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Angiogenic Potential of Human Neonatal Foreskin Stromal Cells in the Chick Embryo Chorioallantoic Membrane Model.

Vishnubalaji R, Atteya M, Al-Nbaheen M, Oreffo RO, Aldahmash A, Alajez NM - Stem Cells Int (2015)

Bottom Line: When hNSSCs were seeded onto the top of the CAM, human von Willebrand factor (hVWF), CD31, smooth muscle actin (SMA), and factor XIIIa positive cells were observed in the chick endothelium.Interestingly, undifferentiated hNSSCs showed a propensity to differentiate towards ectoderm with indication of epidermal formation with cells positive for CD1a, CK5/6, CK19, FXIIIa, and S-100 cells, which warrant further investigation.Our findings imply a potential angiogenic role for hNSSCs ex vivo in the differentiated and undifferentiated state, with potential contribution to blood vessel formation and potential application in tissue regeneration and vascularization.

View Article: PubMed Central - PubMed

Affiliation: Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh 11461, Saudi Arabia.

ABSTRACT
Several studies have demonstrated the multipotentiality of human neonatal foreskin stromal cells (hNSSCs) as being able to differentiate into adipocytes and osteoblasts and potentially other cell types. Recently, we demonstrated that hNSSCs play a role during in vitro angiogenesis and appear to possess a capacity to differentiate into endothelial-like cells; however, their angiogenic potential within an ex vivo environment remains unclear. Current study shows hNSSCs to display significant migration potential in the undifferentiated state and high responsiveness in the in vitro wound healing scratch assay. When hNSSCs were seeded onto the top of the CAM, human von Willebrand factor (hVWF), CD31, smooth muscle actin (SMA), and factor XIIIa positive cells were observed in the chick endothelium. CAMs transplanted with endothelial-differentiated hNSSCs displayed a higher number of blood vessels containing hNSSCs compared to CAMs transplanted with undifferentiated hNSSCs. Interestingly, undifferentiated hNSSCs showed a propensity to differentiate towards ectoderm with indication of epidermal formation with cells positive for CD1a, CK5/6, CK19, FXIIIa, and S-100 cells, which warrant further investigation. Our findings imply a potential angiogenic role for hNSSCs ex vivo in the differentiated and undifferentiated state, with potential contribution to blood vessel formation and potential application in tissue regeneration and vascularization.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical staining of CAM alone and CAM grafted hNSSCs for ectoderm-related markers. CAMs were grown as in Figure 4 and subsequently were processed for IHC. Brown colour indicates the positive area of respective skin lineage associated proteins, CD1a, CK 5/6, CK19, and S-100. CK: Cytokeratin (Bar = 100 μm).
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fig5: Immunohistochemical staining of CAM alone and CAM grafted hNSSCs for ectoderm-related markers. CAMs were grown as in Figure 4 and subsequently were processed for IHC. Brown colour indicates the positive area of respective skin lineage associated proteins, CD1a, CK 5/6, CK19, and S-100. CK: Cytokeratin (Bar = 100 μm).

Mentions: CAMs transplanted with undifferentiated hNSSCs exhibited modest staining for angiogenic markers and a modest number of neovessels (Figure 4, middle panel). Interestingly, large numbers of transplanted undifferentiated hNSSCs were observed in association with epithelial-like structures with irregular vasculature and an epidermal like phenotype that stained positively for CD1a, CK5/6, CK19, and S-100 (Figure 5, middle panel). The chick and CAM were observed to be negative for the human CD1a, CK5/6, CK19, and S-100 further suggesting that undifferentiated hNSSCs displayed epidermal potential. Studying the epidermal potential of the hNSSCs population in depth will be the subject of an independent investigation.


Angiogenic Potential of Human Neonatal Foreskin Stromal Cells in the Chick Embryo Chorioallantoic Membrane Model.

Vishnubalaji R, Atteya M, Al-Nbaheen M, Oreffo RO, Aldahmash A, Alajez NM - Stem Cells Int (2015)

Immunohistochemical staining of CAM alone and CAM grafted hNSSCs for ectoderm-related markers. CAMs were grown as in Figure 4 and subsequently were processed for IHC. Brown colour indicates the positive area of respective skin lineage associated proteins, CD1a, CK 5/6, CK19, and S-100. CK: Cytokeratin (Bar = 100 μm).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499640&req=5

fig5: Immunohistochemical staining of CAM alone and CAM grafted hNSSCs for ectoderm-related markers. CAMs were grown as in Figure 4 and subsequently were processed for IHC. Brown colour indicates the positive area of respective skin lineage associated proteins, CD1a, CK 5/6, CK19, and S-100. CK: Cytokeratin (Bar = 100 μm).
Mentions: CAMs transplanted with undifferentiated hNSSCs exhibited modest staining for angiogenic markers and a modest number of neovessels (Figure 4, middle panel). Interestingly, large numbers of transplanted undifferentiated hNSSCs were observed in association with epithelial-like structures with irregular vasculature and an epidermal like phenotype that stained positively for CD1a, CK5/6, CK19, and S-100 (Figure 5, middle panel). The chick and CAM were observed to be negative for the human CD1a, CK5/6, CK19, and S-100 further suggesting that undifferentiated hNSSCs displayed epidermal potential. Studying the epidermal potential of the hNSSCs population in depth will be the subject of an independent investigation.

Bottom Line: When hNSSCs were seeded onto the top of the CAM, human von Willebrand factor (hVWF), CD31, smooth muscle actin (SMA), and factor XIIIa positive cells were observed in the chick endothelium.Interestingly, undifferentiated hNSSCs showed a propensity to differentiate towards ectoderm with indication of epidermal formation with cells positive for CD1a, CK5/6, CK19, FXIIIa, and S-100 cells, which warrant further investigation.Our findings imply a potential angiogenic role for hNSSCs ex vivo in the differentiated and undifferentiated state, with potential contribution to blood vessel formation and potential application in tissue regeneration and vascularization.

View Article: PubMed Central - PubMed

Affiliation: Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh 11461, Saudi Arabia.

ABSTRACT
Several studies have demonstrated the multipotentiality of human neonatal foreskin stromal cells (hNSSCs) as being able to differentiate into adipocytes and osteoblasts and potentially other cell types. Recently, we demonstrated that hNSSCs play a role during in vitro angiogenesis and appear to possess a capacity to differentiate into endothelial-like cells; however, their angiogenic potential within an ex vivo environment remains unclear. Current study shows hNSSCs to display significant migration potential in the undifferentiated state and high responsiveness in the in vitro wound healing scratch assay. When hNSSCs were seeded onto the top of the CAM, human von Willebrand factor (hVWF), CD31, smooth muscle actin (SMA), and factor XIIIa positive cells were observed in the chick endothelium. CAMs transplanted with endothelial-differentiated hNSSCs displayed a higher number of blood vessels containing hNSSCs compared to CAMs transplanted with undifferentiated hNSSCs. Interestingly, undifferentiated hNSSCs showed a propensity to differentiate towards ectoderm with indication of epidermal formation with cells positive for CD1a, CK5/6, CK19, FXIIIa, and S-100 cells, which warrant further investigation. Our findings imply a potential angiogenic role for hNSSCs ex vivo in the differentiated and undifferentiated state, with potential contribution to blood vessel formation and potential application in tissue regeneration and vascularization.

No MeSH data available.


Related in: MedlinePlus