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Protective Effects of the Mushroom Lactarius deterrimus Extract on Systemic Oxidative Stress and Pancreatic Islets in Streptozotocin-Induced Diabetic Rats.

Mihailović M, Arambašić Јovanović J, Uskoković A, Grdović N, Dinić S, Vidović S, Poznanović G, Mujić I, Vidaković M - J Diabetes Res (2015)

Bottom Line: In addition to a systemic antioxidant effect, the administration of the extract to diabetic rats also had a positive localized effect on pancreatic islets where it decreased AGE formation, and increased the expression of chemokine CXCL12 protein that mediates the restoration of β-cell population through the activation of the serine/threonine-specific Akt protein kinase prosurvival pathway.As a result, the numbers of proliferating cell nuclear antigen- (PCNA-) and insulin-positive β-cells were increased.These results show that the ability of the L. deterrimus extract to alleviate oxidative stress and increase β-cell mass represents a therapeutic potential for diabetes management.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.

ABSTRACT
The aim of this study was to assess the in vivo effects of the extract of the medicinal mushroom, Lactarius deterrimus, when administered (60 mg/kg, i.p.) daily for four weeks to streptozotocin- (STZ-) induced diabetic rats. Diabetic rats treated with the L. deterrimus extract displayed several improved biochemical parameters in the circulation: reduced hyperglycemia, lower triglyceride concentration and reduced glycated hemoglobin, glycated serum protein, and advanced glycation end product (AGE) levels. This treatment also adjusted the diabetes-induced redox imbalance. Thus, higher activities of the antioxidative enzymes, superoxide dismutase, and catalase in the circulation were accompanied by increased levels of free intracellular thiols and glutathionylated proteins after treatment with the L. deterrimus extract. In addition to a systemic antioxidant effect, the administration of the extract to diabetic rats also had a positive localized effect on pancreatic islets where it decreased AGE formation, and increased the expression of chemokine CXCL12 protein that mediates the restoration of β-cell population through the activation of the serine/threonine-specific Akt protein kinase prosurvival pathway. As a result, the numbers of proliferating cell nuclear antigen- (PCNA-) and insulin-positive β-cells were increased. These results show that the ability of the L. deterrimus extract to alleviate oxidative stress and increase β-cell mass represents a therapeutic potential for diabetes management.

No MeSH data available.


Related in: MedlinePlus

The effect of administration of the L. deterrimus extract on histological changes and immunohistochemical localization of insulin and CXCL12 in pancreatic islets. HE: hematoxylin and eosin staining of pancreatic sections; light photomicrographs of insulin, CXCL12, and CXCR4 immunohistochemical staining of pancreatic sections within islets (magnification 40x). NDM: control rats; NDM + Ld: control rats treated daily with L. deterrimus extract for four weeks; DM: STZ-induced diabetic rats; DM + Ld: STZ-induced diabetic rats treated with L. deterrimus extract for four weeks.
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fig3: The effect of administration of the L. deterrimus extract on histological changes and immunohistochemical localization of insulin and CXCL12 in pancreatic islets. HE: hematoxylin and eosin staining of pancreatic sections; light photomicrographs of insulin, CXCL12, and CXCR4 immunohistochemical staining of pancreatic sections within islets (magnification 40x). NDM: control rats; NDM + Ld: control rats treated daily with L. deterrimus extract for four weeks; DM: STZ-induced diabetic rats; DM + Ld: STZ-induced diabetic rats treated with L. deterrimus extract for four weeks.

Mentions: Hematoxylin and eosin sections (Figure 3) show that the pancreas of control rats is comprised of numerous, compactly arranged cells in the islets of Langerhans that appear as dense cords. The pancreas of diabetic rats had smaller pancreatic islets with lower numbers of β-cells, displaying increased vacuolation and clumped cells. Ld-treated diabetic rats resembled more closely normal islet cell architecture, which is suggestive of a protective role of Ld on the pancreas of diabetic rats (Figure 3, HE). Immunohistochemical staining with insulin revealed disorganized islets in diabetic rats, with unevenly distributed insulin-positive cells in comparison to control islets that displayed strong insulin immunostaining (Figure 3, insulin). The Ld treatment increased the number of insulin-positive cells in the islets of diabetic rats (Figure 3, insulin).


