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Effects of Local Pancreatic Renin-Angiotensin System on the Microcirculation of Rat with Severe Acute Pancreatitis.

Pan Z, Feng L, Long H, Wang H, Feng J, Chen F - Korean J. Physiol. Pharmacol. (2015)

Bottom Line: Studies have found that local pancreatic renin-angiotensin system (RAS) was significantly upregulated in drug-induced SAP.The present study aimed to investigate the effects of angiotensin II receptors inhibitor valsartan on dual role of RAS in SAP in a rat model and to elucidate the underlying mechanisms. 3.8% sodium taurocholate (1 ml/kg) was injected to the pancreatic capsule in order for pancreatitis induction.The results suggest that pancreatic RAS plays a critical role in the regulation of pancreatic functions and demonstrates application potential as AT1 receptor antagonists.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology Surgery, The Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science & Technology, Wuhan 430014, Hubei, China.

ABSTRACT
Severe acute pancreatitis (SAP) is normally related to multiorgan dysfunction and local complications. Studies have found that local pancreatic renin-angiotensin system (RAS) was significantly upregulated in drug-induced SAP. The present study aimed to investigate the effects of angiotensin II receptors inhibitor valsartan on dual role of RAS in SAP in a rat model and to elucidate the underlying mechanisms. 3.8% sodium taurocholate (1 ml/kg) was injected to the pancreatic capsule in order for pancreatitis induction. Rats in the sham group were injected with normal saline in identical locations. We also investigated the regulation of experimentally induced SAP on local RAS expression in the pancreas through determination of the activities of serum amylase, lipase and myeloperoxidase, histological and biochemical analysis, radioimmunoassay, fluorescence quantitative PCR and Western blot analysis. The results indicated that valsartan could effectively suppress the local RAS to protect against experimental acute pancreatitis through inhibition of microcirculation disturbances and inflammation. The results suggest that pancreatic RAS plays a critical role in the regulation of pancreatic functions and demonstrates application potential as AT1 receptor antagonists. Moreover, other RAS inhibitors could be a new therapeutic target in acute pancreatitis.

No MeSH data available.


Related in: MedlinePlus

P-selectin expression on platelets in three experimental groups by using flow cytometric analysis. The values represent the percentages of P-selectin positive cells.
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Figure 3: P-selectin expression on platelets in three experimental groups by using flow cytometric analysis. The values represent the percentages of P-selectin positive cells.

Mentions: In the present study, P-selectin expression in platelets in different groups by using flow cytometry. As shown in Fig. 3, in the SO, SAP and SAP+V groups, the level of P-seletin were 15%, 34%, and 23% respectively. The level of P-selectin was significantly higher in SAP and SAP+V groups (p<0.05), according to the results of SO, SAP, and SAP+V groups. However, valsartan treatment significantly reduced the P-selectin level in SAP-induced rats (p<0.05).


Effects of Local Pancreatic Renin-Angiotensin System on the Microcirculation of Rat with Severe Acute Pancreatitis.

Pan Z, Feng L, Long H, Wang H, Feng J, Chen F - Korean J. Physiol. Pharmacol. (2015)

P-selectin expression on platelets in three experimental groups by using flow cytometric analysis. The values represent the percentages of P-selectin positive cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499641&req=5

Figure 3: P-selectin expression on platelets in three experimental groups by using flow cytometric analysis. The values represent the percentages of P-selectin positive cells.
Mentions: In the present study, P-selectin expression in platelets in different groups by using flow cytometry. As shown in Fig. 3, in the SO, SAP and SAP+V groups, the level of P-seletin were 15%, 34%, and 23% respectively. The level of P-selectin was significantly higher in SAP and SAP+V groups (p<0.05), according to the results of SO, SAP, and SAP+V groups. However, valsartan treatment significantly reduced the P-selectin level in SAP-induced rats (p<0.05).

Bottom Line: Studies have found that local pancreatic renin-angiotensin system (RAS) was significantly upregulated in drug-induced SAP.The present study aimed to investigate the effects of angiotensin II receptors inhibitor valsartan on dual role of RAS in SAP in a rat model and to elucidate the underlying mechanisms. 3.8% sodium taurocholate (1 ml/kg) was injected to the pancreatic capsule in order for pancreatitis induction.The results suggest that pancreatic RAS plays a critical role in the regulation of pancreatic functions and demonstrates application potential as AT1 receptor antagonists.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology Surgery, The Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science & Technology, Wuhan 430014, Hubei, China.

ABSTRACT
Severe acute pancreatitis (SAP) is normally related to multiorgan dysfunction and local complications. Studies have found that local pancreatic renin-angiotensin system (RAS) was significantly upregulated in drug-induced SAP. The present study aimed to investigate the effects of angiotensin II receptors inhibitor valsartan on dual role of RAS in SAP in a rat model and to elucidate the underlying mechanisms. 3.8% sodium taurocholate (1 ml/kg) was injected to the pancreatic capsule in order for pancreatitis induction. Rats in the sham group were injected with normal saline in identical locations. We also investigated the regulation of experimentally induced SAP on local RAS expression in the pancreas through determination of the activities of serum amylase, lipase and myeloperoxidase, histological and biochemical analysis, radioimmunoassay, fluorescence quantitative PCR and Western blot analysis. The results indicated that valsartan could effectively suppress the local RAS to protect against experimental acute pancreatitis through inhibition of microcirculation disturbances and inflammation. The results suggest that pancreatic RAS plays a critical role in the regulation of pancreatic functions and demonstrates application potential as AT1 receptor antagonists. Moreover, other RAS inhibitors could be a new therapeutic target in acute pancreatitis.

No MeSH data available.


Related in: MedlinePlus