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The Genetic Deletion of 6q21 and PRDM1 and Clinical Implications in Extranodal NK/T Cell Lymphoma, Nasal Type.

Liang L, Zhang Z, Wang Y, Nong L, Zheng Y, Qu L, Zhang B, Li T - Biomed Res Int (2015)

Bottom Line: However, genetic deletion of 6q21 and/or PRDM1 was not correlated with clinicopathological features of EN-NK/T-NT, while PRDM1 expression showed positive effect on the outcome of patients as those as disease site, B symptom, and clinical stage.Thus, heterozygous deletion of 6q21 and/or PRDM1 was frequently detected in EN-NK/T-NT and correlated with downregulation of PRDM1.But the prognostic role of genetic deletion needs to be further evaluated in larger cohort.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Peking University First Hospital, Beijing 100034, China.

ABSTRACT
6q21 genetic deletion has been frequently detected in extranodal NK/T cell lymphoma, nasal type (EN-NK/T-NT), and PRDM1 is considered as candidate gene. However, direct detection of PRDM1 deletion has not been well documented. We investigated genetic alterations of 6q21 and PRDM1 in 43 cases of EN-NK/T-NT and cell lines by FISH. PRDM1 expression was evaluated by immunohistochemistry and Western blot. The correlation between genetic alteration and PRDM1 expression and the significance in clinic-pathologic were analyzed. Heterozygous deletion of 6q21 and/or PRDM1 was observed in 24 of 43 cases (55.81%) of EN-NK/T-NT including 16 cases (37.21%) for 6q21 deletion and 19 cases (44.19%) for PRDM1 deletion. Similarly, heterozygous codeletion of 6q21 and PRDM1 was identified in NK92 and NKL cells. The heterozygous deletion of 6q21 and/or PRDM1 was correlated with PRDM1 expression. However, genetic deletion of 6q21 and/or PRDM1 was not correlated with clinicopathological features of EN-NK/T-NT, while PRDM1 expression showed positive effect on the outcome of patients as those as disease site, B symptom, and clinical stage. Thus, heterozygous deletion of 6q21 and/or PRDM1 was frequently detected in EN-NK/T-NT and correlated with downregulation of PRDM1. But the prognostic role of genetic deletion needs to be further evaluated in larger cohort.

No MeSH data available.


Related in: MedlinePlus

Identification of 6q21 and PRDM1 gene deletion by dual-color FISH and PRDM1 protein expression by Western blot in cell lines. (a) Dual-color FISH analysis was performed in NK/T cell lymphoma cell lines (YT, NK92, and NKL) and control cell line (K562, human chronic myelogenous leukemia cell) as described in Figure 1. YT cells with strong PRDM1 protein expression showed no deletion of 6q21 and PRDM1 gene, while NK92 and NKL cells with weak PRDM1 expression displayed the heterozygous codeletion of 6q21 and PRDM1. In addition, control cell line K562 lack of PRDM1 expression showed no deletion of 6q21 or PRDM1. All are shown at 1000x magnification. (b) Representative picture of PRDM1 protein expression by Western blot in YT, NK92, NKL, and K562 cells. #No deletion of 6q21 or PRDM1 gene. △The heterozygous codeletion of 6q21 and PRDM1 gene.
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fig3: Identification of 6q21 and PRDM1 gene deletion by dual-color FISH and PRDM1 protein expression by Western blot in cell lines. (a) Dual-color FISH analysis was performed in NK/T cell lymphoma cell lines (YT, NK92, and NKL) and control cell line (K562, human chronic myelogenous leukemia cell) as described in Figure 1. YT cells with strong PRDM1 protein expression showed no deletion of 6q21 and PRDM1 gene, while NK92 and NKL cells with weak PRDM1 expression displayed the heterozygous codeletion of 6q21 and PRDM1. In addition, control cell line K562 lack of PRDM1 expression showed no deletion of 6q21 or PRDM1. All are shown at 1000x magnification. (b) Representative picture of PRDM1 protein expression by Western blot in YT, NK92, NKL, and K562 cells. #No deletion of 6q21 or PRDM1 gene. △The heterozygous codeletion of 6q21 and PRDM1 gene.

Mentions: In addition, three NK/T lymphoma cell lines were also subjected to FISH detection. The heterozygous codeletion of 6q21 and PRDM1 was found in NK92 and NKL cells, but no deletion was found in YT cells and control cell line K562 (Figure 3(a)).