Protective Effects of the Mushroom Lactarius deterrimus Extract on Systemic Oxidative Stress and Pancreatic Islets in Streptozotocin-Induced Diabetic Rats.

Mihailović M, Arambašić Јovanović J, Uskoković A, Grdović N, Dinić S, Vidović S, Poznanović G, Mujić I, Vidaković M - J Diabetes Res (2015)

The effect of administration of the L. deterrimus extract on histological changes and immunohistochemical localization of insulin and CXCL12 in pancreatic islets. HE: hematoxylin and eosin staining of pancreatic sections; light photomicrographs of insulin, CXCL12, and CXCR4 immunohistochemical staining of pancreatic sections within islets (magnification 40x). NDM: control rats; NDM + Ld: control rats treated daily with L. deterrimus extract for four weeks; DM: STZ-induced diabetic rats; DM + Ld: STZ-induced diabetic rats treated with L. deterrimus extract for four weeks.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499631&req=5

fig3: The effect of administration of the L. deterrimus extract on histological changes and immunohistochemical localization of insulin and CXCL12 in pancreatic islets. HE: hematoxylin and eosin staining of pancreatic sections; light photomicrographs of insulin, CXCL12, and CXCR4 immunohistochemical staining of pancreatic sections within islets (magnification 40x). NDM: control rats; NDM + Ld: control rats treated daily with L. deterrimus extract for four weeks; DM: STZ-induced diabetic rats; DM + Ld: STZ-induced diabetic rats treated with L. deterrimus extract for four weeks.
Mentions: Hematoxylin and eosin sections (Figure 3) show that the pancreas of control rats is comprised of numerous, compactly arranged cells in the islets of Langerhans that appear as dense cords. The pancreas of diabetic rats had smaller pancreatic islets with lower numbers of β-cells, displaying increased vacuolation and clumped cells. Ld-treated diabetic rats resembled more closely normal islet cell architecture, which is suggestive of a protective role of Ld on the pancreas of diabetic rats (Figure 3, HE). Immunohistochemical staining with insulin revealed disorganized islets in diabetic rats, with unevenly distributed insulin-positive cells in comparison to control islets that displayed strong insulin immunostaining (Figure 3, insulin). The Ld treatment increased the number of insulin-positive cells in the islets of diabetic rats (Figure 3, insulin).

Bottom Line: In addition to a systemic antioxidant effect, the administration of the extract to diabetic rats also had a positive localized effect on pancreatic islets where it decreased AGE formation, and increased the expression of chemokine CXCL12 protein that mediates the restoration of β-cell population through the activation of the serine/threonine-specific Akt protein kinase prosurvival pathway.As a result, the numbers of proliferating cell nuclear antigen- (PCNA-) and insulin-positive β-cells were increased.These results show that the ability of the L. deterrimus extract to alleviate oxidative stress and increase β-cell mass represents a therapeutic potential for diabetes management.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.

ABSTRACT
The aim of this study was to assess the in vivo effects of the extract of the medicinal mushroom, Lactarius deterrimus, when administered (60 mg/kg, i.p.) daily for four weeks to streptozotocin- (STZ-) induced diabetic rats. Diabetic rats treated with the L. deterrimus extract displayed several improved biochemical parameters in the circulation: reduced hyperglycemia, lower triglyceride concentration and reduced glycated hemoglobin, glycated serum protein, and advanced glycation end product (AGE) levels. This treatment also adjusted the diabetes-induced redox imbalance. Thus, higher activities of the antioxidative enzymes, superoxide dismutase, and catalase in the circulation were accompanied by increased levels of free intracellular thiols and glutathionylated proteins after treatment with the L. deterrimus extract. In addition to a systemic antioxidant effect, the administration of the extract to diabetic rats also had a positive localized effect on pancreatic islets where it decreased AGE formation, and increased the expression of chemokine CXCL12 protein that mediates the restoration of β-cell population through the activation of the serine/threonine-specific Akt protein kinase prosurvival pathway. As a result, the numbers of proliferating cell nuclear antigen- (PCNA-) and insulin-positive β-cells were increased. These results show that the ability of the L. deterrimus extract to alleviate oxidative stress and increase β-cell mass represents a therapeutic potential for diabetes management.

No MeSH data available.


Related in: MedlinePlus