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Alendronate-Eluting Biphasic Calcium Phosphate (BCP) Scaffolds Stimulate Osteogenic Differentiation.

Kim SE, Yun YP, Lee DW, Kang EY, Jeong WJ, Lee B, Jeong MS, Kim HJ, Park K, Song HR - Biomed Res Int (2015)

Bottom Line: The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR).In vitro results revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone.Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, No. 80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.

ABSTRACT
Biphasic calcium phosphate (BCP) scaffolds have been widely used in orthopedic and dental fields as osteoconductive bone substitutes. However, BCP scaffolds are not satisfactory for the stimulation of osteogenic differentiation and maturation. To enhance osteogenic differentiation, we prepared alendronate- (ALN-) eluting BCP scaffolds. The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). An in vitro release study showed that release of ALN from ALN-eluting BCP scaffolds was sustained for up to 28 days. In vitro results revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone. Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.

No MeSH data available.


Related in: MedlinePlus

Real-time PCR analysis of mRNA gene expression of Runx2, alkaline phosphatase (ALP), osteocalcin (OCN), and osteopontin (OPN) of MG-63 cells grown on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP scaffolds after incubation for 7 and 21 days, respectively, (∗P < 0.05). The error bars represent mean ± SD (n = 5). These experiments were repeated three times.
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fig6: Real-time PCR analysis of mRNA gene expression of Runx2, alkaline phosphatase (ALP), osteocalcin (OCN), and osteopontin (OPN) of MG-63 cells grown on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP scaffolds after incubation for 7 and 21 days, respectively, (∗P < 0.05). The error bars represent mean ± SD (n = 5). These experiments were repeated three times.

Mentions: To further confirm osteogenic differentiation of MG-63 cells, expression of the osteogenic genes Runx2, ALP, OCN, and OPN in MG-63 cells cultured on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP scaffolds was evaluated by real-time PCR (Figure 6). At 7 and 21 days, Runx2, ALP, OCN, and OPN mRNA levels in MG-63 cells grown on ALN/BCP scaffolds were higher than those in cells grown on BCP scaffolds. In particular, Runx2 and ALP mRNA levels in MG-63 cells grown on ALN/BCP were significantly higher than those in cells grown on BCP scaffolds at 7 days (∗P < 0.05). However, there were no significant differences in Runx2 and ALP mRNA levels between BCP and ALN/BCP scaffolds at 21 days. On the other hand, there were no significant differences in OCN and OPN mRNA levels between BCP and ALN/BCP scaffolds at 7 days. At 21 days, however, OCN and OPN mRNA levels in cells grown on ALN/BCP scaffolds were markedly higher than those in cells grown on BCP scaffolds (∗P < 0.05).


Alendronate-Eluting Biphasic Calcium Phosphate (BCP) Scaffolds Stimulate Osteogenic Differentiation.

Kim SE, Yun YP, Lee DW, Kang EY, Jeong WJ, Lee B, Jeong MS, Kim HJ, Park K, Song HR - Biomed Res Int (2015)

Real-time PCR analysis of mRNA gene expression of Runx2, alkaline phosphatase (ALP), osteocalcin (OCN), and osteopontin (OPN) of MG-63 cells grown on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP scaffolds after incubation for 7 and 21 days, respectively, (∗P < 0.05). The error bars represent mean ± SD (n = 5). These experiments were repeated three times.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499637&req=5

fig6: Real-time PCR analysis of mRNA gene expression of Runx2, alkaline phosphatase (ALP), osteocalcin (OCN), and osteopontin (OPN) of MG-63 cells grown on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP scaffolds after incubation for 7 and 21 days, respectively, (∗P < 0.05). The error bars represent mean ± SD (n = 5). These experiments were repeated three times.
Mentions: To further confirm osteogenic differentiation of MG-63 cells, expression of the osteogenic genes Runx2, ALP, OCN, and OPN in MG-63 cells cultured on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP scaffolds was evaluated by real-time PCR (Figure 6). At 7 and 21 days, Runx2, ALP, OCN, and OPN mRNA levels in MG-63 cells grown on ALN/BCP scaffolds were higher than those in cells grown on BCP scaffolds. In particular, Runx2 and ALP mRNA levels in MG-63 cells grown on ALN/BCP were significantly higher than those in cells grown on BCP scaffolds at 7 days (∗P < 0.05). However, there were no significant differences in Runx2 and ALP mRNA levels between BCP and ALN/BCP scaffolds at 21 days. On the other hand, there were no significant differences in OCN and OPN mRNA levels between BCP and ALN/BCP scaffolds at 7 days. At 21 days, however, OCN and OPN mRNA levels in cells grown on ALN/BCP scaffolds were markedly higher than those in cells grown on BCP scaffolds (∗P < 0.05).

Bottom Line: The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR).In vitro results revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone.Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, No. 80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.

ABSTRACT
Biphasic calcium phosphate (BCP) scaffolds have been widely used in orthopedic and dental fields as osteoconductive bone substitutes. However, BCP scaffolds are not satisfactory for the stimulation of osteogenic differentiation and maturation. To enhance osteogenic differentiation, we prepared alendronate- (ALN-) eluting BCP scaffolds. The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). An in vitro release study showed that release of ALN from ALN-eluting BCP scaffolds was sustained for up to 28 days. In vitro results revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone. Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.

No MeSH data available.


Related in: MedlinePlus