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The Genetic Deletion of 6q21 and PRDM1 and Clinical Implications in Extranodal NK/T Cell Lymphoma, Nasal Type.

Liang L, Zhang Z, Wang Y, Nong L, Zheng Y, Qu L, Zhang B, Li T - Biomed Res Int (2015)

Bottom Line: However, genetic deletion of 6q21 and/or PRDM1 was not correlated with clinicopathological features of EN-NK/T-NT, while PRDM1 expression showed positive effect on the outcome of patients as those as disease site, B symptom, and clinical stage.Thus, heterozygous deletion of 6q21 and/or PRDM1 was frequently detected in EN-NK/T-NT and correlated with downregulation of PRDM1.But the prognostic role of genetic deletion needs to be further evaluated in larger cohort.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Peking University First Hospital, Beijing 100034, China.

ABSTRACT
6q21 genetic deletion has been frequently detected in extranodal NK/T cell lymphoma, nasal type (EN-NK/T-NT), and PRDM1 is considered as candidate gene. However, direct detection of PRDM1 deletion has not been well documented. We investigated genetic alterations of 6q21 and PRDM1 in 43 cases of EN-NK/T-NT and cell lines by FISH. PRDM1 expression was evaluated by immunohistochemistry and Western blot. The correlation between genetic alteration and PRDM1 expression and the significance in clinic-pathologic were analyzed. Heterozygous deletion of 6q21 and/or PRDM1 was observed in 24 of 43 cases (55.81%) of EN-NK/T-NT including 16 cases (37.21%) for 6q21 deletion and 19 cases (44.19%) for PRDM1 deletion. Similarly, heterozygous codeletion of 6q21 and PRDM1 was identified in NK92 and NKL cells. The heterozygous deletion of 6q21 and/or PRDM1 was correlated with PRDM1 expression. However, genetic deletion of 6q21 and/or PRDM1 was not correlated with clinicopathological features of EN-NK/T-NT, while PRDM1 expression showed positive effect on the outcome of patients as those as disease site, B symptom, and clinical stage. Thus, heterozygous deletion of 6q21 and/or PRDM1 was frequently detected in EN-NK/T-NT and correlated with downregulation of PRDM1. But the prognostic role of genetic deletion needs to be further evaluated in larger cohort.

No MeSH data available.


Related in: MedlinePlus

Kaplan-Meier survival analysis of PRDM1 immunostaining, 6q21 deletion, and PRDM1 deletion in follow-up cohort of 40 EN-NK/T-NT cases. PRDM1 positive immunostaining predicted a favorable effect on overall survival (OS) (a, P = 0.034) and failure-free survival (FFS) (b, P = 0.017) of EN-NK/T-NT patients. However, the status of 6q21 (c, d) and specific PRDM1 gene alone (e, f) showed no significant correlation with the OS and FFS as those of the deletion of 6q21 and/or specific PRDM1 gene (g, P = 0.469; h, P = 0.866).
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fig4: Kaplan-Meier survival analysis of PRDM1 immunostaining, 6q21 deletion, and PRDM1 deletion in follow-up cohort of 40 EN-NK/T-NT cases. PRDM1 positive immunostaining predicted a favorable effect on overall survival (OS) (a, P = 0.034) and failure-free survival (FFS) (b, P = 0.017) of EN-NK/T-NT patients. However, the status of 6q21 (c, d) and specific PRDM1 gene alone (e, f) showed no significant correlation with the OS and FFS as those of the deletion of 6q21 and/or specific PRDM1 gene (g, P = 0.469; h, P = 0.866).

Mentions: As shown in Table 3, 6q21 heterozygous deletion showed no significant association with sex, age, angiocentric infiltration, necrosis, site, EBER, B symptom, Ann arbor stage, and the outcome of patients (P > 0.05). Similar results were found between PRDM1 heterozygous deletion and these clinical factors (Table 3). In addition, 6q21 heterozygous deletion was not significantly correlated with the OS and FFS of patients (Figure 4). Similarly, the status of specific PRDM1 gene showed no significant correlation with the OS and FFS (Figure 4). The clinical significance of the genetic deletion with either 6q21 and/or PRDM1 was also analysed. No significant correlation was found among them (Table 3, Figure 4). Furthermore, the clinical significance of 11 cases with codeletion of 6q21 and PRDM1 also showed no clinical significance (data not shown).


