Limits...
Angiogenic Potential of Human Neonatal Foreskin Stromal Cells in the Chick Embryo Chorioallantoic Membrane Model.

Vishnubalaji R, Atteya M, Al-Nbaheen M, Oreffo RO, Aldahmash A, Alajez NM - Stem Cells Int (2015)

Bottom Line: When hNSSCs were seeded onto the top of the CAM, human von Willebrand factor (hVWF), CD31, smooth muscle actin (SMA), and factor XIIIa positive cells were observed in the chick endothelium.Interestingly, undifferentiated hNSSCs showed a propensity to differentiate towards ectoderm with indication of epidermal formation with cells positive for CD1a, CK5/6, CK19, FXIIIa, and S-100 cells, which warrant further investigation.Our findings imply a potential angiogenic role for hNSSCs ex vivo in the differentiated and undifferentiated state, with potential contribution to blood vessel formation and potential application in tissue regeneration and vascularization.

View Article: PubMed Central - PubMed

Affiliation: Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh 11461, Saudi Arabia.

ABSTRACT
Several studies have demonstrated the multipotentiality of human neonatal foreskin stromal cells (hNSSCs) as being able to differentiate into adipocytes and osteoblasts and potentially other cell types. Recently, we demonstrated that hNSSCs play a role during in vitro angiogenesis and appear to possess a capacity to differentiate into endothelial-like cells; however, their angiogenic potential within an ex vivo environment remains unclear. Current study shows hNSSCs to display significant migration potential in the undifferentiated state and high responsiveness in the in vitro wound healing scratch assay. When hNSSCs were seeded onto the top of the CAM, human von Willebrand factor (hVWF), CD31, smooth muscle actin (SMA), and factor XIIIa positive cells were observed in the chick endothelium. CAMs transplanted with endothelial-differentiated hNSSCs displayed a higher number of blood vessels containing hNSSCs compared to CAMs transplanted with undifferentiated hNSSCs. Interestingly, undifferentiated hNSSCs showed a propensity to differentiate towards ectoderm with indication of epidermal formation with cells positive for CD1a, CK5/6, CK19, FXIIIa, and S-100 cells, which warrant further investigation. Our findings imply a potential angiogenic role for hNSSCs ex vivo in the differentiated and undifferentiated state, with potential contribution to blood vessel formation and potential application in tissue regeneration and vascularization.

No MeSH data available.


Related in: MedlinePlus

Undifferentiated and differentiated hNSSCs exhibit different morphological changes when cultured on different surfaces. Undifferentiated and differentiated hNSSCs were cultured on tissue culture-treated (a and b), or untreated (c and d) or on matrigel (e and f). Morphological changes were subsequently observed and imaged (Bar = 200 μm).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4499640&req=5

fig2: Undifferentiated and differentiated hNSSCs exhibit different morphological changes when cultured on different surfaces. Undifferentiated and differentiated hNSSCs were cultured on tissue culture-treated (a and b), or untreated (c and d) or on matrigel (e and f). Morphological changes were subsequently observed and imaged (Bar = 200 μm).

Mentions: Interestingly, undifferentiated and endothelial-differentiated hNSSCs displayed distinct morphology upon placement on treated and nontreated tissue culture plastic surface. Undifferentiated hNSSCs maintained their spindle shape when cultured on treated and nontreated tissue cultures surfaces (Figures 2(a) and 2(c)), while those cells exhibited modest tube formation potential when plated on matrigel (Figures 2(e)). On the other hand, endothelial-differentiated cells were observed to form matrix-rich tubular-like structure on tissue culture treated plastic surface (Figure 2(b)) and to form spheres when cultured on nontissue culture treated surface (Figure 2(d)). Most notably, differentiated hNSSCs formed tight tubular-like structure when plated on matrigel (Figure 2(f)).


Angiogenic Potential of Human Neonatal Foreskin Stromal Cells in the Chick Embryo Chorioallantoic Membrane Model.

Vishnubalaji R, Atteya M, Al-Nbaheen M, Oreffo RO, Aldahmash A, Alajez NM - Stem Cells Int (2015)

Undifferentiated and differentiated hNSSCs exhibit different morphological changes when cultured on different surfaces. Undifferentiated and differentiated hNSSCs were cultured on tissue culture-treated (a and b), or untreated (c and d) or on matrigel (e and f). Morphological changes were subsequently observed and imaged (Bar = 200 μm).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499640&req=5

fig2: Undifferentiated and differentiated hNSSCs exhibit different morphological changes when cultured on different surfaces. Undifferentiated and differentiated hNSSCs were cultured on tissue culture-treated (a and b), or untreated (c and d) or on matrigel (e and f). Morphological changes were subsequently observed and imaged (Bar = 200 μm).
Mentions: Interestingly, undifferentiated and endothelial-differentiated hNSSCs displayed distinct morphology upon placement on treated and nontreated tissue culture plastic surface. Undifferentiated hNSSCs maintained their spindle shape when cultured on treated and nontreated tissue cultures surfaces (Figures 2(a) and 2(c)), while those cells exhibited modest tube formation potential when plated on matrigel (Figures 2(e)). On the other hand, endothelial-differentiated cells were observed to form matrix-rich tubular-like structure on tissue culture treated plastic surface (Figure 2(b)) and to form spheres when cultured on nontissue culture treated surface (Figure 2(d)). Most notably, differentiated hNSSCs formed tight tubular-like structure when plated on matrigel (Figure 2(f)).

Bottom Line: When hNSSCs were seeded onto the top of the CAM, human von Willebrand factor (hVWF), CD31, smooth muscle actin (SMA), and factor XIIIa positive cells were observed in the chick endothelium.Interestingly, undifferentiated hNSSCs showed a propensity to differentiate towards ectoderm with indication of epidermal formation with cells positive for CD1a, CK5/6, CK19, FXIIIa, and S-100 cells, which warrant further investigation.Our findings imply a potential angiogenic role for hNSSCs ex vivo in the differentiated and undifferentiated state, with potential contribution to blood vessel formation and potential application in tissue regeneration and vascularization.

View Article: PubMed Central - PubMed

Affiliation: Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Riyadh 11461, Saudi Arabia.

ABSTRACT
Several studies have demonstrated the multipotentiality of human neonatal foreskin stromal cells (hNSSCs) as being able to differentiate into adipocytes and osteoblasts and potentially other cell types. Recently, we demonstrated that hNSSCs play a role during in vitro angiogenesis and appear to possess a capacity to differentiate into endothelial-like cells; however, their angiogenic potential within an ex vivo environment remains unclear. Current study shows hNSSCs to display significant migration potential in the undifferentiated state and high responsiveness in the in vitro wound healing scratch assay. When hNSSCs were seeded onto the top of the CAM, human von Willebrand factor (hVWF), CD31, smooth muscle actin (SMA), and factor XIIIa positive cells were observed in the chick endothelium. CAMs transplanted with endothelial-differentiated hNSSCs displayed a higher number of blood vessels containing hNSSCs compared to CAMs transplanted with undifferentiated hNSSCs. Interestingly, undifferentiated hNSSCs showed a propensity to differentiate towards ectoderm with indication of epidermal formation with cells positive for CD1a, CK5/6, CK19, FXIIIa, and S-100 cells, which warrant further investigation. Our findings imply a potential angiogenic role for hNSSCs ex vivo in the differentiated and undifferentiated state, with potential contribution to blood vessel formation and potential application in tissue regeneration and vascularization.

No MeSH data available.


Related in: MedlinePlus