Limits...
Protective Effects of the Mushroom Lactarius deterrimus Extract on Systemic Oxidative Stress and Pancreatic Islets in Streptozotocin-Induced Diabetic Rats.

Mihailović M, Arambašić Јovanović J, Uskoković A, Grdović N, Dinić S, Vidović S, Poznanović G, Mujić I, Vidaković M - J Diabetes Res (2015)

Bottom Line: In addition to a systemic antioxidant effect, the administration of the extract to diabetic rats also had a positive localized effect on pancreatic islets where it decreased AGE formation, and increased the expression of chemokine CXCL12 protein that mediates the restoration of β-cell population through the activation of the serine/threonine-specific Akt protein kinase prosurvival pathway.As a result, the numbers of proliferating cell nuclear antigen- (PCNA-) and insulin-positive β-cells were increased.These results show that the ability of the L. deterrimus extract to alleviate oxidative stress and increase β-cell mass represents a therapeutic potential for diabetes management.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.

ABSTRACT
The aim of this study was to assess the in vivo effects of the extract of the medicinal mushroom, Lactarius deterrimus, when administered (60 mg/kg, i.p.) daily for four weeks to streptozotocin- (STZ-) induced diabetic rats. Diabetic rats treated with the L. deterrimus extract displayed several improved biochemical parameters in the circulation: reduced hyperglycemia, lower triglyceride concentration and reduced glycated hemoglobin, glycated serum protein, and advanced glycation end product (AGE) levels. This treatment also adjusted the diabetes-induced redox imbalance. Thus, higher activities of the antioxidative enzymes, superoxide dismutase, and catalase in the circulation were accompanied by increased levels of free intracellular thiols and glutathionylated proteins after treatment with the L. deterrimus extract. In addition to a systemic antioxidant effect, the administration of the extract to diabetic rats also had a positive localized effect on pancreatic islets where it decreased AGE formation, and increased the expression of chemokine CXCL12 protein that mediates the restoration of β-cell population through the activation of the serine/threonine-specific Akt protein kinase prosurvival pathway. As a result, the numbers of proliferating cell nuclear antigen- (PCNA-) and insulin-positive β-cells were increased. These results show that the ability of the L. deterrimus extract to alleviate oxidative stress and increase β-cell mass represents a therapeutic potential for diabetes management.

No MeSH data available.


Related in: MedlinePlus

The effect of L. deterrimus extract administration on the prosurvival pathway and β-cell proliferation in pancreatic islets. Light photomicrographs of immunohistochemical staining for pAkt and PCNA of pancreatic sections within the islet (magnification 40x). NDM + Ld: control rats treated daily with L. deterrimus extract for four weeks; DM: STZ-induced diabetic rats; DM + Ld: STZ-induced diabetic rats treated with L. deterrimus extract for four weeks.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4499631&req=5

fig5: The effect of L. deterrimus extract administration on the prosurvival pathway and β-cell proliferation in pancreatic islets. Light photomicrographs of immunohistochemical staining for pAkt and PCNA of pancreatic sections within the islet (magnification 40x). NDM + Ld: control rats treated daily with L. deterrimus extract for four weeks; DM: STZ-induced diabetic rats; DM + Ld: STZ-induced diabetic rats treated with L. deterrimus extract for four weeks.

Mentions: Phosphoinositide 3-kinases (PI3Ks) and its downstream effector protein kinase Akt mediate cellular survival signals that have an essential function in pancreatic β-cell survival [31]. Immunohistochemical staining with activated pAkt shown on Figure 5 revealed its presence in the islets of both control groups, whereas in diabetic islets pAkt-positive staining was weaker. Treatment of diabetic animals with Ld caused a considerable increase in pAkt staining that points to the stimulation of the prosurvival pathway in islets.


Protective Effects of the Mushroom Lactarius deterrimus Extract on Systemic Oxidative Stress and Pancreatic Islets in Streptozotocin-Induced Diabetic Rats.

Mihailović M, Arambašić Јovanović J, Uskoković A, Grdović N, Dinić S, Vidović S, Poznanović G, Mujić I, Vidaković M - J Diabetes Res (2015)

The effect of L. deterrimus extract administration on the prosurvival pathway and β-cell proliferation in pancreatic islets. Light photomicrographs of immunohistochemical staining for pAkt and PCNA of pancreatic sections within the islet (magnification 40x). NDM + Ld: control rats treated daily with L. deterrimus extract for four weeks; DM: STZ-induced diabetic rats; DM + Ld: STZ-induced diabetic rats treated with L. deterrimus extract for four weeks.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499631&req=5

fig5: The effect of L. deterrimus extract administration on the prosurvival pathway and β-cell proliferation in pancreatic islets. Light photomicrographs of immunohistochemical staining for pAkt and PCNA of pancreatic sections within the islet (magnification 40x). NDM + Ld: control rats treated daily with L. deterrimus extract for four weeks; DM: STZ-induced diabetic rats; DM + Ld: STZ-induced diabetic rats treated with L. deterrimus extract for four weeks.
Mentions: Phosphoinositide 3-kinases (PI3Ks) and its downstream effector protein kinase Akt mediate cellular survival signals that have an essential function in pancreatic β-cell survival [31]. Immunohistochemical staining with activated pAkt shown on Figure 5 revealed its presence in the islets of both control groups, whereas in diabetic islets pAkt-positive staining was weaker. Treatment of diabetic animals with Ld caused a considerable increase in pAkt staining that points to the stimulation of the prosurvival pathway in islets.

Bottom Line: In addition to a systemic antioxidant effect, the administration of the extract to diabetic rats also had a positive localized effect on pancreatic islets where it decreased AGE formation, and increased the expression of chemokine CXCL12 protein that mediates the restoration of β-cell population through the activation of the serine/threonine-specific Akt protein kinase prosurvival pathway.As a result, the numbers of proliferating cell nuclear antigen- (PCNA-) and insulin-positive β-cells were increased.These results show that the ability of the L. deterrimus extract to alleviate oxidative stress and increase β-cell mass represents a therapeutic potential for diabetes management.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.

ABSTRACT
The aim of this study was to assess the in vivo effects of the extract of the medicinal mushroom, Lactarius deterrimus, when administered (60 mg/kg, i.p.) daily for four weeks to streptozotocin- (STZ-) induced diabetic rats. Diabetic rats treated with the L. deterrimus extract displayed several improved biochemical parameters in the circulation: reduced hyperglycemia, lower triglyceride concentration and reduced glycated hemoglobin, glycated serum protein, and advanced glycation end product (AGE) levels. This treatment also adjusted the diabetes-induced redox imbalance. Thus, higher activities of the antioxidative enzymes, superoxide dismutase, and catalase in the circulation were accompanied by increased levels of free intracellular thiols and glutathionylated proteins after treatment with the L. deterrimus extract. In addition to a systemic antioxidant effect, the administration of the extract to diabetic rats also had a positive localized effect on pancreatic islets where it decreased AGE formation, and increased the expression of chemokine CXCL12 protein that mediates the restoration of β-cell population through the activation of the serine/threonine-specific Akt protein kinase prosurvival pathway. As a result, the numbers of proliferating cell nuclear antigen- (PCNA-) and insulin-positive β-cells were increased. These results show that the ability of the L. deterrimus extract to alleviate oxidative stress and increase β-cell mass represents a therapeutic potential for diabetes management.

No MeSH data available.


Related in: MedlinePlus