Limits...
β-Elemene Inhibits Cell Proliferation by Regulating the Expression and Activity of Topoisomerases I and IIα in Human Hepatocarcinoma HepG-2 Cells.

Gong M, Liu Y, Zhang J, Gao YJ, Zhai PP, Su X, Li X, Li Y, Hou L, Cui XN - Biomed Res Int (2015)

Bottom Line: To investigate the effects of β-Elemene (β-ELE) on the proliferation, apoptosis, and topoisomerase I (TOPO I) and topoisomerase IIα (TOPO IIα) expression and activity of human hepatocarcinoma HepG-2 cells.After treatment with β-ELE, morphological alterations of HepG-2 cells were observed under an inverted microscope.Supercoiled pBR322 and kDNA were also used to determine the direct effect of β-ELE on DNA breaks. β-ELE significantly inhibited HepG-2 cell proliferation in a dose- and time-dependent manner. β-ELE also induced tumor cell arrest at S phase, induced cell apoptosis, and downregulated the protein expression of TOPO I and TOPO IIα in a dose-dependent manner. β-ELE also inhibited TOPO I- and TOPO IIα-mediated DNA relaxation but did not directly induce DNA breakage at any concentration. β-ELE could inhibit the proliferation of HepG-2 cells and interfere with the expression and activity of TOPO I and TOPO IIα.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, China.

ABSTRACT

Objective: To investigate the effects of β-Elemene (β-ELE) on the proliferation, apoptosis, and topoisomerase I (TOPO I) and topoisomerase IIα (TOPO IIα) expression and activity of human hepatocarcinoma HepG-2 cells.

Methods: After treatment with β-ELE, morphological alterations of HepG-2 cells were observed under an inverted microscope. Cell proliferation was assessed using an MTT assay, cell cycles were analyzed using flow cytometry, and apoptosis was detected by Annexin V/PI staining. The expression of TOPO I and TOPO IIα was analyzed by Western blot techniques, and their activity was measured using the TOPO I-mediated, supercoiled pBR322 DNA relaxation and TOPO IIα-mediated Kinetoplast DNA (kDNA) decatenation assays, respectively. Supercoiled pBR322 and kDNA were also used to determine the direct effect of β-ELE on DNA breaks.

Results: β-ELE significantly inhibited HepG-2 cell proliferation in a dose- and time-dependent manner. β-ELE also induced tumor cell arrest at S phase, induced cell apoptosis, and downregulated the protein expression of TOPO I and TOPO IIα in a dose-dependent manner. β-ELE also inhibited TOPO I- and TOPO IIα-mediated DNA relaxation but did not directly induce DNA breakage at any concentration.

Conclusion: β-ELE could inhibit the proliferation of HepG-2 cells and interfere with the expression and activity of TOPO I and TOPO IIα.

No MeSH data available.


Related in: MedlinePlus

The role of β-ELE (β-Elemene) in cell morphological changes (×40). Figure 2 shows that β-ELE plays a role in the morphological changes of HepG-2 cells, as observed under an optical inverted microscope. (a) shows the control group, which is without β-ELE. (b)–(f) show cells exposed to different concentrations of β-ELE (20, 40, 60, 80, and 100 μg/mL, resp.). Compared with the normal cells, the treated cells were markedly shrunken, the cell membrane was partially broken, and some cells showed necrolysis and partial karyopyknosis. These changes were more severe and more evident with increasing concentrations of β-ELE. The culture medium showed a large amount of cell debris and few intact cells when cells were treated with 100 μg/mL.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4499621&req=5

fig2: The role of β-ELE (β-Elemene) in cell morphological changes (×40). Figure 2 shows that β-ELE plays a role in the morphological changes of HepG-2 cells, as observed under an optical inverted microscope. (a) shows the control group, which is without β-ELE. (b)–(f) show cells exposed to different concentrations of β-ELE (20, 40, 60, 80, and 100 μg/mL, resp.). Compared with the normal cells, the treated cells were markedly shrunken, the cell membrane was partially broken, and some cells showed necrolysis and partial karyopyknosis. These changes were more severe and more evident with increasing concentrations of β-ELE. The culture medium showed a large amount of cell debris and few intact cells when cells were treated with 100 μg/mL.

Mentions: Observation under an optical inverted microscope revealed that β-ELE induced morphological changes in HepG-2 cells, as shown in Figure 2. After 72 h of culture, cells of the control group were adherent, spindle-shaped, and tightly packed (Figure 2(a)). Compared with the control group, cells treated with different concentrations of β-ELE (20, 40, 60, 80, and 100 μg/mL) were markedly shrunken, the cell membrane was partially broken, and some cells showed necrolysis and partial karyopyknosis. These changes were more severe or more evident with increasing concentrations of β-ELE (Figures 2(b)–2(f)). The culture medium showed a large amount of cell debris, and few cells were intact when treated with 100 μg/mL.


