulates in radicle and mesocotyl of the embryo; similarly, in seedlings, AhHDA1 mRNA predominantly accumulates in stem and root.

Bottom Line: Using RNA-seq data for peanut, we found a RPD3/HDA1-like superfamily histone deacetylase (HDAC), termed AhHDA1, whose gene is up-regulated by PEG-induced water limitation and ABA signaling.To understand whether and how osmotic stress and ABA mediate the peanut stress response by epigenetics, the expression of AhHDA1 and stress-responsive genes following treatment with PEG, ABA, and the specific HDAC inhibitor trichostatin A (TSA) were analyzed.AhHDA1 transcript levels were enhanced by all three treatments, as was expression of peanut transcription factor genes, indicating that AhHDA1 might be involved in the epigenetic regulation of stress resistance genes that comprise the responses to osmotic stress and ABA.

View Article: PubMed Central - PubMed

Affiliation: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University Guangzhou, China.

ABSTRACT
Histone acetylation, which together with histone methylation regulates gene activity in response to stress, is an important epigenetic modification. There is an increasing research focus on histone acetylation in crops, but there is no information to date in peanut (Arachis hypogaea). We showed that osmotic stress and ABA affect the acetylation of histone H3 loci in peanut seedlings by immunoblotting experiments. Using RNA-seq data for peanut, we found a RPD3/HDA1-like superfamily histone deacetylase (HDAC), termed AhHDA1, whose gene is up-regulated by PEG-induced water limitation and ABA signaling. We isolated and characterized AhHDA1 from A. hypogaea, showing that AhHDA1 is very similar to an Arabidopsis HDAC (AtHDA6) and, in recombinant form, possesses HDAC activity. To understand whether and how osmotic stress and ABA mediate the peanut stress response by epigenetics, the expression of AhHDA1 and stress-responsive genes following treatment with PEG, ABA, and the specific HDAC inhibitor trichostatin A (TSA) were analyzed. AhHDA1 transcript levels were enhanced by all three treatments, as was expression of peanut transcription factor genes, indicating that AhHDA1 might be involved in the epigenetic regulation of stress resistance genes that comprise the responses to osmotic stress and ABA.

No MeSH data available.