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Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress.

Ferlazzo N, Visalli G, Smeriglio A, Cirmi S, Lombardo GE, Campiglia P, Di Pietro A, Navarra M - Evid Based Complement Alternat Med (2015)

Bottom Line: Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites.The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells.Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Sciences and Products for Health, University of Messina, Viale Annunziata, 98168 Messina, Italy.

ABSTRACT
It has been reported that oxidant/antioxidant imbalance triggers cell damage that in turn causes a number of lung diseases. Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites. The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells. First we tested the antioxidant properties of both extracts in cell-free experimental models and then we assayed their capability to prevent the cytotoxic effects induced by H2O2. Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2. Our data indicate that the mix of flavonoids present in both bergamot and orange juices may be of use in preventing oxidative cell injury and pave the way for further research into a novel healthy approach to avoid lung disorders.

No MeSH data available.


Related in: MedlinePlus

CLSM analysis of mitochondrial membrane potential. A549 cells were grown on cell slides and after treatment with FFBJ or FFOJ were incubated with H2O2. Mitochondrial membrane potential was detected by R123 staining. Red fluorescence indicates functional mitochondria. Images captured at 400x magnification are shown as representative from three independent experiments.
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fig8: CLSM analysis of mitochondrial membrane potential. A549 cells were grown on cell slides and after treatment with FFBJ or FFOJ were incubated with H2O2. Mitochondrial membrane potential was detected by R123 staining. Red fluorescence indicates functional mitochondria. Images captured at 400x magnification are shown as representative from three independent experiments.

Mentions: The CLSM observations confirmed the results from FACS analyses (Figure 8), showing that the presence of FFBJ or FFOJ reduced the fall in Δψm, as revealed by the increased emitted fluorescence.


Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress.

Ferlazzo N, Visalli G, Smeriglio A, Cirmi S, Lombardo GE, Campiglia P, Di Pietro A, Navarra M - Evid Based Complement Alternat Med (2015)

CLSM analysis of mitochondrial membrane potential. A549 cells were grown on cell slides and after treatment with FFBJ or FFOJ were incubated with H2O2. Mitochondrial membrane potential was detected by R123 staining. Red fluorescence indicates functional mitochondria. Images captured at 400x magnification are shown as representative from three independent experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499611&req=5

fig8: CLSM analysis of mitochondrial membrane potential. A549 cells were grown on cell slides and after treatment with FFBJ or FFOJ were incubated with H2O2. Mitochondrial membrane potential was detected by R123 staining. Red fluorescence indicates functional mitochondria. Images captured at 400x magnification are shown as representative from three independent experiments.
Mentions: The CLSM observations confirmed the results from FACS analyses (Figure 8), showing that the presence of FFBJ or FFOJ reduced the fall in Δψm, as revealed by the increased emitted fluorescence.

Bottom Line: Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites.The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells.Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Sciences and Products for Health, University of Messina, Viale Annunziata, 98168 Messina, Italy.

ABSTRACT
It has been reported that oxidant/antioxidant imbalance triggers cell damage that in turn causes a number of lung diseases. Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites. The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells. First we tested the antioxidant properties of both extracts in cell-free experimental models and then we assayed their capability to prevent the cytotoxic effects induced by H2O2. Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2. Our data indicate that the mix of flavonoids present in both bergamot and orange juices may be of use in preventing oxidative cell injury and pave the way for further research into a novel healthy approach to avoid lung disorders.

No MeSH data available.


Related in: MedlinePlus