Limits...
Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress.

Ferlazzo N, Visalli G, Smeriglio A, Cirmi S, Lombardo GE, Campiglia P, Di Pietro A, Navarra M - Evid Based Complement Alternat Med (2015)

Bottom Line: Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites.The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells.Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Sciences and Products for Health, University of Messina, Viale Annunziata, 98168 Messina, Italy.

ABSTRACT
It has been reported that oxidant/antioxidant imbalance triggers cell damage that in turn causes a number of lung diseases. Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites. The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells. First we tested the antioxidant properties of both extracts in cell-free experimental models and then we assayed their capability to prevent the cytotoxic effects induced by H2O2. Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2. Our data indicate that the mix of flavonoids present in both bergamot and orange juices may be of use in preventing oxidative cell injury and pave the way for further research into a novel healthy approach to avoid lung disorders.

No MeSH data available.


Related in: MedlinePlus

Effect of FFBJ or FFOJ on mitochondrial membrane potential in H2O2-treated cells. The cells treated with FFBJ or FFOJ and then exposed to 200 μM H2O2 for 2 h were loaded with R123 probe. Fluorescence was followed by flow cytometry. The shift of the curves to the left of the graph indicates the reduction of Δψm. Results from FFBJ (a) or FFOJ (b) exposure. In (c) are reported data from (a) and (b) expressed as percentage of increase (%Δ) of R123 emission values in FFBJ- or FFOJ-pretreated cells subsequently exposed to H2O2 compared to H2O2-stressed cells. ∗P < 0.05 and ∗∗P < 0.01 versus H2O2-treated cells.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4499611&req=5

fig7: Effect of FFBJ or FFOJ on mitochondrial membrane potential in H2O2-treated cells. The cells treated with FFBJ or FFOJ and then exposed to 200 μM H2O2 for 2 h were loaded with R123 probe. Fluorescence was followed by flow cytometry. The shift of the curves to the left of the graph indicates the reduction of Δψm. Results from FFBJ (a) or FFOJ (b) exposure. In (c) are reported data from (a) and (b) expressed as percentage of increase (%Δ) of R123 emission values in FFBJ- or FFOJ-pretreated cells subsequently exposed to H2O2 compared to H2O2-stressed cells. ∗P < 0.05 and ∗∗P < 0.01 versus H2O2-treated cells.

Mentions: Since mitochondrial membrane phospholipids are key targets for lipid peroxidation involving highly polyunsaturated side chains, we further evaluated the ability of both extracts to restrain mitochondrial impairment. Figure 7 shows that incubation of A549 cells with H2O2 caused a drop in Δψm, as indicated by the peak of the R123 probe fluorescence emission on the left of the graph (Figures 7(a) and 7(b)). The presence of FFBJ or FFOJ lessened the ROS-induced mitochondrial impairment observed in stressed cells, reducing the number of cells with lower R123 fluorescence emission values. This is clearly shown in Figure 7(c) which presents the increases in weighted-averages fluorescence found in cultures preincubated with the Citrus extracts prior to H2O2 incubation, in comparison to the non-pretreated oxidatively stressed cells, set at 0 (P < 0.05 and P < 0.01 for 25 and 50 μg/mL of both extracts, resp.).


Flavonoid Fraction of Orange and Bergamot Juices Protect Human Lung Epithelial Cells from Hydrogen Peroxide-Induced Oxidative Stress.

Ferlazzo N, Visalli G, Smeriglio A, Cirmi S, Lombardo GE, Campiglia P, Di Pietro A, Navarra M - Evid Based Complement Alternat Med (2015)

Effect of FFBJ or FFOJ on mitochondrial membrane potential in H2O2-treated cells. The cells treated with FFBJ or FFOJ and then exposed to 200 μM H2O2 for 2 h were loaded with R123 probe. Fluorescence was followed by flow cytometry. The shift of the curves to the left of the graph indicates the reduction of Δψm. Results from FFBJ (a) or FFOJ (b) exposure. In (c) are reported data from (a) and (b) expressed as percentage of increase (%Δ) of R123 emission values in FFBJ- or FFOJ-pretreated cells subsequently exposed to H2O2 compared to H2O2-stressed cells. ∗P < 0.05 and ∗∗P < 0.01 versus H2O2-treated cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4499611&req=5

fig7: Effect of FFBJ or FFOJ on mitochondrial membrane potential in H2O2-treated cells. The cells treated with FFBJ or FFOJ and then exposed to 200 μM H2O2 for 2 h were loaded with R123 probe. Fluorescence was followed by flow cytometry. The shift of the curves to the left of the graph indicates the reduction of Δψm. Results from FFBJ (a) or FFOJ (b) exposure. In (c) are reported data from (a) and (b) expressed as percentage of increase (%Δ) of R123 emission values in FFBJ- or FFOJ-pretreated cells subsequently exposed to H2O2 compared to H2O2-stressed cells. ∗P < 0.05 and ∗∗P < 0.01 versus H2O2-treated cells.
Mentions: Since mitochondrial membrane phospholipids are key targets for lipid peroxidation involving highly polyunsaturated side chains, we further evaluated the ability of both extracts to restrain mitochondrial impairment. Figure 7 shows that incubation of A549 cells with H2O2 caused a drop in Δψm, as indicated by the peak of the R123 probe fluorescence emission on the left of the graph (Figures 7(a) and 7(b)). The presence of FFBJ or FFOJ lessened the ROS-induced mitochondrial impairment observed in stressed cells, reducing the number of cells with lower R123 fluorescence emission values. This is clearly shown in Figure 7(c) which presents the increases in weighted-averages fluorescence found in cultures preincubated with the Citrus extracts prior to H2O2 incubation, in comparison to the non-pretreated oxidatively stressed cells, set at 0 (P < 0.05 and P < 0.01 for 25 and 50 μg/mL of both extracts, resp.).

Bottom Line: Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites.The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells.Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2.

View Article: PubMed Central - PubMed

Affiliation: Department of Drug Sciences and Products for Health, University of Messina, Viale Annunziata, 98168 Messina, Italy.

ABSTRACT
It has been reported that oxidant/antioxidant imbalance triggers cell damage that in turn causes a number of lung diseases. Flavonoids are known for their health benefits, and Citrus fruits juices are one of the main food sources of these secondary plant metabolites. The present study was designed to evaluate the effect of the flavonoid fraction of bergamot and orange juices, on H2O2-induced oxidative stress in human lung epithelial A549 cells. First we tested the antioxidant properties of both extracts in cell-free experimental models and then we assayed their capability to prevent the cytotoxic effects induced by H2O2. Our results demonstrated that both Citrus juice extracts reduce the generation of reactive oxygen species and membrane lipid peroxidation, improve mitochondrial functionality, and prevent DNA-oxidative damage in A549 cells incubated with H2O2. Our data indicate that the mix of flavonoids present in both bergamot and orange juices may be of use in preventing oxidative cell injury and pave the way for further research into a novel healthy approach to avoid lung disorders.

No MeSH data available.


Related in: MedlinePlus