The Genetic Deletion of 6q21 and PRDM1 and Clinical Implications in Extranodal NK/T Cell Lymphoma, Nasal Type.

Liang L, Zhang Z, Wang Y, Nong L, Zheng Y, Qu L, Zhang B, Li T - Biomed Res Int (2015)

Identification of 6q21 and PRDM1 gene deletion by dual-color FISH and PRDM1 protein expression by Western blot in cell lines. (a) Dual-color FISH analysis was performed in NK/T cell lymphoma cell lines (YT, NK92, and NKL) and control cell line (K562, human chronic myelogenous leukemia cell) as described in Figure 1. YT cells with strong PRDM1 protein expression showed no deletion of 6q21 and PRDM1 gene, while NK92 and NKL cells with weak PRDM1 expression displayed the heterozygous codeletion of 6q21 and PRDM1. In addition, control cell line K562 lack of PRDM1 expression showed no deletion of 6q21 or PRDM1. All are shown at 1000x magnification. (b) Representative picture of PRDM1 protein expression by Western blot in YT, NK92, NKL, and K562 cells. #No deletion of 6q21 or PRDM1 gene. △The heterozygous codeletion of 6q21 and PRDM1 gene.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499638&req=5

fig3: Identification of 6q21 and PRDM1 gene deletion by dual-color FISH and PRDM1 protein expression by Western blot in cell lines. (a) Dual-color FISH analysis was performed in NK/T cell lymphoma cell lines (YT, NK92, and NKL) and control cell line (K562, human chronic myelogenous leukemia cell) as described in Figure 1. YT cells with strong PRDM1 protein expression showed no deletion of 6q21 and PRDM1 gene, while NK92 and NKL cells with weak PRDM1 expression displayed the heterozygous codeletion of 6q21 and PRDM1. In addition, control cell line K562 lack of PRDM1 expression showed no deletion of 6q21 or PRDM1. All are shown at 1000x magnification. (b) Representative picture of PRDM1 protein expression by Western blot in YT, NK92, NKL, and K562 cells. #No deletion of 6q21 or PRDM1 gene. △The heterozygous codeletion of 6q21 and PRDM1 gene.
Mentions: In addition, three NK/T lymphoma cell lines were also subjected to FISH detection. The heterozygous codeletion of 6q21 and PRDM1 was found in NK92 and NKL cells, but no deletion was found in YT cells and control cell line K562 (Figure 3(a)).

Bottom Line: However, genetic deletion of 6q21 and/or PRDM1 was not correlated with clinicopathological features of EN-NK/T-NT, while PRDM1 expression showed positive effect on the outcome of patients as those as disease site, B symptom, and clinical stage.Thus, heterozygous deletion of 6q21 and/or PRDM1 was frequently detected in EN-NK/T-NT and correlated with downregulation of PRDM1.But the prognostic role of genetic deletion needs to be further evaluated in larger cohort.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Peking University First Hospital, Beijing 100034, China.

ABSTRACT
6q21 genetic deletion has been frequently detected in extranodal NK/T cell lymphoma, nasal type (EN-NK/T-NT), and PRDM1 is considered as candidate gene. However, direct detection of PRDM1 deletion has not been well documented. We investigated genetic alterations of 6q21 and PRDM1 in 43 cases of EN-NK/T-NT and cell lines by FISH. PRDM1 expression was evaluated by immunohistochemistry and Western blot. The correlation between genetic alteration and PRDM1 expression and the significance in clinic-pathologic were analyzed. Heterozygous deletion of 6q21 and/or PRDM1 was observed in 24 of 43 cases (55.81%) of EN-NK/T-NT including 16 cases (37.21%) for 6q21 deletion and 19 cases (44.19%) for PRDM1 deletion. Similarly, heterozygous codeletion of 6q21 and PRDM1 was identified in NK92 and NKL cells. The heterozygous deletion of 6q21 and/or PRDM1 was correlated with PRDM1 expression. However, genetic deletion of 6q21 and/or PRDM1 was not correlated with clinicopathological features of EN-NK/T-NT, while PRDM1 expression showed positive effect on the outcome of patients as those as disease site, B symptom, and clinical stage. Thus, heterozygous deletion of 6q21 and/or PRDM1 was frequently detected in EN-NK/T-NT and correlated with downregulation of PRDM1. But the prognostic role of genetic deletion needs to be further evaluated in larger cohort.

No MeSH data available.


Related in: MedlinePlus