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Alendronate-Eluting Biphasic Calcium Phosphate (BCP) Scaffolds Stimulate Osteogenic Differentiation.

Kim SE, Yun YP, Lee DW, Kang EY, Jeong WJ, Lee B, Jeong MS, Kim HJ, Park K, Song HR - Biomed Res Int (2015)

Bottom Line: The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR).In vitro results revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone.Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, No. 80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.

ABSTRACT
Biphasic calcium phosphate (BCP) scaffolds have been widely used in orthopedic and dental fields as osteoconductive bone substitutes. However, BCP scaffolds are not satisfactory for the stimulation of osteogenic differentiation and maturation. To enhance osteogenic differentiation, we prepared alendronate- (ALN-) eluting BCP scaffolds. The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). An in vitro release study showed that release of ALN from ALN-eluting BCP scaffolds was sustained for up to 28 days. In vitro results revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone. Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.

No MeSH data available.


(a) SEM images of MG-63 cells cultured on (A) BCP, (B) ALN (0.1 mg)/BCP, and (C) ALN (1 mg)/BCP scaffolds. (b) F-actin staining of MG-63 cells cultured on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP after 24 hours of incubation. Bar: 80 μm. These experiments were repeated three times.
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fig4: (a) SEM images of MG-63 cells cultured on (A) BCP, (B) ALN (0.1 mg)/BCP, and (C) ALN (1 mg)/BCP scaffolds. (b) F-actin staining of MG-63 cells cultured on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP after 24 hours of incubation. Bar: 80 μm. These experiments were repeated three times.

Mentions: Figure 4 shows the cell morphology of MG-63 cells cultured on BCP and ALN/BCP scaffolds. As shown in Figure 4(a), the MG-63 cells adhered on the surface of BCP and ALN/BCP scaffolds after 24 hours of incubation. The adhered cells were spread on the surface of the scaffolds, and the number of adhered cells increased with an increase in the amount of ALN coating the BCP scaffolds (Figure 4(b)).


Alendronate-Eluting Biphasic Calcium Phosphate (BCP) Scaffolds Stimulate Osteogenic Differentiation.

Kim SE, Yun YP, Lee DW, Kang EY, Jeong WJ, Lee B, Jeong MS, Kim HJ, Park K, Song HR - Biomed Res Int (2015)

(a) SEM images of MG-63 cells cultured on (A) BCP, (B) ALN (0.1 mg)/BCP, and (C) ALN (1 mg)/BCP scaffolds. (b) F-actin staining of MG-63 cells cultured on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP after 24 hours of incubation. Bar: 80 μm. These experiments were repeated three times.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4499637&req=5

fig4: (a) SEM images of MG-63 cells cultured on (A) BCP, (B) ALN (0.1 mg)/BCP, and (C) ALN (1 mg)/BCP scaffolds. (b) F-actin staining of MG-63 cells cultured on BCP, ALN (0.1 mg)/BCP, and ALN (1 mg)/BCP after 24 hours of incubation. Bar: 80 μm. These experiments were repeated three times.
Mentions: Figure 4 shows the cell morphology of MG-63 cells cultured on BCP and ALN/BCP scaffolds. As shown in Figure 4(a), the MG-63 cells adhered on the surface of BCP and ALN/BCP scaffolds after 24 hours of incubation. The adhered cells were spread on the surface of the scaffolds, and the number of adhered cells increased with an increase in the amount of ALN coating the BCP scaffolds (Figure 4(b)).

Bottom Line: The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR).In vitro results revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone.Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery and Rare Diseases Institute, Korea University Medical College, Guro Hospital, No. 80, Guro-dong, Guro-gu, Seoul 152-703, Republic of Korea.

ABSTRACT
Biphasic calcium phosphate (BCP) scaffolds have been widely used in orthopedic and dental fields as osteoconductive bone substitutes. However, BCP scaffolds are not satisfactory for the stimulation of osteogenic differentiation and maturation. To enhance osteogenic differentiation, we prepared alendronate- (ALN-) eluting BCP scaffolds. The coating of ALN on BCP scaffolds was confirmed by scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDS), and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR). An in vitro release study showed that release of ALN from ALN-eluting BCP scaffolds was sustained for up to 28 days. In vitro results revealed that MG-63 cells grown on ALN-eluting BCP scaffolds exhibited increased ALP activity and calcium deposition and upregulated gene expression of Runx2, ALP, OCN, and OPN compared with the BCP scaffold alone. Therefore, this study suggests that ALN-eluting BCP scaffolds have the potential to effectively stimulate osteogenic differentiation.

No MeSH data available.