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The Genetic Deletion of 6q21 and PRDM1 and Clinical Implications in Extranodal NK/T Cell Lymphoma, Nasal Type.

Liang L, Zhang Z, Wang Y, Nong L, Zheng Y, Qu L, Zhang B, Li T - Biomed Res Int (2015)

Bottom Line: However, genetic deletion of 6q21 and/or PRDM1 was not correlated with clinicopathological features of EN-NK/T-NT, while PRDM1 expression showed positive effect on the outcome of patients as those as disease site, B symptom, and clinical stage.Thus, heterozygous deletion of 6q21 and/or PRDM1 was frequently detected in EN-NK/T-NT and correlated with downregulation of PRDM1.But the prognostic role of genetic deletion needs to be further evaluated in larger cohort.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Peking University First Hospital, Beijing 100034, China.

ABSTRACT
6q21 genetic deletion has been frequently detected in extranodal NK/T cell lymphoma, nasal type (EN-NK/T-NT), and PRDM1 is considered as candidate gene. However, direct detection of PRDM1 deletion has not been well documented. We investigated genetic alterations of 6q21 and PRDM1 in 43 cases of EN-NK/T-NT and cell lines by FISH. PRDM1 expression was evaluated by immunohistochemistry and Western blot. The correlation between genetic alteration and PRDM1 expression and the significance in clinic-pathologic were analyzed. Heterozygous deletion of 6q21 and/or PRDM1 was observed in 24 of 43 cases (55.81%) of EN-NK/T-NT including 16 cases (37.21%) for 6q21 deletion and 19 cases (44.19%) for PRDM1 deletion. Similarly, heterozygous codeletion of 6q21 and PRDM1 was identified in NK92 and NKL cells. The heterozygous deletion of 6q21 and/or PRDM1 was correlated with PRDM1 expression. However, genetic deletion of 6q21 and/or PRDM1 was not correlated with clinicopathological features of EN-NK/T-NT, while PRDM1 expression showed positive effect on the outcome of patients as those as disease site, B symptom, and clinical stage. Thus, heterozygous deletion of 6q21 and/or PRDM1 was frequently detected in EN-NK/T-NT and correlated with downregulation of PRDM1. But the prognostic role of genetic deletion needs to be further evaluated in larger cohort.

No MeSH data available.


Related in: MedlinePlus

Two representative EN-NK/T-NT cases without deletion of 6q21 or PRDM1 gene. Dual-color FISH was performed with 6q21 probe or PRDM1 specific probe (labelled with 5-carboxyl-xrhodamine, red) and chromosome 6 centromere specific probe (labelled with 5-fluorescein, green), respectively. Case 3, (a), (b), and (c): one case without deletion of 6q21 (a, two green and two red) or PRDM1 gene (b, two green and two red) showed PRDM1 positive immunostaining (c). Case 4, (d), (e), and (f): another representative case without deletion of 6q21 (d, two green and two red) or PRDM1 gene (e, two green and two red) presented PRDM1 positive immunostaining (f). (a, b, d, and e at 1000x magnification; c and f at 400x magnification).
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fig2: Two representative EN-NK/T-NT cases without deletion of 6q21 or PRDM1 gene. Dual-color FISH was performed with 6q21 probe or PRDM1 specific probe (labelled with 5-carboxyl-xrhodamine, red) and chromosome 6 centromere specific probe (labelled with 5-fluorescein, green), respectively. Case 3, (a), (b), and (c): one case without deletion of 6q21 (a, two green and two red) or PRDM1 gene (b, two green and two red) showed PRDM1 positive immunostaining (c). Case 4, (d), (e), and (f): another representative case without deletion of 6q21 (d, two green and two red) or PRDM1 gene (e, two green and two red) presented PRDM1 positive immunostaining (f). (a, b, d, and e at 1000x magnification; c and f at 400x magnification).

Mentions: We found the significant positive correlation between 6q21 deletion and PRDM1 deletion by Spearman rank correlation coefficient test (P = 0.021, r = 0.381). Representative FISH images of the heterozygous deletion of 6q21 and PRDM1 gene in EN-NK/T-NT cases were displayed in Figures 1 and 2.


