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Alendronate-Eluting Biphasic Calcium Phosphate (BCP) Scaffolds Stimulate Osteogenic Differentiation.

Kim SE, Yun YP, Lee DW, Kang EY, Jeong WJ, Lee B, Jeong MS, Kim HJ, Park K, Song HR - Biomed Res Int (2015)

Bottom Line: The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR).In vitro results revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone.Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, No. 80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.

ABSTRACT
Biphasic calcium phosphate (BCP) scaffolds have been widely used in orthopedic and dental fields as osteoconductive bone substitutes. However, BCP scaffolds are not satisfactory for the stimulation of osteogenic differentiation and maturation. To enhance osteogenic differentiation, we prepared alendronate- (ALN-) eluting BCP scaffolds. The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). An in vitro release study showed that release of ALN from ALN-eluting BCP scaffolds was sustained for up to 28 days. In vitro results revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone. Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.

No MeSH data available.


Related in: MedlinePlus

(a) ALP activity of MG-63 cells cultured on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP scaffolds after culture for 3, 7, 10, and 14 days (∗P < 0.05) and (b) calcium deposition by MG-63 cells cultured on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP after culture for 21 days (∗P < 0.05). The error bars represent mean ± SD (n = 5). These experiments were repeated three times.
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fig5: (a) ALP activity of MG-63 cells cultured on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP scaffolds after culture for 3, 7, 10, and 14 days (∗P < 0.05) and (b) calcium deposition by MG-63 cells cultured on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP after culture for 21 days (∗P < 0.05). The error bars represent mean ± SD (n = 5). These experiments were repeated three times.

Mentions: Figure 5(a) shows the ALP activity of MG-63 cells cultured on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP scaffolds at 3, 7, 10, and 14 days. The ALP activity of MG-63 cells grown on all scaffolds gradually increased with incubation time up to 14 days. Also, as the coating amount of ALN on BCP scaffolds increased, the ALP activity of MG-63 cells also increased: ALP activity of ALN (1 mg)/BCP > ALN (0.1 mg)/BCP > BCP. Furthermore, the ALP activity of MG-63 cells cultured on ALN (1 mg)/BCP scaffolds was significantly higher than that of cells on ALN (0.1 mg)/BCP and BCP at all incubation time points (∗P < 0.05). The amount of calcium deposition was determined after culture of MG-63 cells for 21 days on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP scaffolds (Figure 5(b)). The amount of deposited calcium was 8.08 ± 0.18 for BCP, 11.81 ± 1.32 for ALN (0.1 mg)/BCP, and 13.24 ± 0.74 for ALN (1 mg)/BCP, respectively. Calcium deposition by MG-63 cells cultured on ALN/BCP scaffolds was significantly higher than that on BCP scaffolds (∗P < 0.05). However, the amount of calcium deposited by MG-63 cells cultured on the two ALN/BCP scaffolds was not significantly different after 21 days of culture.


Alendronate-Eluting Biphasic Calcium Phosphate (BCP) Scaffolds Stimulate Osteogenic Differentiation.

Kim SE, Yun YP, Lee DW, Kang EY, Jeong WJ, Lee B, Jeong MS, Kim HJ, Park K, Song HR - Biomed Res Int (2015)

(a) ALP activity of MG-63 cells cultured on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP scaffolds after culture for 3, 7, 10, and 14 days (∗P < 0.05) and (b) calcium deposition by MG-63 cells cultured on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP after culture for 21 days (∗P < 0.05). The error bars represent mean ± SD (n = 5). These experiments were repeated three times.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig5: (a) ALP activity of MG-63 cells cultured on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP scaffolds after culture for 3, 7, 10, and 14 days (∗P < 0.05) and (b) calcium deposition by MG-63 cells cultured on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP after culture for 21 days (∗P < 0.05). The error bars represent mean ± SD (n = 5). These experiments were repeated three times.
Mentions: Figure 5(a) shows the ALP activity of MG-63 cells cultured on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP scaffolds at 3, 7, 10, and 14 days. The ALP activity of MG-63 cells grown on all scaffolds gradually increased with incubation time up to 14 days. Also, as the coating amount of ALN on BCP scaffolds increased, the ALP activity of MG-63 cells also increased: ALP activity of ALN (1 mg)/BCP > ALN (0.1 mg)/BCP > BCP. Furthermore, the ALP activity of MG-63 cells cultured on ALN (1 mg)/BCP scaffolds was significantly higher than that of cells on ALN (0.1 mg)/BCP and BCP at all incubation time points (∗P < 0.05). The amount of calcium deposition was determined after culture of MG-63 cells for 21 days on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP scaffolds (Figure 5(b)). The amount of deposited calcium was 8.08 ± 0.18 for BCP, 11.81 ± 1.32 for ALN (0.1 mg)/BCP, and 13.24 ± 0.74 for ALN (1 mg)/BCP, respectively. Calcium deposition by MG-63 cells cultured on ALN/BCP scaffolds was significantly higher than that on BCP scaffolds (∗P < 0.05). However, the amount of calcium deposited by MG-63 cells cultured on the two ALN/BCP scaffolds was not significantly different after 21 days of culture.

Bottom Line: The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR).In vitro results revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone.Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, No. 80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.

ABSTRACT
Biphasic calcium phosphate (BCP) scaffolds have been widely used in orthopedic and dental fields as osteoconductive bone substitutes. However, BCP scaffolds are not satisfactory for the stimulation of osteogenic differentiation and maturation. To enhance osteogenic differentiation, we prepared alendronate- (ALN-) eluting BCP scaffolds. The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). An in vitro release study showed that release of ALN from ALN-eluting BCP scaffolds was sustained for up to 28 days. In vitro results revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone. Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.

No MeSH data available.


Related in: MedlinePlus