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Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress.

Ferlazzo N, Visalli G, Smeriglio A, Cirmi S, Lombardo GE, Campiglia P, Di Pietro A, Navarra M - Evid Based Complement Alternat Med (2015)

Bottom Line: Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites.The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells.Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Sciences and Products for Health, University of Messina, Viale Annunziata, 98168 Messina, Italy.

ABSTRACT
It has been reported that oxidant/antioxidant imbalance triggers cell damage that in turn causes a number of lung diseases. Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites. The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells. First we tested the antioxidant properties of both extracts in cell-free experimental models and then we assayed their capability to prevent the cytotoxic effects induced by H2O2. Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2. Our data indicate that the mix of flavonoids present in both bergamot and orange juices may be of use in preventing oxidative cell injury and pave the way for further research into a novel healthy approach to avoid lung disorders.

No MeSH data available.


Related in: MedlinePlus

Protective effects of FFBJ and FFOJ on DNA damage induced by H2O2. (a) Levels of 8-oxo-dG are measured as emission signals of fluorochrome FITC-labeled avidin collected in the FL-1 channel. The graph reports the mean of fluorescence expressed in arbitrary fluorescence units (AFU) of three independent experiments. ∗∗∗P < 0.001 versus control cultures and ##P < 0.01 versus H2O2-treated cells. (b) Images of comet assay captured by fluorescence microscopy at a magnification of 400x. A representative experiment that was replicated three times with similar results is shown. Percentages of tail DNA (TDNA) are indicated.
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fig9: Protective effects of FFBJ and FFOJ on DNA damage induced by H2O2. (a) Levels of 8-oxo-dG are measured as emission signals of fluorochrome FITC-labeled avidin collected in the FL-1 channel. The graph reports the mean of fluorescence expressed in arbitrary fluorescence units (AFU) of three independent experiments. ∗∗∗P < 0.001 versus control cultures and ##P < 0.01 versus H2O2-treated cells. (b) Images of comet assay captured by fluorescence microscopy at a magnification of 400x. A representative experiment that was replicated three times with similar results is shown. Percentages of tail DNA (TDNA) are indicated.

Mentions: Levels of 8-oxo-dG and DNA strand breaks were measured to study the effectiveness of the Citrus extracts to restrain DNA-oxidative damage. The results of cytofluorimetric analysis using a FITC-labelled avidin probe are reported in Figure 9. This shows that 18 h of treatment with FFBJ or FFOJ did not induce DNA oxidation since the emission values roughly overlapped those recorded in control cells. Despite the massive DNA damage induced by 2 h of H2O2 200 μM incubation, the pretreatment with FFBJ or FFOJ significantly decreased oxidative DNA damage by about 1.5 fold (P < 0.01; Figure 9(a)). Similar results were obtained using the comet assay (Figure 9(b)).


Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress.

Ferlazzo N, Visalli G, Smeriglio A, Cirmi S, Lombardo GE, Campiglia P, Di Pietro A, Navarra M - Evid Based Complement Alternat Med (2015)

Protective effects of FFBJ and FFOJ on DNA damage induced by H2O2. (a) Levels of 8-oxo-dG are measured as emission signals of fluorochrome FITC-labeled avidin collected in the FL-1 channel. The graph reports the mean of fluorescence expressed in arbitrary fluorescence units (AFU) of three independent experiments. ∗∗∗P < 0.001 versus control cultures and ##P < 0.01 versus H2O2-treated cells. (b) Images of comet assay captured by fluorescence microscopy at a magnification of 400x. A representative experiment that was replicated three times with similar results is shown. Percentages of tail DNA (TDNA) are indicated.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499611&req=5

fig9: Protective effects of FFBJ and FFOJ on DNA damage induced by H2O2. (a) Levels of 8-oxo-dG are measured as emission signals of fluorochrome FITC-labeled avidin collected in the FL-1 channel. The graph reports the mean of fluorescence expressed in arbitrary fluorescence units (AFU) of three independent experiments. ∗∗∗P < 0.001 versus control cultures and ##P < 0.01 versus H2O2-treated cells. (b) Images of comet assay captured by fluorescence microscopy at a magnification of 400x. A representative experiment that was replicated three times with similar results is shown. Percentages of tail DNA (TDNA) are indicated.
Mentions: Levels of 8-oxo-dG and DNA strand breaks were measured to study the effectiveness of the Citrus extracts to restrain DNA-oxidative damage. The results of cytofluorimetric analysis using a FITC-labelled avidin probe are reported in Figure 9. This shows that 18 h of treatment with FFBJ or FFOJ did not induce DNA oxidation since the emission values roughly overlapped those recorded in control cells. Despite the massive DNA damage induced by 2 h of H2O2 200 μM incubation, the pretreatment with FFBJ or FFOJ significantly decreased oxidative DNA damage by about 1.5 fold (P < 0.01; Figure 9(a)). Similar results were obtained using the comet assay (Figure 9(b)).

Bottom Line: Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites.The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells.Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Sciences and Products for Health, University of Messina, Viale Annunziata, 98168 Messina, Italy.

ABSTRACT
It has been reported that oxidant/antioxidant imbalance triggers cell damage that in turn causes a number of lung diseases. Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites. The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells. First we tested the antioxidant properties of both extracts in cell-free experimental models and then we assayed their capability to prevent the cytotoxic effects induced by H2O2. Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2. Our data indicate that the mix of flavonoids present in both bergamot and orange juices may be of use in preventing oxidative cell injury and pave the way for further research into a novel healthy approach to avoid lung disorders.

No MeSH data available.


Related in: MedlinePlus