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Protective Effects of the Mushroom Lactarius deterrimus Extract on Systemic Oxidative Stress and Pancreatic Islets in Streptozotocin-Induced Diabetic Rats.

Mihailović M, Arambašić Јovanović J, Uskoković A, Grdović N, Dinić S, Vidović S, Poznanović G, Mujić I, Vidaković M - J Diabetes Res (2015)

Bottom Line: In addition to a systemic antioxidant effect, the administration of the extract to diabetic rats also had a positive localized effect on pancreatic islets where it decreased AGE formation, and increased the expression of chemokine CXCL12 protein that mediates the restoration of β-cell population through the activation of the serine/threonine-specific Akt protein kinase prosurvival pathway.As a result, the numbers of proliferating cell nuclear antigen- (PCNA-) and insulin-positive β-cells were increased.These results show that the ability of the L. deterrimus extract to alleviate oxidative stress and increase β-cell mass represents a therapeutic potential for diabetes management.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.

ABSTRACT
The aim of this study was to assess the in vivo effects of the extract of the medicinal mushroom, Lactarius deterrimus, when administered (60 mg/kg, i.p.) daily for four weeks to streptozotocin- (STZ-) induced diabetic rats. Diabetic rats treated with the L. deterrimus extract displayed several improved biochemical parameters in the circulation: reduced hyperglycemia, lower triglyceride concentration and reduced glycated hemoglobin, glycated serum protein, and advanced glycation end product (AGE) levels. This treatment also adjusted the diabetes-induced redox imbalance. Thus, higher activities of the antioxidative enzymes, superoxide dismutase, and catalase in the circulation were accompanied by increased levels of free intracellular thiols and glutathionylated proteins after treatment with the L. deterrimus extract. In addition to a systemic antioxidant effect, the administration of the extract to diabetic rats also had a positive localized effect on pancreatic islets where it decreased AGE formation, and increased the expression of chemokine CXCL12 protein that mediates the restoration of β-cell population through the activation of the serine/threonine-specific Akt protein kinase prosurvival pathway. As a result, the numbers of proliferating cell nuclear antigen- (PCNA-) and insulin-positive β-cells were increased. These results show that the ability of the L. deterrimus extract to alleviate oxidative stress and increase β-cell mass represents a therapeutic potential for diabetes management.

No MeSH data available.


Related in: MedlinePlus

The effect of L. deterrimus extract administration on the content of free –SH (a), level of protein bound glutathione (GSSP) (b), and activities of antioxidative enzymes (c) in the circulation. NDM: control rats; NDM + Ld: control rats treated daily with L. deterrimus extract for four weeks; DM: STZ-induced diabetic rats; DM + Ld: STZ-induced diabetic rats treated with L. deterrimus extract for four weeks. The values are presented as the mean ± S.E.M.; values not sharing a common superscript letter differ significantly at P < 0.05.
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fig2: The effect of L. deterrimus extract administration on the content of free –SH (a), level of protein bound glutathione (GSSP) (b), and activities of antioxidative enzymes (c) in the circulation. NDM: control rats; NDM + Ld: control rats treated daily with L. deterrimus extract for four weeks; DM: STZ-induced diabetic rats; DM + Ld: STZ-induced diabetic rats treated with L. deterrimus extract for four weeks. The values are presented as the mean ± S.E.M.; values not sharing a common superscript letter differ significantly at P < 0.05.

Mentions: The redox status in experimental rats was estimated by measuring the levels of free intracellular thiols (Figure 2(a)) and GSSP (Figure 2(b)) that are directly linked to the redox state of the cell [25, 26]. These experiments revealed that the treatment with Ld significantly improved the redox parameters in the circulation. While the level of serum protein –SH groups was significantly decreased in diabetic rats, the treatment with Ld restored the –SH content almost to the control level. The –SH content was not significantly changed when control rats were administered the extract. As can be observed in Figure 2(b), the 1.3-fold increase in the level of GSSP in diabetic rats as compared to the control level was brought to the control level by the administration of Ld. The GSSP level was not significantly changed when healthy rats were administered Ld.


