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Effect of Taekwondo Training on Physical Fitness and Growth Index According to IGF-1 Gene Polymorphism in Children.

Lee B, Kim K - Korean J. Physiol. Pharmacol. (2015)

Bottom Line: IGF-1 concentration significantly (p<0.05) increased in the heterozygote group, while HOMA-IR significantly (p<0.05) decreased in the homozygote group.Furthermore, there was a significant (p<0.05) decrease in glucose in both the homozygote and the non-carriers groups.The difference between physical fitness and growth index depending on the IGF-1 gene polymorphism after Taekwondo training did not show consistent impact.

View Article: PubMed Central - PubMed

Affiliation: Department of Physical Education, Keimyung University, Daegu 704-701, Korea.

ABSTRACT
This study analyzed the effect of regular Taekwondo training for 16 weeks on physical fitness and growth index depending on different IGF-1 gene polymorphisms. The subjects of the study were 44 male students who were 8 year years old. The IGF-1 gene showed the highest frequency of 18 CA repeat (190 bp) in 50% of subjects, and was found in the homozygote (n=11), heterozygote (n=22) and non-carriers (n=11). The results of the physical fitness and growth index among the gene polymorphism groups indicated no significant differences but the expected height of the non-carrier group was significantly high (p<0.05). After Taekwondo training, the homozygote group and the non-carrier groups demonstrated significant (p<0.05) increase in grip strength and in time in the standing with one leg while closing eyes test, respectively. Only the homozygote group had a significant (p< 0.05) increase in thigh circumference. IGF-1 concentration significantly (p<0.05) increased in the heterozygote group, while HOMA-IR significantly (p<0.05) decreased in the homozygote group. Furthermore, there was a significant (p<0.05) decrease in glucose in both the homozygote and the non-carriers groups. The difference between physical fitness and growth index depending on the IGF-1 gene polymorphism after Taekwondo training did not show consistent impact.

No MeSH data available.


The capillary electrophoresis of CA repetition Upper: CA repetitive heterogeneous; Middle: 4 CA repetitive heterogeneous; Lower: Homogeneous.
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Figure 1: The capillary electrophoresis of CA repetition Upper: CA repetitive heterogeneous; Middle: 4 CA repetitive heterogeneous; Lower: Homogeneous.

Mentions: 5 ml of the peripheral blood was taken from the subjects for genetic analysis and the separated genomic DNA was melted by 10 mMTris (Base) of the TE buffer solution and 1 mM EDTA (Disodium, Dihydrate) with the pH between 7.0 and 7.4, measured by the spectrophotometer and stored at -20℃. The IGF-1 gene analysis followed the method of Larsen et al. [7] and the polymerase chain reaction (PCR) was implemented to amplify the confirmed DNA, showing that the forward primer was FAM-5'-GCTAGCCAGCTGGTGTTATT-3' and the backward primer was 5'-ACCACTCTGGGAGAAGGTA-3'. The PCR was 1.5 mM MgCl2, 200 µM dNTP, for each 0.4 µmM forward and backward primer, platinum Taq DNA polymerase (Invitrogen, Carlsbad, CA, USA) 1.0 unit with the total amount of 25 µl. The PCR consists of 22 reaction processes of pre-denaturation for 5 minutes at 94℃, 20 seconds at 94℃, 20 seconds at 65℃ and 15 seconds at 72℃ and then the extended reaction for 5 minutes at 72℃. 0.5 µl of the amplified product, 10 ul of the Hi-Di Formamide and 0.2 µl of the Mapmarker 1000 size standard (Bioventures, USA) were mixed, denaturized for 5 minutes at 96℃ and left in the ice for 5 minutes. The capillary electrophoresis was performed with the Applied Biosystems 3100 DNA analyzer and the CA repeat of the samples was analyzed by GeneScan Analysis Software 3.7 and Genotyper Software 3.7 (Fig. 1).