The Genetic Deletion of 6q21 and PRDM1 and Clinical Implications in Extranodal NK/T Cell Lymphoma, Nasal Type.

Liang L, Zhang Z, Wang Y, Nong L, Zheng Y, Qu L, Zhang B, Li T - Biomed Res Int (2015)

Two representative EN-NK/T-NT cases without deletion of 6q21 or PRDM1 gene. Dual-color FISH was performed with 6q21 probe or PRDM1 specific probe (labelled with 5-carboxyl-xrhodamine, red) and chromosome 6 centromere specific probe (labelled with 5-fluorescein, green), respectively. Case 3, (a), (b), and (c): one case without deletion of 6q21 (a, two green and two red) or PRDM1 gene (b, two green and two red) showed PRDM1 positive immunostaining (c). Case 4, (d), (e), and (f): another representative case without deletion of 6q21 (d, two green and two red) or PRDM1 gene (e, two green and two red) presented PRDM1 positive immunostaining (f). (a, b, d, and e at 1000x magnification; c and f at 400x magnification).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499638&req=5

fig2: Two representative EN-NK/T-NT cases without deletion of 6q21 or PRDM1 gene. Dual-color FISH was performed with 6q21 probe or PRDM1 specific probe (labelled with 5-carboxyl-xrhodamine, red) and chromosome 6 centromere specific probe (labelled with 5-fluorescein, green), respectively. Case 3, (a), (b), and (c): one case without deletion of 6q21 (a, two green and two red) or PRDM1 gene (b, two green and two red) showed PRDM1 positive immunostaining (c). Case 4, (d), (e), and (f): another representative case without deletion of 6q21 (d, two green and two red) or PRDM1 gene (e, two green and two red) presented PRDM1 positive immunostaining (f). (a, b, d, and e at 1000x magnification; c and f at 400x magnification).
Mentions: We found the significant positive correlation between 6q21 deletion and PRDM1 deletion by Spearman rank correlation coefficient test (P = 0.021, r = 0.381). Representative FISH images of the heterozygous deletion of 6q21 and PRDM1 gene in EN-NK/T-NT cases were displayed in Figures 1 and 2.

Bottom Line: However, genetic deletion of 6q21 and/or PRDM1 was not correlated with clinicopathological features of EN-NK/T-NT, while PRDM1 expression showed positive effect on the outcome of patients as those as disease site, B symptom, and clinical stage.Thus, heterozygous deletion of 6q21 and/or PRDM1 was frequently detected in EN-NK/T-NT and correlated with downregulation of PRDM1.But the prognostic role of genetic deletion needs to be further evaluated in larger cohort.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Peking University First Hospital, Beijing 100034, China.

ABSTRACT
6q21 genetic deletion has been frequently detected in extranodal NK/T cell lymphoma, nasal type (EN-NK/T-NT), and PRDM1 is considered as candidate gene. However, direct detection of PRDM1 deletion has not been well documented. We investigated genetic alterations of 6q21 and PRDM1 in 43 cases of EN-NK/T-NT and cell lines by FISH. PRDM1 expression was evaluated by immunohistochemistry and Western blot. The correlation between genetic alteration and PRDM1 expression and the significance in clinic-pathologic were analyzed. Heterozygous deletion of 6q21 and/or PRDM1 was observed in 24 of 43 cases (55.81%) of EN-NK/T-NT including 16 cases (37.21%) for 6q21 deletion and 19 cases (44.19%) for PRDM1 deletion. Similarly, heterozygous codeletion of 6q21 and PRDM1 was identified in NK92 and NKL cells. The heterozygous deletion of 6q21 and/or PRDM1 was correlated with PRDM1 expression. However, genetic deletion of 6q21 and/or PRDM1 was not correlated with clinicopathological features of EN-NK/T-NT, while PRDM1 expression showed positive effect on the outcome of patients as those as disease site, B symptom, and clinical stage. Thus, heterozygous deletion of 6q21 and/or PRDM1 was frequently detected in EN-NK/T-NT and correlated with downregulation of PRDM1. But the prognostic role of genetic deletion needs to be further evaluated in larger cohort.

No MeSH data available.


Related in: MedlinePlus