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Effects of Local Pancreatic Renin-Angiotensin System on the Microcirculation of Rat with Severe Acute Pancreatitis.

Pan Z, Feng L, Long H, Wang H, Feng J, Chen F - Korean J. Physiol. Pharmacol. (2015)

Bottom Line: Studies have found that local pancreatic renin-angiotensin system (RAS) was significantly upregulated in drug-induced SAP.The present study aimed to investigate the effects of angiotensin II receptors inhibitor valsartan on dual role of RAS in SAP in a rat model and to elucidate the underlying mechanisms. 3.8% sodium taurocholate (1 ml/kg) was injected to the pancreatic capsule in order for pancreatitis induction.The results suggest that pancreatic RAS plays a critical role in the regulation of pancreatic functions and demonstrates application potential as AT1 receptor antagonists.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology Surgery, The Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science & Technology, Wuhan 430014, Hubei, China.

ABSTRACT
Severe acute pancreatitis (SAP) is normally related to multiorgan dysfunction and local complications. Studies have found that local pancreatic renin-angiotensin system (RAS) was significantly upregulated in drug-induced SAP. The present study aimed to investigate the effects of angiotensin II receptors inhibitor valsartan on dual role of RAS in SAP in a rat model and to elucidate the underlying mechanisms. 3.8% sodium taurocholate (1 ml/kg) was injected to the pancreatic capsule in order for pancreatitis induction. Rats in the sham group were injected with normal saline in identical locations. We also investigated the regulation of experimentally induced SAP on local RAS expression in the pancreas through determination of the activities of serum amylase, lipase and myeloperoxidase, histological and biochemical analysis, radioimmunoassay, fluorescence quantitative PCR and Western blot analysis. The results indicated that valsartan could effectively suppress the local RAS to protect against experimental acute pancreatitis through inhibition of microcirculation disturbances and inflammation. The results suggest that pancreatic RAS plays a critical role in the regulation of pancreatic functions and demonstrates application potential as AT1 receptor antagonists. Moreover, other RAS inhibitors could be a new therapeutic target in acute pancreatitis.

No MeSH data available.


Related in: MedlinePlus

Western blot analysis of the expression levels of Ang II, AT1 receptor and ICAM-1. Each bar represents the mean±SD from three samples (*p<0.05 vs. control).
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Figure 2: Western blot analysis of the expression levels of Ang II, AT1 receptor and ICAM-1. Each bar represents the mean±SD from three samples (*p<0.05 vs. control).

Mentions: The protein levels of Ang II, AT1 receptor and ICAM-1 were detected through Western blot analysis. As shown in Fig. 2, the protein level of β-actin indicated that the amount of protein in each band was equal. According to the results of the analysis, protein levels of Ang II, AT1 receptor and ICAM-1 significantly increased in SAP and SAP+V groups compared with SO group. In contrast, valsartan treatment caused a significant increase in the expression levels of Ang II, AT1 receptor and ICAM-1 in SAP+V groups when compared to SAP group.


Effects of Local Pancreatic Renin-Angiotensin System on the Microcirculation of Rat with Severe Acute Pancreatitis.

Pan Z, Feng L, Long H, Wang H, Feng J, Chen F - Korean J. Physiol. Pharmacol. (2015)

Western blot analysis of the expression levels of Ang II, AT1 receptor and ICAM-1. Each bar represents the mean±SD from three samples (*p<0.05 vs. control).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4499641&req=5

Figure 2: Western blot analysis of the expression levels of Ang II, AT1 receptor and ICAM-1. Each bar represents the mean±SD from three samples (*p<0.05 vs. control).
Mentions: The protein levels of Ang II, AT1 receptor and ICAM-1 were detected through Western blot analysis. As shown in Fig. 2, the protein level of β-actin indicated that the amount of protein in each band was equal. According to the results of the analysis, protein levels of Ang II, AT1 receptor and ICAM-1 significantly increased in SAP and SAP+V groups compared with SO group. In contrast, valsartan treatment caused a significant increase in the expression levels of Ang II, AT1 receptor and ICAM-1 in SAP+V groups when compared to SAP group.

Bottom Line: Studies have found that local pancreatic renin-angiotensin system (RAS) was significantly upregulated in drug-induced SAP.The present study aimed to investigate the effects of angiotensin II receptors inhibitor valsartan on dual role of RAS in SAP in a rat model and to elucidate the underlying mechanisms. 3.8% sodium taurocholate (1 ml/kg) was injected to the pancreatic capsule in order for pancreatitis induction.The results suggest that pancreatic RAS plays a critical role in the regulation of pancreatic functions and demonstrates application potential as AT1 receptor antagonists.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology Surgery, The Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science & Technology, Wuhan 430014, Hubei, China.

ABSTRACT
Severe acute pancreatitis (SAP) is normally related to multiorgan dysfunction and local complications. Studies have found that local pancreatic renin-angiotensin system (RAS) was significantly upregulated in drug-induced SAP. The present study aimed to investigate the effects of angiotensin II receptors inhibitor valsartan on dual role of RAS in SAP in a rat model and to elucidate the underlying mechanisms. 3.8% sodium taurocholate (1 ml/kg) was injected to the pancreatic capsule in order for pancreatitis induction. Rats in the sham group were injected with normal saline in identical locations. We also investigated the regulation of experimentally induced SAP on local RAS expression in the pancreas through determination of the activities of serum amylase, lipase and myeloperoxidase, histological and biochemical analysis, radioimmunoassay, fluorescence quantitative PCR and Western blot analysis. The results indicated that valsartan could effectively suppress the local RAS to protect against experimental acute pancreatitis through inhibition of microcirculation disturbances and inflammation. The results suggest that pancreatic RAS plays a critical role in the regulation of pancreatic functions and demonstrates application potential as AT1 receptor antagonists. Moreover, other RAS inhibitors could be a new therapeutic target in acute pancreatitis.

No MeSH data available.


Related in: MedlinePlus