Effect of Taekwondo Training on Physical Fitness and Growth Index According to IGF-1 Gene Polymorphism in Children.

Lee B, Kim K - Korean J. Physiol. Pharmacol. (2015)

The capillary electrophoresis of CA repetition Upper: CA repetitive heterogeneous; Middle: 4 CA repetitive heterogeneous; Lower: Homogeneous.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499646&req=5

Figure 1: The capillary electrophoresis of CA repetition Upper: CA repetitive heterogeneous; Middle: 4 CA repetitive heterogeneous; Lower: Homogeneous.
Mentions: 5 ml of the peripheral blood was taken from the subjects for genetic analysis and the separated genomic DNA was melted by 10 mMTris (Base) of the TE buffer solution and 1 mM EDTA (Disodium, Dihydrate) with the pH between 7.0 and 7.4, measured by the spectrophotometer and stored at -20℃. The IGF-1 gene analysis followed the method of Larsen et al. [7] and the polymerase chain reaction (PCR) was implemented to amplify the confirmed DNA, showing that the forward primer was FAM-5'-GCTAGCCAGCTGGTGTTATT-3' and the backward primer was 5'-ACCACTCTGGGAGAAGGTA-3'. The PCR was 1.5 mM MgCl2, 200 µM dNTP, for each 0.4 µmM forward and backward primer, platinum Taq DNA polymerase (Invitrogen, Carlsbad, CA, USA) 1.0 unit with the total amount of 25 µl. The PCR consists of 22 reaction processes of pre-denaturation for 5 minutes at 94℃, 20 seconds at 94℃, 20 seconds at 65℃ and 15 seconds at 72℃ and then the extended reaction for 5 minutes at 72℃. 0.5 µl of the amplified product, 10 ul of the Hi-Di Formamide and 0.2 µl of the Mapmarker 1000 size standard (Bioventures, USA) were mixed, denaturized for 5 minutes at 96℃ and left in the ice for 5 minutes. The capillary electrophoresis was performed with the Applied Biosystems 3100 DNA analyzer and the CA repeat of the samples was analyzed by GeneScan Analysis Software 3.7 and Genotyper Software 3.7 (Fig. 1).

Bottom Line: IGF-1 concentration significantly (p<0.05) increased in the heterozygote group, while HOMA-IR significantly (p<0.05) decreased in the homozygote group.Furthermore, there was a significant (p<0.05) decrease in glucose in both the homozygote and the non-carriers groups.The difference between physical fitness and growth index depending on the IGF-1 gene polymorphism after Taekwondo training did not show consistent impact.

View Article: PubMed Central - PubMed

Affiliation: Department of Physical Education, Keimyung University, Daegu 704-701, Korea.

ABSTRACT
This study analyzed the effect of regular Taekwondo training for 16 weeks on physical fitness and growth index depending on different IGF-1 gene polymorphisms. The subjects of the study were 44 male students who were 8 year years old. The IGF-1 gene showed the highest frequency of 18 CA repeat (190 bp) in 50% of subjects, and was found in the homozygote (n=11), heterozygote (n=22) and non-carriers (n=11). The results of the physical fitness and growth index among the gene polymorphism groups indicated no significant differences but the expected height of the non-carrier group was significantly high (p<0.05). After Taekwondo training, the homozygote group and the non-carrier groups demonstrated significant (p<0.05) increase in grip strength and in time in the standing with one leg while closing eyes test, respectively. Only the homozygote group had a significant (p< 0.05) increase in thigh circumference. IGF-1 concentration significantly (p<0.05) increased in the heterozygote group, while HOMA-IR significantly (p<0.05) decreased in the homozygote group. Furthermore, there was a significant (p<0.05) decrease in glucose in both the homozygote and the non-carriers groups. The difference between physical fitness and growth index depending on the IGF-1 gene polymorphism after Taekwondo training did not show consistent impact.

No MeSH data available.