β-Elemene Inhibits Cell Proliferation by Regulating the Expression and Activity of Topoisomerases I and IIα in Human Hepatocarcinoma HepG-2 Cells.

Gong M, Liu Y, Zhang J, Gao YJ, Zhai PP, Su X, Li X, Li Y, Hou L, Cui XN - Biomed Res Int (2015)

The role of β-ELE (β-Elemene) in cell morphological changes (×40). Figure 2 shows that β-ELE plays a role in the morphological changes of HepG-2 cells, as observed under an optical inverted microscope. (a) shows the control group, which is without β-ELE. (b)–(f) show cells exposed to different concentrations of β-ELE (20, 40, 60, 80, and 100 μg/mL, resp.). Compared with the normal cells, the treated cells were markedly shrunken, the cell membrane was partially broken, and some cells showed necrolysis and partial karyopyknosis. These changes were more severe and more evident with increasing concentrations of β-ELE. The culture medium showed a large amount of cell debris and few intact cells when cells were treated with 100 μg/mL.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499621&req=5

fig2: The role of β-ELE (β-Elemene) in cell morphological changes (×40). Figure 2 shows that β-ELE plays a role in the morphological changes of HepG-2 cells, as observed under an optical inverted microscope. (a) shows the control group, which is without β-ELE. (b)–(f) show cells exposed to different concentrations of β-ELE (20, 40, 60, 80, and 100 μg/mL, resp.). Compared with the normal cells, the treated cells were markedly shrunken, the cell membrane was partially broken, and some cells showed necrolysis and partial karyopyknosis. These changes were more severe and more evident with increasing concentrations of β-ELE. The culture medium showed a large amount of cell debris and few intact cells when cells were treated with 100 μg/mL.
Mentions: Observation under an optical inverted microscope revealed that β-ELE induced morphological changes in HepG-2 cells, as shown in Figure 2. After 72 h of culture, cells of the control group were adherent, spindle-shaped, and tightly packed (Figure 2(a)). Compared with the control group, cells treated with different concentrations of β-ELE (20, 40, 60, 80, and 100 μg/mL) were markedly shrunken, the cell membrane was partially broken, and some cells showed necrolysis and partial karyopyknosis. These changes were more severe or more evident with increasing concentrations of β-ELE (Figures 2(b)–2(f)). The culture medium showed a large amount of cell debris, and few cells were intact when treated with 100 μg/mL.

Bottom Line: To investigate the effects of β-Elemene (β-ELE) on the proliferation, apoptosis, and topoisomerase I (TOPO I) and topoisomerase IIα (TOPO IIα) expression and activity of human hepatocarcinoma HepG-2 cells.After treatment with β-ELE, morphological alterations of HepG-2 cells were observed under an inverted microscope.Supercoiled pBR322 and kDNA were also used to determine the direct effect of β-ELE on DNA breaks. β-ELE significantly inhibited HepG-2 cell proliferation in a dose- and time-dependent manner. β-ELE also induced tumor cell arrest at S phase, induced cell apoptosis, and downregulated the protein expression of TOPO I and TOPO IIα in a dose-dependent manner. β-ELE also inhibited TOPO I- and TOPO IIα-mediated DNA relaxation but did not directly induce DNA breakage at any concentration. β-ELE could inhibit the proliferation of HepG-2 cells and interfere with the expression and activity of TOPO I and TOPO IIα.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, China.

ABSTRACT

Objective: To investigate the effects of β-Elemene (β-ELE) on the proliferation, apoptosis, and topoisomerase I (TOPO I) and topoisomerase IIα (TOPO IIα) expression and activity of human hepatocarcinoma HepG-2 cells.

Methods: After treatment with β-ELE, morphological alterations of HepG-2 cells were observed under an inverted microscope. Cell proliferation was assessed using an MTT assay, cell cycles were analyzed using flow cytometry, and apoptosis was detected by Annexin V/PI staining. The expression of TOPO I and TOPO IIα was analyzed by Western blot techniques, and their activity was measured using the TOPO I-mediated, supercoiled pBR322 DNA relaxation and TOPO IIα-mediated Kinetoplast DNA (kDNA) decatenation assays, respectively. Supercoiled pBR322 and kDNA were also used to determine the direct effect of β-ELE on DNA breaks.

Results: β-ELE significantly inhibited HepG-2 cell proliferation in a dose- and time-dependent manner. β-ELE also induced tumor cell arrest at S phase, induced cell apoptosis, and downregulated the protein expression of TOPO I and TOPO IIα in a dose-dependent manner. β-ELE also inhibited TOPO I- and TOPO IIα-mediated DNA relaxation but did not directly induce DNA breakage at any concentration.

Conclusion: β-ELE could inhibit the proliferation of HepG-2 cells and interfere with the expression and activity of TOPO I and TOPO IIα.

No MeSH data available.


Related in: MedlinePlus