Protective Effects of the Mushroom Lactarius deterrimus Extract on Systemic Oxidative Stress and Pancreatic Islets in Streptozotocin-Induced Diabetic Rats.

Mihailović M, Arambašić Јovanović J, Uskoković A, Grdović N, Dinić S, Vidović S, Poznanović G, Mujić I, Vidaković M - J Diabetes Res (2015)

The effect of L. deterrimus extract administration on the content of free –SH (a), level of protein bound glutathione (GSSP) (b), and activities of antioxidative enzymes (c) in the circulation. NDM: control rats; NDM + Ld: control rats treated daily with L. deterrimus extract for four weeks; DM: STZ-induced diabetic rats; DM + Ld: STZ-induced diabetic rats treated with L. deterrimus extract for four weeks. The values are presented as the mean ± S.E.M.; values not sharing a common superscript letter differ significantly at P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499631&req=5

fig2: The effect of L. deterrimus extract administration on the content of free –SH (a), level of protein bound glutathione (GSSP) (b), and activities of antioxidative enzymes (c) in the circulation. NDM: control rats; NDM + Ld: control rats treated daily with L. deterrimus extract for four weeks; DM: STZ-induced diabetic rats; DM + Ld: STZ-induced diabetic rats treated with L. deterrimus extract for four weeks. The values are presented as the mean ± S.E.M.; values not sharing a common superscript letter differ significantly at P < 0.05.
Mentions: The redox status in experimental rats was estimated by measuring the levels of free intracellular thiols (Figure 2(a)) and GSSP (Figure 2(b)) that are directly linked to the redox state of the cell [25, 26]. These experiments revealed that the treatment with Ld significantly improved the redox parameters in the circulation. While the level of serum protein –SH groups was significantly decreased in diabetic rats, the treatment with Ld restored the –SH content almost to the control level. The –SH content was not significantly changed when control rats were administered the extract. As can be observed in Figure 2(b), the 1.3-fold increase in the level of GSSP in diabetic rats as compared to the control level was brought to the control level by the administration of Ld. The GSSP level was not significantly changed when healthy rats were administered Ld.

Bottom Line: In addition to a systemic antioxidant effect, the administration of the extract to diabetic rats also had a positive localized effect on pancreatic islets where it decreased AGE formation, and increased the expression of chemokine CXCL12 protein that mediates the restoration of β-cell population through the activation of the serine/threonine-specific Akt protein kinase prosurvival pathway.As a result, the numbers of proliferating cell nuclear antigen- (PCNA-) and insulin-positive β-cells were increased.These results show that the ability of the L. deterrimus extract to alleviate oxidative stress and increase β-cell mass represents a therapeutic potential for diabetes management.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.

ABSTRACT
The aim of this study was to assess the in vivo effects of the extract of the medicinal mushroom, Lactarius deterrimus, when administered (60 mg/kg, i.p.) daily for four weeks to streptozotocin- (STZ-) induced diabetic rats. Diabetic rats treated with the L. deterrimus extract displayed several improved biochemical parameters in the circulation: reduced hyperglycemia, lower triglyceride concentration and reduced glycated hemoglobin, glycated serum protein, and advanced glycation end product (AGE) levels. This treatment also adjusted the diabetes-induced redox imbalance. Thus, higher activities of the antioxidative enzymes, superoxide dismutase, and catalase in the circulation were accompanied by increased levels of free intracellular thiols and glutathionylated proteins after treatment with the L. deterrimus extract. In addition to a systemic antioxidant effect, the administration of the extract to diabetic rats also had a positive localized effect on pancreatic islets where it decreased AGE formation, and increased the expression of chemokine CXCL12 protein that mediates the restoration of β-cell population through the activation of the serine/threonine-specific Akt protein kinase prosurvival pathway. As a result, the numbers of proliferating cell nuclear antigen- (PCNA-) and insulin-positive β-cells were increased. These results show that the ability of the L. deterrimus extract to alleviate oxidative stress and increase β-cell mass represents a therapeutic potential for diabetes management.

No MeSH data available.


Related in: MedlinePlus