The Genetic Deletion of 6q21 and PRDM1 and Clinical Implications in Extranodal NK/T Cell Lymphoma, Nasal Type.

Liang L, Zhang Z, Wang Y, Nong L, Zheng Y, Qu L, Zhang B, Li T - Biomed Res Int (2015)

Kaplan-Meier survival analysis of PRDM1 immunostaining, 6q21 deletion, and PRDM1 deletion in follow-up cohort of 40 EN-NK/T-NT cases. PRDM1 positive immunostaining predicted a favorable effect on overall survival (OS) (a, P = 0.034) and failure-free survival (FFS) (b, P = 0.017) of EN-NK/T-NT patients. However, the status of 6q21 (c, d) and specific PRDM1 gene alone (e, f) showed no significant correlation with the OS and FFS as those of the deletion of 6q21 and/or specific PRDM1 gene (g, P = 0.469; h, P = 0.866).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499638&req=5

fig4: Kaplan-Meier survival analysis of PRDM1 immunostaining, 6q21 deletion, and PRDM1 deletion in follow-up cohort of 40 EN-NK/T-NT cases. PRDM1 positive immunostaining predicted a favorable effect on overall survival (OS) (a, P = 0.034) and failure-free survival (FFS) (b, P = 0.017) of EN-NK/T-NT patients. However, the status of 6q21 (c, d) and specific PRDM1 gene alone (e, f) showed no significant correlation with the OS and FFS as those of the deletion of 6q21 and/or specific PRDM1 gene (g, P = 0.469; h, P = 0.866).
Mentions: As shown in Table 3, 6q21 heterozygous deletion showed no significant association with sex, age, angiocentric infiltration, necrosis, site, EBER, B symptom, Ann arbor stage, and the outcome of patients (P > 0.05). Similar results were found between PRDM1 heterozygous deletion and these clinical factors (Table 3). In addition, 6q21 heterozygous deletion was not significantly correlated with the OS and FFS of patients (Figure 4). Similarly, the status of specific PRDM1 gene showed no significant correlation with the OS and FFS (Figure 4). The clinical significance of the genetic deletion with either 6q21 and/or PRDM1 was also analysed. No significant correlation was found among them (Table 3, Figure 4). Furthermore, the clinical significance of 11 cases with codeletion of 6q21 and PRDM1 also showed no clinical significance (data not shown).

Bottom Line: However, genetic deletion of 6q21 and/or PRDM1 was not correlated with clinicopathological features of EN-NK/T-NT, while PRDM1 expression showed positive effect on the outcome of patients as those as disease site, B symptom, and clinical stage.Thus, heterozygous deletion of 6q21 and/or PRDM1 was frequently detected in EN-NK/T-NT and correlated with downregulation of PRDM1.But the prognostic role of genetic deletion needs to be further evaluated in larger cohort.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Peking University First Hospital, Beijing 100034, China.

ABSTRACT
6q21 genetic deletion has been frequently detected in extranodal NK/T cell lymphoma, nasal type (EN-NK/T-NT), and PRDM1 is considered as candidate gene. However, direct detection of PRDM1 deletion has not been well documented. We investigated genetic alterations of 6q21 and PRDM1 in 43 cases of EN-NK/T-NT and cell lines by FISH. PRDM1 expression was evaluated by immunohistochemistry and Western blot. The correlation between genetic alteration and PRDM1 expression and the significance in clinic-pathologic were analyzed. Heterozygous deletion of 6q21 and/or PRDM1 was observed in 24 of 43 cases (55.81%) of EN-NK/T-NT including 16 cases (37.21%) for 6q21 deletion and 19 cases (44.19%) for PRDM1 deletion. Similarly, heterozygous codeletion of 6q21 and PRDM1 was identified in NK92 and NKL cells. The heterozygous deletion of 6q21 and/or PRDM1 was correlated with PRDM1 expression. However, genetic deletion of 6q21 and/or PRDM1 was not correlated with clinicopathological features of EN-NK/T-NT, while PRDM1 expression showed positive effect on the outcome of patients as those as disease site, B symptom, and clinical stage. Thus, heterozygous deletion of 6q21 and/or PRDM1 was frequently detected in EN-NK/T-NT and correlated with downregulation of PRDM1. But the prognostic role of genetic deletion needs to be further evaluated in larger cohort.

No MeSH data available.


Related in: MedlinePlus